Magnesium in PDB 4k9m: Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida

Enzymatic activity of Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida

All present enzymatic activity of Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida:
4.1.1.7;

Protein crystallography data

The structure of Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida, PDB code: 4k9m was solved by H.R.Brodkin, M.J.Mcleish, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	22.96 / 1.15
*Space group*	I 2 2 2
*Cell size a, b, c (Å), α, β, γ (°)*	81.192, 95.596, 137.037, 90.00, 90.00, 90.00
*R / R_free (%)*	14.1 / 15.1

Other elements in 4k9m:

The structure of Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida also contains other interesting chemical elements:

Calcium

(Ca)

1 atom

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida (pdb code 4k9m). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida, PDB code: 4k9m:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in 4k9m

Go back to

Magnesium Binding Sites List in 4k9m

Magnesium binding site 1 out of 3 in the Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida

Mono view

Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Mg603 b:21.5 occ:1.00	NE2	A:HIS144	2.7	9.9	1.0
	O	A:LEU174	2.7	8.5	1.0
	OG	A:SER140	2.7	8.0	1.0
	CD2	A:HIS144	3.5	9.5	1.0
	CB	A:ARG177	3.5	9.3	1.0
	NE2	A:GLN15	3.7	10.7	1.0
	CE1	A:HIS144	3.7	10.6	1.0
	C	A:LEU174	3.7	8.5	1.0
	CB	A:SER140	3.7	7.9	1.0
	CG	A:ARG177	3.8	9.3	1.0
	CD	A:ARG177	3.8	8.7	1.0
	CD1	A:LEU11	3.9	9.4	1.0
	CD	A:GLN15	4.0	10.3	1.0
	CA	A:LEU174	4.1	8.5	1.0
	CB	A:LEU174	4.2	8.6	1.0
	CA	A:SER140	4.4	7.2	1.0
	CG	A:GLN15	4.5	9.7	1.0
	N	A:ARG177	4.5	9.8	1.0
	CA	A:ARG177	4.5	9.7	1.0
	OE1	A:GLN15	4.6	11.5	1.0
	NH1	A:ARG177	4.6	7.2	0.8
	O	A:ARG177	4.6	10.5	1.0
	CG	A:HIS144	4.7	8.4	1.0
	O	A:SER140	4.8	7.0	1.0
	ND1	A:HIS144	4.8	9.3	0.9
	NE	A:ARG177	4.9	8.0	1.0
	CD2	A:LEU174	4.9	8.7	1.0
	N	A:PHE175	4.9	8.8	1.0
	C	A:SER140	4.9	7.0	1.0
	O	A:PHE175	5.0	10.1	1.0

Magnesium binding site 2 out of 3 in 4k9m

Go back to

Magnesium Binding Sites List in 4k9m

Magnesium binding site 2 out of 3 in the Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida

Mono view

Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Mg604 b:6.8 occ:0.50	O	A:LEU118	2.5	6.9	1.0
	O	A:ARG120	2.5	7.7	0.9
	O	A:ASN117	2.7	7.8	1.0
	O	A:HOH718	3.2	10.1	0.5
	C	A:LEU118	3.3	6.8	1.0
	C	A:ARG120	3.7	7.2	1.0
	C	A:ASN117	3.8	7.0	1.0
	CA	A:LEU118	3.8	6.6	1.0
	N	A:ARG120	3.9	7.1	1.0
	N	A:PRO119	4.3	7.1	1.0
	N	A:LEU118	4.3	6.4	1.0
	CA	A:ARG120	4.3	7.6	1.0
	C	A:PRO119	4.4	7.5	1.0
	CE	A:MET79	4.4	8.7	0.5
	CB	A:ARG120	4.6	8.4	1.0
	CA	A:PRO119	4.7	7.8	1.0
	N	A:PRO121	4.7	7.3	1.0
	CD	A:PRO121	4.9	8.4	1.0

Magnesium binding site 3 out of 3 in 4k9m

Go back to

Magnesium Binding Sites List in 4k9m

Magnesium binding site 3 out of 3 in the Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida

Mono view

Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Mg605 b:13.0 occ:1.00	N	A:ASP187	3.2	11.7	1.0
	O	A:HOH944	3.4	23.0	1.0
	CG	A:ASP187	3.7	15.4	0.9
	CA	A:ASN186	3.8	10.3	1.0
	CB	A:ASP187	3.8	13.6	1.0
	CB	A:ASN186	3.9	11.3	1.0
	OD2	A:ASP187	3.9	15.9	0.6
	C	A:ASN186	4.0	11.0	1.0
	OD1	A:ASP187	4.0	16.3	0.8
	CA	A:ASP187	4.1	12.1	1.0
	O	A:HOH1132	4.5	39.8	1.0
	O	A:HOH1071	4.6	36.1	1.0
	NH1	A:ARG184	4.9	15.2	1.0
	O	A:HOH1102	5.0	37.8	1.0

Reference:

H.R.Brodkin, F.H.Andrews, A.C.Milne, G.A.Petsko, D.Ringe, M.J.Mcleish. Crystal Structure of the HIS281ASN Mutant of Benzoylformate Decarboxylase From Pseudomonas Putida To Be Published.

Page generated: Sat Aug 17 03:30:11 2024

Last articles

Zn in 9J0N
Zn in 9J0O
Zn in 9J0P
Zn in 9FJX
Zn in 9EKB
Zn in 9C0F
Zn in 9CAH
Zn in 9CH0
Zn in 9CH3
Zn in 9CH1

	© Copyright 2008-2020 by atomistry.com
Home \| Site Map \| Copyright \| Contact us \| Privacy