Magnesium in PDB 4m9q: Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp

All present enzymatic activity of Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp:
3.6.5.2;

The structure of Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp, PDB code: 4m9q was solved by M.Miertzschke, C.Koerner, M.Spoerner, A.Wittinghofer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.83 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	48.050, 76.830, 172.920, 90.00, 90.00, 90.00
R / Rfree (%)	19 / 23.5

The binding sites of Magnesium atom in the Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp (pdb code 4m9q). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp, PDB code: 4m9q:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:38.7 occ:1.00 OG1 A:THR33 1.9 33.8 1.0 O1B A:GNP301 2.0 29.2 1.0 OG1 A:THR50 2.0 42.9 1.0 O1G A:GNP301 2.0 36.5 1.0 O A:HOH401 2.0 34.9 1.0 O A:HOH422 2.1 33.2 1.0 CB A:THR33 3.0 34.3 1.0 CB A:THR50 3.1 43.0 1.0 PB A:GNP301 3.4 29.9 1.0 PG A:GNP301 3.4 34.3 1.0 N A:THR50 3.7 47.2 1.0 N3B A:GNP301 3.8 32.7 1.0 N A:THR33 3.8 34.3 1.0 O2G A:GNP301 3.9 36.0 1.0 OD2 A:ASP69 3.9 41.2 1.0 CG2 A:THR33 3.9 33.9 1.0 CA A:THR33 4.0 33.9 1.0 CA A:THR50 4.0 45.9 1.0 OD1 A:ASP69 4.0 37.6 1.0 CG2 A:THR50 4.1 41.8 1.0 O1A A:GNP301 4.2 37.9 1.0 O2B A:GNP301 4.4 29.1 1.0 O3A A:GNP301 4.4 32.9 1.0 O A:THR48 4.4 46.5 1.0 CG A:ASP69 4.4 37.0 1.0 O3G A:GNP301 4.5 40.2 1.0 ND2 A:ASN54 4.6 48.6 1.0 C A:PRO49 4.7 47.7 1.0 PA A:GNP301 4.7 34.7 1.0 O2A A:GNP301 4.7 31.7 1.0 CE A:LYS32 5.0 32.9 1.0 CA A:PRO49 5.0 46.7 1.0 CB A:LYS32 5.0 28.9 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg302 b:35.1 occ:1.00 O B:HOH401 1.9 34.3 1.0 O1G B:GNP301 1.9 37.8 1.0 OG1 B:THR33 2.0 35.7 1.0 O B:HOH412 2.1 31.4 1.0 OG1 B:THR50 2.1 41.6 1.0 O1B B:GNP301 2.1 30.3 1.0 CB B:THR50 3.0 45.9 1.0 CB B:THR33 3.1 32.4 1.0 PG B:GNP301 3.3 36.9 1.0 PB B:GNP301 3.4 31.2 1.0 N3B B:GNP301 3.7 35.8 1.0 OD1 B:ASP69 3.8 39.5 1.0 N B:THR33 3.8 29.7 1.0 O2G B:GNP301 3.9 37.6 1.0 N B:THR50 3.9 49.1 1.0 OD2 B:ASP69 3.9 41.0 1.0 CG2 B:THR50 4.0 46.4 1.0 CA B:THR33 4.0 29.4 1.0 CA B:THR50 4.1 47.6 1.0 CG2 B:THR33 4.2 30.4 1.0 CG B:ASP69 4.2 40.3 1.0 O2B B:GNP301 4.3 30.9 1.0 O3G B:GNP301 4.5 38.3 1.0 O B:HOH417 4.5 45.7 1.0 O1A B:GNP301 4.5 35.5 1.0 O3A B:GNP301 4.6 31.0 1.0 CE B:LYS32 4.6 29.2 1.0 O B:THR48 4.7 44.8 1.0 CB B:LYS32 4.7 26.1 1.0 O B:LEU70 4.8 44.3 1.0 PA B:GNP301 4.8 31.4 1.0 O2A B:GNP301 4.8 33.5 1.0 C B:LYS32 4.9 27.6 1.0 C B:PRO49 4.9 49.6 1.0 ND2 B:ASN54 4.9 35.1 0.5

Magnesium binding site 3 out of 3 in the Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of C-Terminally Truncated ARL13B From Chlamydomonas Rheinhardtii Bound to Gppnhp within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg302 b:41.1 occ:1.00 O1G C:GNP301 1.9 42.2 1.0 O C:HOH406 1.9 44.2 1.0 O C:HOH401 2.0 30.8 1.0 OG1 C:THR33 2.0 40.4 1.0 OG1 C:THR50 2.1 40.6 1.0 O1B C:GNP301 2.1 42.6 1.0 CB C:THR33 3.1 41.5 1.0 CB C:THR50 3.1 41.2 1.0 PG C:GNP301 3.2 42.9 1.0 PB C:GNP301 3.2 41.6 1.0 N3B C:GNP301 3.5 42.8 1.0 O2G C:GNP301 3.8 39.6 1.0 N C:THR33 3.8 39.5 1.0 N C:THR50 3.9 48.0 1.0 OD1 C:ASP69 4.0 39.6 1.0 OD2 C:ASP69 4.0 43.1 1.0 CA C:THR33 4.0 39.5 1.0 O2B C:GNP301 4.1 40.2 1.0 CG2 C:THR33 4.1 41.3 1.0 CA C:THR50 4.1 45.6 1.0 CG2 C:THR50 4.2 38.0 1.0 O1A C:GNP301 4.3 48.2 1.0 O3A C:GNP301 4.4 45.7 1.0 O3G C:GNP301 4.4 45.6 1.0 CG C:ASP69 4.4 36.9 1.0 O2A C:GNP301 4.5 46.5 1.0 PA C:GNP301 4.6 47.4 1.0 O C:THR48 4.6 52.7 1.0 CE C:LYS32 4.7 34.4 1.0 CB C:LYS32 4.8 33.5 1.0 C C:PRO49 4.8 50.6 1.0 ND2 C:ASN54 4.9 43.3 1.0 C C:LYS32 4.9 37.1 1.0 O C:LEU70 5.0 38.6 1.0

M.Miertzschke, C.Koerner, M.Spoerner, A.Wittinghofer. Structural Insights Into the Small G-Protein ARL13B and Implications For Joubert Syndrome. Biochem.J. V. 457 301 2014.
ISSN: ISSN 0264-6021
PubMed: 24168557
DOI: 10.1042/BJ20131097