Magnesium in PDB 4ffb: A Tog:Alpha/Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase

All present enzymatic activity of A Tog:Alpha/Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase:
3.6.5.6;

The structure of A Tog:Alpha/Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase, PDB code: 4ffb was solved by P.Ayaz, X.Ye, P.Huddleston, C.A.Brautigam, L.M.Rice, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.95 / 2.88
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	89.120, 98.040, 91.366, 90.00, 100.31, 90.00
R / Rfree (%)	20.7 / 26.7

The binding sites of Magnesium atom in the A Tog:Alpha/Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase (pdb code 4ffb). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the A Tog:Alpha/Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase, PDB code: 4ffb:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the A Tog:Alpha/Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of A Tog:Alpha/Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:37.8 occ:1.00 O2B A:GTP502 2.1 35.8 1.0 O2G A:GTP502 2.1 35.8 1.0 OE2 A:GLU72 2.6 44.6 1.0 CD A:GLU72 3.4 45.5 1.0 PB A:GTP502 3.4 35.8 1.0 PG A:GTP502 3.4 35.8 1.0 OE1 A:GLN11 3.6 36.3 1.0 CB A:GLN11 3.7 36.0 1.0 OE1 A:GLU72 3.8 46.1 1.0 O2A A:GTP502 3.8 35.8 1.0 O3B A:GTP502 3.8 35.8 1.0 NZ B:LYS252 4.0 41.4 1.0 OD2 A:ASP70 4.2 36.2 1.0 O1B A:GTP502 4.3 35.8 1.0 O3G A:GTP502 4.3 35.8 1.0 O1G A:GTP502 4.4 35.8 1.0 N A:GLN11 4.5 36.2 1.0 CG A:GLU72 4.5 46.2 1.0 O3A A:GTP502 4.5 35.8 1.0 OD2 A:ASP99 4.5 41.9 1.0 CD A:GLN11 4.6 35.9 1.0 OD1 A:ASP70 4.6 37.1 1.0 CA A:GLN11 4.6 36.1 1.0 CG A:GLN11 4.7 35.9 1.0 PA A:GTP502 4.7 35.8 1.0 CG A:ASP70 4.9 36.7 1.0 CB A:ASP99 4.9 41.8 1.0

Magnesium binding site 2 out of 2 in the A Tog:Alpha/Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of A Tog:Alpha/Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg501 b:33.3 occ:1.00 O2B B:GTP502 2.6 57.5 1.0 O2G B:GTP502 2.8 57.5 1.0 O3G B:GTP502 2.9 57.5 1.0 O3A B:GTP502 3.2 57.5 1.0 PG B:GTP502 3.3 57.5 1.0 PB B:GTP502 3.4 57.5 1.0 OE1 B:GLU69 3.4 90.0 1.0 OD1 B:ASP67 3.8 61.5 1.0 CB B:GLN11 3.8 87.0 1.0 O3B B:GTP502 3.8 57.5 1.0 OD2 B:ASP67 3.9 63.7 1.0 N B:GLN11 3.9 85.4 1.0 CG B:ASP67 4.2 62.1 1.0 CA B:GLN11 4.4 86.3 1.0 CD B:GLU69 4.6 92.1 1.0 PA B:GTP502 4.6 57.5 1.0 O1G B:GTP502 4.7 57.5 1.0 O1B B:GTP502 4.8 57.5 1.0 C B:GLY10 4.8 87.0 1.0 O2A B:GTP502 4.9 57.5 1.0 CA B:GLY10 5.0 86.2 1.0

P.Ayaz, X.Ye, P.Huddleston, C.A.Brautigam, L.M.Rice. A Tog: Alpha Beta-Tubulin Complex Structure Reveals Conformation-Based Mechanisms For A Microtubule Polymerase. Science V. 337 857 2012.
ISSN: ISSN 0036-8075
PubMed: 22904013
DOI: 10.1126/SCIENCE.1221698