Magnesium in PDB 4efn: Crystal Structure of H-Ras Q61L in Complex with Gppnhp (State 1)

The structure of Crystal Structure of H-Ras Q61L in Complex with Gppnhp (State 1), PDB code: 4efn was solved by S.Muraoka, F.Shima, M.Araki, T.Inoue, A.Yoshimoto, Y.Ijiri, N.Seki, A.Tamura, T.Kumasaka, M.Yamamoto, T.Kataoka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.96 / 2.30
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	34.782, 82.006, 121.251, 90.00, 90.00, 90.00
R / Rfree (%)	18.3 / 23.7

The binding sites of Magnesium atom in the Crystal Structure of H-Ras Q61L in Complex with Gppnhp (State 1) (pdb code 4efn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of H-Ras Q61L in Complex with Gppnhp (State 1), PDB code: 4efn:

Magnesium binding site 1 out of 1 in the Crystal Structure of H-Ras Q61L in Complex with Gppnhp (State 1)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of H-Ras Q61L in Complex with Gppnhp (State 1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:13.3 occ:1.00 O A:HOH1001 1.9 12.0 1.0 O1B A:GNP201 2.0 12.2 1.0 O1G A:GNP201 2.0 14.5 1.0 O A:HOH1003 2.1 16.6 1.0 OG A:SER17 2.1 7.0 1.0 O A:HOH1002 2.2 12.6 1.0 PB A:GNP201 3.2 12.2 1.0 PG A:GNP201 3.3 14.5 1.0 CB A:SER17 3.3 8.6 1.0 O A:HOH1010 3.4 23.6 1.0 N3B A:GNP201 3.4 12.9 1.0 OD2 A:ASP57 3.8 16.4 1.0 N A:SER17 3.9 9.5 1.0 OD1 A:ASP57 4.0 16.4 1.0 CA A:SER17 4.1 8.7 1.0 O2B A:GNP201 4.1 11.5 1.0 O3G A:GNP201 4.2 14.5 1.0 CG A:ASP57 4.3 15.6 1.0 O3A A:GNP201 4.3 12.5 1.0 O A:HOH1047 4.4 28.4 1.0 O1A A:GNP201 4.4 13.0 1.0 O2G A:GNP201 4.4 15.7 1.0 O A:PRO34 4.5 31.3 1.0 CE A:LYS16 4.6 9.1 1.0 PA A:GNP201 4.7 13.2 1.0 CB A:LYS16 4.8 9.0 1.0 C A:LYS16 4.9 9.5 1.0 O2A A:GNP201 4.9 13.2 1.0 O A:THR58 4.9 14.5 1.0

S.Muraoka, F.Shima, M.Araki, T.Inoue, A.Yoshimoto, Y.Ijiri, N.Seki, A.Tamura, T.Kumasaka, M.Yamamoto, T.Kataoka. Crystal Structures of the State 1 Conformations of the Gtp-Bound H-Ras Protein and Its Oncogenic G12V and Q61L Mutants Febs Lett. V. 586 1715 2012.
ISSN: ISSN 0014-5793
PubMed: 22584058
DOI: 10.1016/J.FEBSLET.2012.04.058