Magnesium in PDB 4mex: Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A

All present enzymatic activity of Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A:
2.7.7.6;

The structure of Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A, PDB code: 4mex was solved by Y.Feng, Y.Zhang, E.Arnold, R.H.Ebright, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.87 / 3.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	185.800, 208.188, 308.179, 90.00, 90.00, 90.00
R / Rfree (%)	28.6 / 32.5

The structure of Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Magnesium atom in the Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A (pdb code 4mex). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A, PDB code: 4mex:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg2000 b:0.9 occ:1.00 OD1 D:ASP460 2.1 0.2 1.0 OD1 D:ASP462 2.4 0.8 1.0 OD1 D:ASP464 2.6 0.8 1.0 CG D:ASP460 3.0 0.4 1.0 OD2 D:ASP462 3.0 0.5 1.0 CG D:ASP462 3.0 0.9 1.0 OD2 D:ASP460 3.1 0.6 1.0 CG D:ASP464 3.4 0.0 1.0 OD2 D:ASP464 3.4 0.3 1.0 CB D:ASP460 4.4 0.8 1.0 CB D:ASP462 4.5 83.7 1.0 O D:ASP460 4.6 0.7 1.0 N D:ASP460 4.7 0.7 1.0 C D:ASP460 4.8 0.2 1.0 CA D:ASP460 4.8 0.2 1.0 CB D:ASP464 4.8 91.2 1.0 N D:ASP462 4.9 53.6 1.0 NH2 D:ARG425 5.0 0.9 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Escherichia Coli Rna Polymerase in Complex with Salinamide A within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg2000 b:98.5 occ:1.00 OD1 J:ASP460 2.3 0.7 1.0 OD1 J:ASP462 2.4 0.9 1.0 OD1 J:ASP464 2.6 0.5 1.0 OD2 J:ASP462 2.9 0.0 1.0 CG J:ASP462 3.0 0.7 1.0 CG J:ASP460 3.1 0.7 1.0 OD2 J:ASP460 3.2 0.6 1.0 CG J:ASP464 3.5 0.3 1.0 OD2 J:ASP464 3.5 0.8 1.0 CB J:ASP462 4.5 0.4 1.0 CB J:ASP460 4.6 0.2 1.0 NH2 J:ARG425 4.8 93.8 1.0 O J:ASP460 4.8 84.2 1.0 N J:ASP460 4.8 73.7 1.0 CB J:ASP464 4.9 0.5 1.0 N J:ASP462 4.9 0.1 1.0 C J:ASP460 5.0 80.2 1.0

D.Degen, Y.Feng, Y.Zhang, K.Y.Ebright, Y.W.Ebright, M.Gigliotti, H.Vahedian-Movahed, S.Mandal, M.Talaue, N.Connell, E.Arnold, W.Fenical, R.H.Ebright. Transcription Inhibition By the Depsipeptide Antibiotic Salinamide A. Elife V. 3 02451 2014.
ISSN: ESSN 2050-084X
PubMed: 24843001
DOI: 10.7554/ELIFE.02451