Magnesium in PDB 4ekc: Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C)

All present enzymatic activity of Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C):
3.6.5.1;

The structure of Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C), PDB code: 4ekc was solved by J.J.G.Tesmer, M.R.Nance, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.98 / 7.40
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	139.603, 124.983, 97.196, 90.00, 124.13, 90.00
R / Rfree (%)	15.8 / 22

The structure of Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C) also contains other interesting chemical elements:

Fluorine	(F)	8 atoms
Aluminium	(Al)	2 atoms

The binding sites of Magnesium atom in the Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C) (pdb code 4ekc). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C), PDB code: 4ekc:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:69.1 occ:1.00 O A:HOH502 1.7 75.2 1.0 F3 A:ALF401 1.8 45.4 1.0 O A:HOH503 1.9 0.5 1.0 O2B A:GDP400 1.9 63.4 1.0 OG1 A:THR186 2.4 0.8 1.0 OG A:SER53 2.5 76.1 1.0 PB A:GDP400 3.2 62.7 1.0 CB A:THR186 3.3 0.6 1.0 AL A:ALF401 3.3 43.5 1.0 CB A:SER53 3.3 76.4 1.0 O2A A:GDP400 3.5 74.6 1.0 F1 A:ALF401 3.5 44.6 1.0 O3B A:GDP400 3.6 61.4 1.0 N A:SER53 3.6 76.5 1.0 N A:THR186 3.7 0.3 1.0 F2 A:ALF401 4.0 46.6 1.0 CA A:SER53 4.0 77.0 1.0 O A:HOH501 4.1 43.7 1.0 PA A:GDP400 4.1 74.8 1.0 CA A:THR186 4.1 0.8 1.0 O3A A:GDP400 4.2 68.5 1.0 O1B A:GDP400 4.3 62.3 1.0 O1A A:GDP400 4.3 74.7 1.0 OD1 A:ASP205 4.3 0.3 1.0 O A:VAL206 4.4 0.8 1.0 CG2 A:THR186 4.4 0.8 1.0 CB A:LYS52 4.5 74.8 1.0 O A:VAL184 4.5 0.8 1.0 C A:PRO185 4.6 0.3 1.0 C A:LYS52 4.7 75.5 1.0 OD2 A:ASP205 4.7 0.1 1.0 CE A:LYS52 4.9 75.3 1.0 CA A:PRO185 4.9 0.7 1.0 NZ A:LYS52 4.9 74.2 1.0 F4 A:ALF401 5.0 44.3 1.0 CG A:ASP205 5.0 0.7 1.0 CA A:LYS52 5.0 74.3 1.0

Magnesium binding site 2 out of 2 in the Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Human Regulator of G Protein Signaling 2 (RGS2) in Complex with Murine Galpha-Q(R183C) within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg402 b:64.5 occ:1.00 O C:HOH502 1.8 80.2 1.0 F3 C:ALF401 1.8 60.5 1.0 O C:HOH503 1.9 75.5 1.0 O2B C:GDP400 1.9 59.7 1.0 OG1 C:THR186 2.4 0.4 1.0 OG C:SER53 2.4 77.8 1.0 PB C:GDP400 3.2 59.4 1.0 CB C:THR186 3.3 0.4 1.0 AL C:ALF401 3.3 60.2 1.0 CB C:SER53 3.3 78.0 1.0 O2A C:GDP400 3.5 63.0 1.0 F1 C:ALF401 3.5 61.5 1.0 N C:SER53 3.6 79.1 1.0 O3B C:GDP400 3.6 60.4 1.0 N C:THR186 3.8 0.6 1.0 F2 C:ALF401 4.0 61.9 1.0 CA C:SER53 4.0 78.8 1.0 PA C:GDP400 4.1 62.6 1.0 CA C:THR186 4.2 0.7 1.0 O C:HOH501 4.2 65.9 1.0 O3A C:GDP400 4.2 61.1 1.0 O1B C:GDP400 4.3 59.8 1.0 OD1 C:ASP205 4.3 95.0 1.0 O1A C:GDP400 4.3 63.7 1.0 CB C:LYS52 4.4 78.7 1.0 O C:VAL206 4.4 96.8 1.0 CG2 C:THR186 4.4 0.4 1.0 O C:VAL184 4.6 0.8 1.0 C C:LYS52 4.6 79.6 1.0 C C:PRO185 4.6 0.3 1.0 OD2 C:ASP205 4.7 95.8 1.0 CE C:LYS52 4.8 77.9 1.0 NZ C:LYS52 4.9 77.5 1.0 CA C:LYS52 4.9 79.0 1.0 CG C:ASP205 4.9 95.5 1.0 CA C:PRO185 5.0 0.0 1.0 F4 C:ALF401 5.0 61.0 1.0

M.R.Nance, B.Kreutz, V.M.Tesmer, R.Sterne-Marr, T.Kozasa, J.J.Tesmer. Structural and Functional Analysis of the Regulator of G Protein Signaling 2-G Alpha Q Complex. Structure V. 21 438 2013.
ISSN: ISSN 0969-2126
PubMed: 23434405
DOI: 10.1016/J.STR.2012.12.016