Magnesium in PDB 4llg: Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex

All present enzymatic activity of Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex:
2.7.7.6;

The structure of Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex, PDB code: 4llg was solved by B.Bae, S.A.Darst, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.21 / 3.79
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	187.510, 205.038, 308.797, 90.00, 90.00, 90.00
R / Rfree (%)	24.3 / 28.2

The structure of Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Magnesium atom in the Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex (pdb code 4llg). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex, PDB code: 4llg:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1501 b:51.5 occ:1.00 OD1 D:ASP462 3.0 79.5 1.0 OD2 D:ASP462 3.3 77.4 1.0 OD1 D:ASP460 3.4 88.3 1.0 OD1 D:ASP464 3.5 0.2 1.0 CG D:ASP462 3.5 77.6 1.0 OD2 D:ASP464 3.6 77.3 1.0 CB D:ASP460 3.8 71.9 1.0 CG D:ASP460 4.0 0.8 1.0 CG D:ASP464 4.0 82.3 1.0 N D:ASP460 4.7 69.9 1.0 CA D:ASP460 4.8 66.7 1.0 NH2 D:ARG425 4.9 56.4 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure Analysis of the E.Coli Holoenzyme/GP2 Complex within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg1501 b:55.5 occ:1.00 OD1 J:ASP462 3.0 78.8 1.0 OD2 J:ASP462 3.3 64.4 1.0 OD1 J:ASP460 3.4 84.2 1.0 OD1 J:ASP464 3.5 0.3 1.0 CG J:ASP462 3.5 74.7 1.0 OD2 J:ASP464 3.6 91.2 1.0 CB J:ASP460 3.8 65.6 1.0 CG J:ASP460 4.0 98.5 1.0 CG J:ASP464 4.0 87.2 1.0 N J:ASP460 4.7 71.1 1.0 CA J:ASP460 4.8 66.1 1.0 NH2 J:ARG425 4.9 65.7 1.0

B.Bae, E.Davis, D.Brown, E.A.Campbell, S.Wigneshweraraj, S.A.Darst. Phage T7 GP2 Inhibition of Escherichia Coli Rna Polymerase Involves Misappropriation of Sigma 70 Domain 1.1. Proc.Natl.Acad.Sci.Usa V. 110 19772 2013.
ISSN: ISSN 0027-8424
PubMed: 24218560
DOI: 10.1073/PNAS.1314576110