Magnesium in PDB 4ko8: Structure of P97 N-D1 R155H Mutant in Complex with Atpgs

All present enzymatic activity of Structure of P97 N-D1 R155H Mutant in Complex with Atpgs:
3.6.4.6;

The structure of Structure of P97 N-D1 R155H Mutant in Complex with Atpgs, PDB code: 4ko8 was solved by D.Xia, W.K.Tang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	98.11 / 1.98
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	134.206, 134.206, 182.886, 90.00, 90.00, 120.00
R / Rfree (%)	19.4 / 20.5

The binding sites of Magnesium atom in the Structure of P97 N-D1 R155H Mutant in Complex with Atpgs (pdb code 4ko8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of P97 N-D1 R155H Mutant in Complex with Atpgs, PDB code: 4ko8:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of P97 N-D1 R155H Mutant in Complex with Atpgs


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of P97 N-D1 R155H Mutant in Complex with Atpgs within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg801 b:37.5 occ:1.00 O A:HOH902 2.0 28.2 1.0 O A:HOH903 2.0 33.2 1.0 O2G A:AGS800 2.0 33.7 1.0 OG1 A:THR252 2.2 31.0 1.0 O1B A:AGS800 2.2 31.3 1.0 O A:HOH901 2.2 28.8 1.0 CB A:THR252 3.2 30.4 1.0 PG A:AGS800 3.3 32.7 1.0 PB A:AGS800 3.4 32.9 1.0 O3B A:AGS800 3.5 32.2 1.0 O2A A:AGS800 3.9 36.9 1.0 N A:THR252 4.0 30.4 1.0 OD2 A:ASP304 4.0 31.4 1.0 OD1 A:ASP304 4.1 31.4 1.0 O3G A:AGS800 4.1 32.1 1.0 CA A:THR252 4.1 30.4 1.0 O3A A:AGS800 4.3 34.0 1.0 CG2 A:THR252 4.3 30.1 1.0 OE2 A:GLU305 4.3 37.7 1.0 CG A:ASP304 4.5 31.0 1.0 O A:HOH981 4.5 39.4 1.0 O A:HOH1029 4.5 41.9 1.0 PA A:AGS800 4.5 34.5 1.0 O2B A:AGS800 4.6 31.6 1.0 CG A:GLU305 4.7 35.5 1.0 S1G A:AGS800 4.8 34.4 1.0 O1A A:AGS800 4.8 32.8 1.0 CD A:GLU305 4.9 35.7 1.0

Magnesium binding site 2 out of 2 in the Structure of P97 N-D1 R155H Mutant in Complex with Atpgs


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of P97 N-D1 R155H Mutant in Complex with Atpgs within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg801 b:43.9 occ:1.00 O2G B:AGS800 2.0 33.5 1.0 O1B B:AGS800 2.1 33.1 1.0 O B:HOH902 2.1 37.6 1.0 O B:HOH903 2.1 30.6 1.0 OG1 B:THR252 2.1 30.8 1.0 O B:HOH901 2.3 33.0 1.0 PG B:AGS800 3.2 34.4 1.0 CB B:THR252 3.2 30.6 1.0 PB B:AGS800 3.3 33.6 1.0 O3B B:AGS800 3.5 34.0 1.0 O2A B:AGS800 3.9 37.8 1.0 N B:THR252 3.9 30.5 1.0 OD2 B:ASP304 4.0 31.3 1.0 O3G B:AGS800 4.1 34.7 1.0 CA B:THR252 4.1 30.4 1.0 OD1 B:ASP304 4.1 31.4 1.0 O3A B:AGS800 4.1 35.3 1.0 O B:HOH996 4.2 40.4 1.0 CG2 B:THR252 4.3 30.1 1.0 O B:HOH966 4.4 48.8 1.0 OE2 B:GLU305 4.4 37.9 1.0 PA B:AGS800 4.4 36.6 1.0 CG B:ASP304 4.5 31.1 1.0 O2B B:AGS800 4.5 32.4 1.0 CG B:GLU305 4.8 35.5 1.0 S1G B:AGS800 4.8 35.5 1.0 O1A B:AGS800 4.8 36.4 1.0 O B:HOH956 5.0 61.0 1.0 CD B:GLU305 5.0 35.7 1.0 C B:LYS251 5.0 30.4 1.0

W.K.Tang, D.Xia. Altered Intersubunit Communication Is the Molecular Basis For Functional Defects of Pathogenic P97 Mutants. J.Biol.Chem. V. 288 36624 2013.
ISSN: ISSN 0021-9258
PubMed: 24196964
DOI: 10.1074/JBC.M113.488924