Magnesium in PDB 4e7z: Myosin VI (Md) Pre-Powerstroke State, P21 Crystal Form

The structure of Myosin VI (Md) Pre-Powerstroke State, P21 Crystal Form, PDB code: 4e7z was solved by T.Isabet, H.L.Sweeney, A.Houdusse, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.65 / 2.30
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	98.148, 93.319, 101.875, 90.00, 90.60, 90.00
R / Rfree (%)	17.6 / 23.1

The structure of Myosin VI (Md) Pre-Powerstroke State, P21 Crystal Form also contains other interesting chemical elements:

Vanadium

(V)

2 atoms

The binding sites of Magnesium atom in the Myosin VI (Md) Pre-Powerstroke State, P21 Crystal Form (pdb code 4e7z). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Myosin VI (Md) Pre-Powerstroke State, P21 Crystal Form, PDB code: 4e7z:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Myosin VI (Md) Pre-Powerstroke State, P21 Crystal Form


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Myosin VI (Md) Pre-Powerstroke State, P21 Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg803 b:32.4 occ:1.00 O2 A:VO4802 2.2 26.5 1.0 O1B A:ADP801 2.4 20.7 1.0 OG1 A:THR158 2.4 30.7 1.0 O A:HOH933 2.5 28.4 1.0 OG A:SER204 2.5 28.5 1.0 O A:HOH1035 2.5 22.5 1.0 CB A:THR158 2.9 26.1 1.0 CB A:SER204 3.2 27.9 1.0 PB A:ADP801 3.7 24.5 1.0 N A:THR158 3.9 26.6 1.0 OD1 A:ASP456 3.9 29.2 1.0 V A:VO4802 3.9 22.7 1.0 CA A:THR158 3.9 29.5 1.0 N A:SER204 3.9 26.1 1.0 OD2 A:ASP456 4.0 29.3 1.0 CG2 A:THR158 4.0 26.1 1.0 O2B A:ADP801 4.1 26.1 1.0 O2A A:ADP801 4.2 26.1 1.0 CA A:SER204 4.2 23.9 1.0 CG A:ASP456 4.3 31.4 1.0 O A:HOH1136 4.4 44.5 1.0 O3A A:ADP801 4.6 24.1 1.0 PA A:ADP801 4.7 25.7 1.0 O1A A:ADP801 4.7 25.0 1.0 O3B A:ADP801 4.7 23.8 1.0 O A:HOH1069 4.7 35.6 1.0 O A:ASN202 4.7 26.8 1.0 ND2 A:ASN194 4.7 25.6 1.0 O1 A:VO4802 4.7 27.5 1.0 O4 A:VO4802 4.8 25.5 1.0 O A:ILE457 4.8 25.1 1.0 C A:LYS157 4.9 26.2 1.0 O A:HOH932 5.0 33.1 1.0

Magnesium binding site 2 out of 2 in the Myosin VI (Md) Pre-Powerstroke State, P21 Crystal Form


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Myosin VI (Md) Pre-Powerstroke State, P21 Crystal Form within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg803 b:32.1 occ:1.00 O2 B:VO4802 2.3 30.1 1.0 O1B B:ADP801 2.4 23.8 1.0 O B:HOH983 2.4 28.3 1.0 OG1 B:THR158 2.4 28.8 1.0 O B:HOH971 2.5 24.9 1.0 OG B:SER204 2.5 24.3 1.0 CB B:SER204 3.2 25.4 1.0 CB B:THR158 3.3 27.5 1.0 PB B:ADP801 3.4 26.0 1.0 V B:VO4802 3.6 23.6 1.0 O2B B:ADP801 3.7 24.2 1.0 OD2 B:ASP456 3.8 34.1 1.0 N B:SER204 3.9 26.9 1.0 N B:THR158 3.9 26.3 1.0 OD1 B:ASP456 4.0 33.6 1.0 CA B:THR158 4.1 24.1 1.0 CA B:SER204 4.1 25.7 1.0 O4 B:VO4802 4.2 24.4 1.0 CG B:ASP456 4.3 35.0 1.0 O2A B:ADP801 4.4 25.9 1.0 O3B B:ADP801 4.4 28.2 1.0 CG2 B:THR158 4.4 26.0 1.0 O3A B:ADP801 4.5 24.1 1.0 O B:ILE457 4.5 27.3 1.0 O1 B:VO4802 4.6 22.0 1.0 PA B:ADP801 4.7 25.2 1.0 O1A B:ADP801 4.8 23.9 1.0 O B:HOH926 4.8 34.8 1.0 CB B:LYS157 4.8 25.7 1.0 C B:LYS157 4.9 26.9 1.0 O B:ASN202 4.9 24.7 1.0 ND2 B:ASN194 4.9 27.5 1.0 CE B:LYS157 5.0 23.1 1.0

J.Menetrey, T.Isabet, V.Ropars, M.Mukherjea, O.Pylypenko, X.Liu, J.Perez, P.Vachette, H.L.Sweeney, A.M.Houdusse. Processive Steps in the Reverse Direction Require Uncoupling of the Lead Head Lever Arm of Myosin VI. Mol.Cell V. 48 75 2012.
ISSN: ISSN 1097-2765
PubMed: 22940248
DOI: 10.1016/J.MOLCEL.2012.07.034