Magnesium in PDB 4lpn: Frog M-Ferritin with Cobalt, D127E Mutant

All present enzymatic activity of Frog M-Ferritin with Cobalt, D127E Mutant:
1.16.3.1;

The structure of Frog M-Ferritin with Cobalt, D127E Mutant, PDB code: 4lpn was solved by R.Torres, R.Behera, C.W.Goulding, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.00 / 1.66
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	184.003, 184.003, 184.003, 90.00, 90.00, 90.00
R / Rfree (%)	15.9 / 17.7

The structure of Frog M-Ferritin with Cobalt, D127E Mutant also contains other interesting chemical elements:

Cobalt	(Co)	11 atoms
Chlorine	(Cl)	9 atoms

The binding sites of Magnesium atom in the Frog M-Ferritin with Cobalt, D127E Mutant (pdb code 4lpn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Frog M-Ferritin with Cobalt, D127E Mutant, PDB code: 4lpn:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Frog M-Ferritin with Cobalt, D127E Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Frog M-Ferritin with Cobalt, D127E Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg220 b:21.5 occ:1.00 O A:HOH441 2.0 26.0 1.0 OG A:SER10 2.1 19.3 1.0 O A:HOH367 2.1 26.1 1.0 O A:HOH377 2.1 21.7 1.0 O A:HOH443 2.1 28.5 1.0 O A:HOH348 2.1 17.7 1.0 CB A:SER10 3.1 16.9 1.0 HB2 A:SER10 3.2 20.3 1.0 HA A:SER10 3.5 15.5 1.0 CA A:SER10 3.8 12.9 1.0 HB3 A:SER10 3.9 20.3 1.0 O A:SER10 4.1 14.3 1.0 OE1 A:GLU13 4.1 16.2 1.0 O A:HOH335 4.2 22.5 1.0 O A:HOH437 4.3 31.2 1.0 C A:SER10 4.3 13.8 1.0 HB3 A:GLU13 4.5 17.0 1.0 O A:HOH331 4.5 21.0 1.0 CD A:GLU13 4.6 15.9 1.0 HB2 A:ALA14 4.9 16.3 1.0 HH22 A:ARG5 4.9 18.7 1.0

Magnesium binding site 2 out of 3 in the Frog M-Ferritin with Cobalt, D127E Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Frog M-Ferritin with Cobalt, D127E Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg221 b:18.8 occ:0.33 O A:HOH472 2.1 33.4 1.0 O A:HOH420 2.1 29.1 1.0 HG23 A:THR118 3.9 23.6 1.0 O A:HOH427 4.2 33.6 1.0 HG21 A:THR118 4.4 23.6 1.0 O A:HOH344 4.4 24.9 1.0 O A:HOH405 4.5 26.0 1.0 CG2 A:THR118 4.6 19.6 1.0 O A:HOH404 4.7 26.1 1.0 HG22 A:THR118 4.9 23.6 1.0

Magnesium binding site 3 out of 3 in the Frog M-Ferritin with Cobalt, D127E Mutant


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Frog M-Ferritin with Cobalt, D127E Mutant within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg223 b:35.3 occ:1.00 O A:HOH492 2.1 39.6 1.0 O A:HOH491 2.1 43.1 1.0 CE1 A:HIS45 3.7 18.9 0.5 NE2 A:HIS45 3.8 26.5 0.5 ND1 A:HIS45 3.9 35.8 0.5 HE1 A:HIS45 3.9 22.6 0.5 HE2 A:HIS45 4.1 31.8 0.5 CD2 A:HIS45 4.1 30.9 0.5 CG A:HIS45 4.2 19.6 0.5 O A:HOH493 4.2 39.8 1.0 HD1 A:HIS45 4.2 43.0 0.5 HB2 A:HIS45 4.4 18.5 0.5 HD2 A:HIS45 4.6 37.1 0.5 HD1 A:HIS45 4.7 29.0 0.5 ND1 A:HIS45 4.8 24.1 0.5 HB3 A:HIS45 5.0 20.2 0.5 HB2 A:HIS45 5.0 20.2 0.5

R.Torres, R.Behera, T.Takehiko, J.Bradley, C.W.Goulding, E.C.Theil. D127E Ion Channel Exit Modification in Ferritin Nanocages Entraps Fe(II) and Impairs Its Distribution to Diiron Catalytic Centers To Be Published.