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Magnesium in PDB 4kjg: Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell

Enzymatic activity of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell

All present enzymatic activity of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell:
3.1.3.1;

Protein crystallography data

The structure of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell, PDB code: 4kjg was solved by K.Ghosh, R.K.Anumula, B.K.Laksmaiah, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.42 / 2.38
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 90.306, 167.170, 71.843, 90.00, 90.00, 90.00
R / Rfree (%) 16.4 / 20.4

Other elements in 4kjg:

The structure of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell also contains other interesting chemical elements:

Zinc (Zn) 4 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell (pdb code 4kjg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell, PDB code: 4kjg:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6;

Magnesium binding site 1 out of 6 in 4kjg

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Magnesium binding site 1 out of 6 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1003

b:54.4
occ:1.00
 OD2 A:ASP42 1.9 38.3 1.0
O A:HOH1288 2.4 47.2 1.0
O A:HOH1289 2.5 44.7 1.0
O A:HOH1266 2.5 45.1 1.0
OE2 A:GLU311 2.7 30.9 1.0
OG A:SER155 2.7 30.1 1.0
CG A:ASP42 3.1 27.9 1.0
CB A:SER155 3.6 17.4 1.0
CD A:GLU311 3.7 37.4 1.0
ND1 A:HIS153 3.8 21.2 1.0
OD1 A:ASP42 3.8 27.0 1.0
O A:HOH1111 4.0 37.0 1.0
OG A:SER92 4.0 27.8 1.0
CB A:ASP42 4.0 20.7 1.0
OE1 A:GLU311 4.1 31.8 1.0
CB A:SER92 4.1 18.9 1.0
N A:SER155 4.1 14.0 1.0
ZN A:ZN1002 4.3 34.2 0.8
CA A:SER155 4.5 14.5 1.0
CE1 A:HIS153 4.6 20.3 1.0
OD2 A:ASP357 4.7 24.4 1.0
O A:HOH1109 4.7 15.4 1.0
O A:GLY313 4.7 19.2 1.0
CA A:GLY313 4.8 15.3 1.0
CG A:HIS153 4.9 19.1 1.0

Magnesium binding site 2 out of 6 in 4kjg

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Magnesium binding site 2 out of 6 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1004

b:24.0
occ:1.00
 OD1 A:ASP285 2.2 24.5 1.0
O A:PHE269 2.3 18.7 1.0
OE1 A:GLU216 2.3 32.8 1.0
OE2 A:GLU270 2.3 24.7 1.0
O A:HOH1110 2.4 21.1 1.0
OE2 A:GLU216 2.8 30.6 1.0
CD A:GLU216 2.9 39.8 1.0
CD A:GLU270 3.3 35.7 1.0
CG A:ASP285 3.4 25.4 1.0
C A:PHE269 3.5 18.9 1.0
NZ A:LYS275 3.9 16.6 1.0
CG A:GLU270 4.0 22.7 1.0
O A:HOH1112 4.0 28.1 1.0
OD2 A:ASP285 4.1 31.5 1.0
OE1 A:GLU270 4.2 26.8 1.0
O A:HOH1108 4.3 27.6 1.0
CA A:PHE269 4.3 14.1 1.0
CB A:PHE269 4.3 15.4 1.0
CB A:ASP285 4.3 19.5 1.0
CG A:GLU216 4.4 25.1 1.0
N A:GLU270 4.5 16.5 1.0
O A:ALA284 4.6 27.7 1.0
O A:TRP248 4.6 18.7 1.0
CA A:GLU270 4.6 16.2 1.0
NH1 A:ARG204 4.6 16.9 1.0
CA A:ASP285 4.6 18.2 1.0
OH A:TYR217 4.7 35.1 1.0
NH2 A:ARG204 4.7 13.5 1.0
C A:ALA284 4.8 26.5 1.0
N A:ASP285 4.8 19.9 1.0
CB A:GLU270 4.9 17.2 1.0
CB A:ALA284 4.9 23.8 1.0

Magnesium binding site 3 out of 6 in 4kjg

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Magnesium binding site 3 out of 6 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg1005

b:49.8
occ:1.00
 O A:THR345 2.4 20.0 1.0
O A:SER342 2.5 17.5 1.0
O A:HOH1104 2.7 28.7 1.0
O A:HOH1113 3.1 32.2 1.0
C A:THR345 3.6 20.3 1.0
C A:SER342 3.7 19.5 1.0
CA A:ASN346 4.1 15.7 1.0
N A:ASN346 4.3 16.3 1.0
O A:HOH1105 4.4 22.8 1.0
OG A:SER342 4.5 35.1 1.0
N A:THR345 4.5 17.4 1.0
O A:HOH1106 4.5 33.6 1.0
N A:GLN343 4.6 17.2 1.0
CA A:SER342 4.6 15.7 1.0
CA A:GLN343 4.7 18.1 1.0
C A:GLN343 4.7 22.4 1.0
CA A:THR345 4.7 16.9 1.0
O A:GLN343 4.7 22.5 1.0
N A:GLU347 4.9 15.8 1.0

Magnesium binding site 4 out of 6 in 4kjg

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Magnesium binding site 4 out of 6 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg1003

b:41.4
occ:1.00
 OD2 B:ASP42 2.3 40.1 1.0
O B:HOH1111 2.5 26.2 1.0
OG B:SER155 2.5 24.7 1.0
O B:HOH1112 2.7 43.7 1.0
O B:HOH1108 2.9 27.6 1.0
OE2 B:GLU311 3.0 35.3 1.0
CB B:SER155 3.4 17.3 1.0
CG B:ASP42 3.4 29.0 1.0
ND1 B:HIS153 3.6 26.8 1.0
N B:SER155 3.7 15.8 1.0
O B:HOH1113 3.8 31.1 1.0
CB B:SER92 3.9 19.7 1.0
OG B:SER92 3.9 26.9 1.0
CD B:GLU311 3.9 38.1 1.0
OD1 B:ASP42 4.2 26.7 1.0
CA B:SER155 4.2 16.1 1.0
OE1 B:GLU311 4.2 27.9 1.0
CB B:ASP42 4.4 18.3 1.0
ZN B:ZN1002 4.5 32.5 0.8
CE1 B:HIS153 4.5 27.1 1.0
O B:HOH1107 4.5 17.0 1.0
CG B:HIS153 4.7 24.1 1.0
OD2 B:ASP357 4.7 22.4 1.0
N B:ALA154 4.8 18.2 1.0
C B:ALA154 4.8 20.7 1.0
CB B:HIS153 4.8 19.8 1.0
CB B:ALA154 4.8 18.1 1.0
CD B:PRO156 4.9 17.9 1.0

Magnesium binding site 5 out of 6 in 4kjg

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Magnesium binding site 5 out of 6 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg1004

b:29.4
occ:1.00
 OD1 B:ASP285 2.0 29.9 1.0
OE2 B:GLU270 2.3 15.3 1.0
O B:HOH1189 2.4 21.6 1.0
O B:PHE269 2.4 16.7 1.0
OE1 B:GLU216 2.5 48.7 1.0
OE2 B:GLU216 2.8 40.3 1.0
CD B:GLU216 3.0 49.6 1.0
CG B:ASP285 3.2 30.3 1.0
CD B:GLU270 3.3 31.8 1.0
O B:HOH1290 3.5 37.5 1.0
C B:PHE269 3.7 18.0 1.0
NZ B:LYS275 3.7 44.2 1.0
OD2 B:ASP285 4.0 31.2 1.0
CG B:GLU270 4.0 23.5 1.0
OE1 B:GLU270 4.2 31.4 1.0
CB B:ASP285 4.2 22.1 1.0
CB B:PHE269 4.4 11.4 1.0
O B:ALA284 4.4 26.8 1.0
CA B:PHE269 4.4 10.6 1.0
CG B:GLU216 4.5 34.1 1.0
CA B:ASP285 4.5 20.9 1.0
OH B:TYR217 4.6 39.7 1.0
N B:GLU270 4.7 15.4 1.0
O B:TRP248 4.7 22.6 1.0
C B:ALA284 4.8 26.8 1.0
CA B:GLU270 4.8 14.7 1.0
NH1 B:ARG204 4.8 16.6 1.0
N B:ASP285 4.8 21.5 1.0
NH2 B:ARG204 4.9 13.7 1.0

Magnesium binding site 6 out of 6 in 4kjg

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Magnesium binding site 6 out of 6 in the Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Structure of Rat Intestinal Alkaline Phosphatase Expressed in Insect Cell within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg1005

b:31.9
occ:1.00
 O B:THR345 2.3 22.1 1.0
O B:HOH1101 2.3 15.5 1.0
O B:SER342 2.4 21.4 1.0
O B:HOH1104 2.5 43.3 1.0
O B:HOH1109 2.6 26.8 1.0
C B:THR345 3.5 21.4 1.0
C B:SER342 3.6 21.2 1.0
O B:HOH1115 4.0 22.4 1.0
O B:HOH1116 4.0 42.2 1.0
CA B:ASN346 4.1 16.4 1.0
OG B:SER342 4.2 35.8 1.0
N B:ASN346 4.2 16.2 1.0
CA B:SER342 4.4 18.4 1.0
N B:THR345 4.5 19.1 1.0
N B:GLU347 4.6 17.9 1.0
N B:GLN343 4.6 16.4 1.0
CA B:THR345 4.6 18.7 1.0
C B:ASN346 4.8 19.2 1.0
CA B:GLN343 4.8 16.7 1.0
C B:GLN343 4.9 21.9 1.0
OG1 B:THR345 5.0 23.0 1.0
CB B:SER342 5.0 21.8 1.0

Reference:

K.Ghosh, D.Mazumder Tagore, R.Anumula, B.Lakshmaiah, P.P.Kumar, S.Singaram, T.Matan, S.Kallipatti, S.Selvam, P.Krishnamurthy, M.Ramarao. Crystal Structure of Rat Intestinal Alkaline Phosphatase - Role of Crown Domain in Mammalian Alkaline Phosphatases. J.Struct.Biol. V. 184 182 2013.
ISSN: ISSN 1047-8477
PubMed: 24076154
DOI: 10.1016/J.JSB.2013.09.017
Page generated: Sat Aug 17 03:39:56 2024

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