Atomistry » Magnesium » PDB 4gnk-4gyp » 4gwa
Atomistry »
  Magnesium »
    PDB 4gnk-4gyp »
      4gwa »

Magnesium in PDB 4gwa: Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata

Enzymatic activity of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata

All present enzymatic activity of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata:
3.2.1.91;

Protein crystallography data

The structure of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata, PDB code: 4gwa was solved by J.E.Mcgeehan, R.N.A.Martin, S.D.Streeter, S.M.Cragg, M.J.Guille, K.M.Schnorr, M.Kern, N.C.Bruce, S.J.Mcqueen-Mason, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 1.60
Space group P 1
Cell size a, b, c (Å), α, β, γ (°) 51.920, 54.050, 71.970, 94.13, 95.70, 94.64
R / Rfree (%) 14.8 / 18.4

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata (pdb code 4gwa). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 8 binding sites of Magnesium where determined in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata, PDB code: 4gwa:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8;

Magnesium binding site 1 out of 8 in 4gwa

Go back to Magnesium Binding Sites List in 4gwa
Magnesium binding site 1 out of 8 in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg501

b:28.2
occ:1.00
 O A:HOH881 2.0 31.5 1.0
O A:HOH890 2.1 21.5 1.0
O A:HOH994 2.1 29.2 1.0
O A:HOH813 2.1 24.2 1.0
O A:HOH995 2.1 32.8 1.0
OD2 A:ASP80 2.2 23.7 1.0
CG A:ASP80 3.1 18.2 1.0
OD1 A:ASP80 3.5 16.8 1.0
O A:HOH863 4.0 30.3 1.0
OG1 A:THR83 4.1 15.2 1.0
CB A:ASP80 4.4 14.8 1.0

Magnesium binding site 2 out of 8 in 4gwa

Go back to Magnesium Binding Sites List in 4gwa
Magnesium binding site 2 out of 8 in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg502

b:24.9
occ:1.00
 O A:HOH733 2.0 23.0 1.0
O A:HOH752 2.1 26.7 1.0
O A:HOH832 2.1 23.1 1.0
O A:HOH697 2.1 20.8 1.0
O A:HOH983 2.1 26.9 1.0
OE2 A:GLU139 2.1 17.1 1.0
CD A:GLU139 3.2 14.5 1.0
OE1 A:GLU139 3.5 12.6 1.0
O A:HOH973 4.1 17.7 1.0
NH1 A:ARG40 4.1 23.5 1.0
O A:ILE140 4.2 10.6 1.0
O A:HOH1069 4.3 34.9 1.0
O A:HOH613 4.4 17.4 1.0
N A:ILE140 4.4 10.2 1.0
CG A:GLU139 4.5 11.7 1.0
CB A:ALA133 4.9 11.7 1.0

Magnesium binding site 3 out of 8 in 4gwa

Go back to Magnesium Binding Sites List in 4gwa
Magnesium binding site 3 out of 8 in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg503

b:29.1
occ:1.00
 O A:HOH992 1.9 30.1 1.0
O A:HOH989 2.1 30.8 1.0
O A:HOH993 2.1 30.7 1.0
O A:HOH991 2.2 29.5 1.0
O A:HOH990 2.2 29.4 1.0
OE1 A:GLU226 4.2 27.4 1.0
OD2 A:ASP211 4.3 20.0 1.0
OE2 A:GLU226 4.3 31.0 1.0
CD A:GLU226 4.7 25.3 1.0
CG A:ASP211 4.8 19.0 1.0

Magnesium binding site 4 out of 8 in 4gwa

Go back to Magnesium Binding Sites List in 4gwa
Magnesium binding site 4 out of 8 in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg501

b:19.5
occ:1.00
 O B:HOH947 2.0 22.3 1.0
OE2 B:GLU139 2.0 14.6 1.0
O B:HOH945 2.1 22.2 1.0
O B:HOH944 2.1 20.2 1.0
O B:HOH744 2.2 25.2 1.0
O B:HOH946 2.2 21.8 1.0
CD B:GLU139 3.0 15.1 1.0
OE1 B:GLU139 3.4 15.7 1.0
NH1 B:ARG40 4.0 26.6 1.0
O B:HOH711 4.0 21.7 1.0
O B:ILE140 4.2 12.8 1.0
O B:HOH827 4.2 40.4 1.0
O B:HOH915 4.3 39.2 1.0
O B:HOH1070 4.3 36.9 1.0
CG B:GLU139 4.3 12.7 1.0
O B:HOH623 4.4 23.2 1.0
N B:ILE140 4.5 11.0 1.0
CB B:ALA133 4.8 11.4 1.0

Magnesium binding site 5 out of 8 in 4gwa

Go back to Magnesium Binding Sites List in 4gwa
Magnesium binding site 5 out of 8 in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg502

b:24.1
occ:1.00
 O B:HOH959 2.0 26.8 1.0
O B:HOH1060 2.1 32.7 1.0
O B:HOH961 2.1 28.8 1.0
O B:HOH960 2.2 34.0 1.0
OE2 B:GLU62 4.1 36.6 1.0
OE1 B:GLU62 4.9 38.5 1.0
CD B:GLU62 4.9 37.8 1.0

Magnesium binding site 6 out of 8 in 4gwa

Go back to Magnesium Binding Sites List in 4gwa
Magnesium binding site 6 out of 8 in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg503

b:27.2
occ:1.00
 O B:HOH957 1.9 30.1 1.0
O B:HOH954 2.0 24.5 1.0
O B:HOH955 2.1 29.9 1.0
O B:HOH956 2.1 27.6 1.0
O A:HOH986 2.3 36.5 1.0
OG B:SER114 4.1 13.7 1.0
O B:HOH654 4.3 17.4 1.0
OG1 B:THR117 4.3 15.9 1.0
O A:HOH908 4.4 31.9 1.0
O B:HOH825 4.4 30.0 1.0
O B:HOH1012 4.4 30.1 1.0
O A:HOH945 4.5 40.6 1.0
O B:HOH784 4.5 25.3 1.0
O B:VAL116 4.6 15.1 1.0
O A:HOH849 4.6 46.1 1.0
CB B:SER114 4.6 13.3 1.0
O A:HOH696 4.7 15.9 1.0
O B:HOH935 4.9 38.0 1.0

Magnesium binding site 7 out of 8 in 4gwa

Go back to Magnesium Binding Sites List in 4gwa
Magnesium binding site 7 out of 8 in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg504

b:28.0
occ:1.00
 O B:HOH1092 1.9 32.3 1.0
O B:HOH953 2.0 32.2 1.0
O B:HOH857 2.1 35.8 1.0
O B:HOH952 2.1 22.2 1.0
O B:HOH634 2.2 25.4 1.0
O B:HOH951 2.4 30.5 1.0
O B:HOH938 4.1 31.3 1.0
O B:HOH992 4.3 27.6 1.0
O B:TYR256 4.3 11.5 1.0
OG1 B:THR257 4.4 12.1 1.0
O B:ASP254 4.4 15.8 1.0
O B:HOH633 4.5 20.0 1.0
CG2 B:THR257 4.9 11.8 1.0

Magnesium binding site 8 out of 8 in 4gwa

Go back to Magnesium Binding Sites List in 4gwa
Magnesium binding site 8 out of 8 in the Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of Crystal Structure of A GH7 Family Cellobiohydrolase From Limnoria Quadripunctata within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg505

b:27.3
occ:1.00
 O B:HOH1090 1.8 29.1 1.0
O B:HOH941 2.0 27.2 1.0
O B:HOH921 2.1 27.8 1.0
O B:HOH978 2.1 28.7 1.0
O B:HOH674 2.2 18.5 1.0
O B:HOH988 2.2 30.7 1.0
OD2 B:ASP151 3.9 17.7 1.0
O B:HOH763 4.1 25.8 1.0
OD1 B:ASP151 4.1 16.7 1.0
O B:HOH1074 4.3 46.5 1.0
OG1 B:THR307 4.3 14.7 1.0
CG B:ASP151 4.4 16.9 1.0
O B:HOH629 4.5 15.7 1.0
ND2 B:ASN443 4.5 21.5 1.0
CG2 B:THR307 4.9 13.5 1.0

Reference:

M.Kern, J.E.Mcgeehan, S.D.Streeter, R.N.Martin, K.Besser, L.Elias, W.Eborall, G.P.Malyon, C.M.Payne, M.E.Himmel, K.Schnorr, G.T.Beckham, S.M.Cragg, N.C.Bruce, S.J.Mcqueen-Mason. Structural Characterization of A Unique Marine Animal Family 7 Cellobiohydrolase Suggests A Mechanism of Cellulase Salt Tolerance Proc.Natl.Acad.Sci.Usa V. 110 10189 2013.
ISSN: ISSN 0027-8424
PubMed: 23733951
DOI: 10.1073/PNAS.1301502110
Page generated: Fri Aug 16 16:02:24 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy