Magnesium in PDB 4mey: Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme

All present enzymatic activity of Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme:
2.7.7.6;

The structure of Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme, PDB code: 4mey was solved by Y.Feng, Y.Zhang, E.Arnold, R.H.Ebright, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.92 / 3.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	186.390, 207.238, 308.302, 90.00, 90.00, 90.00
R / Rfree (%)	27.6 / 32.5

The structure of Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Magnesium atom in the Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme (pdb code 4mey). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme, PDB code: 4mey:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg2000 b:0.8 occ:1.00 OD1 D:ASP462 2.0 72.9 1.0 OD1 D:ASP460 2.1 0.8 1.0 OD1 D:ASP464 2.2 65.9 1.0 OD2 D:ASP462 2.6 91.1 1.0 CG D:ASP462 2.6 76.3 1.0 CG D:ASP464 3.0 64.8 1.0 CG D:ASP460 3.0 0.3 1.0 OD2 D:ASP464 3.1 68.3 1.0 OD2 D:ASP460 3.4 0.5 1.0 CB D:ASP462 4.1 63.9 1.0 O D:ASP460 4.4 0.2 1.0 CB D:ASP464 4.4 49.9 1.0 CB D:ASP460 4.4 0.1 1.0 N D:ASP462 4.6 76.1 1.0 N D:ASP460 4.6 0.1 1.0 C D:ASP460 4.6 0.7 1.0 CA D:ASP462 4.7 75.1 1.0 CA D:ASP460 4.8 0.3 1.0 N D:ASP464 4.8 61.5 1.0 NH2 D:ARG425 4.8 46.1 1.0 C D:ASP462 4.9 72.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Escherichia Coli Rna Polymerase Holoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg2000 b:0.9 occ:1.00 OD1 J:ASP464 2.1 99.9 1.0 OD1 J:ASP460 2.1 0.6 1.0 OD2 J:ASP462 2.2 0.7 1.0 OD1 J:ASP462 2.2 97.6 1.0 CG J:ASP462 2.4 0.0 1.0 CG J:ASP464 3.0 98.9 1.0 CG J:ASP460 3.0 0.2 1.0 OD2 J:ASP464 3.2 0.3 1.0 OD2 J:ASP460 3.4 0.3 1.0 CB J:ASP462 3.8 88.5 1.0 N J:ASP462 4.1 0.8 1.0 O J:ASP460 4.2 0.0 1.0 CB J:ASP460 4.3 0.9 1.0 CA J:ASP462 4.4 99.7 1.0 CB J:ASP464 4.4 83.9 1.0 C J:ASP460 4.4 0.5 1.0 N J:ASP464 4.6 95.6 1.0 C J:ASP462 4.6 97.4 1.0 CA J:ASP460 4.7 0.1 1.0 N J:ASP460 4.7 0.9 1.0 CA J:ASP464 4.9 85.8 1.0 N J:GLY463 4.9 42.5 1.0 N J:PHE461 5.0 53.4 1.0

D.Degen, Y.Feng, Y.Zhang, K.Y.Ebright, Y.W.Ebright, M.Gigliotti, H.Vahedian-Movahed, S.Mandal, M.Talaue, N.Connell, E.Arnold, W.Fenical, R.H.Ebright. Transcription Inhibition By the Depsipeptide Antibiotic Salinamide A. Elife V. 3 02451 2014.
ISSN: ESSN 2050-084X
PubMed: 24843001
DOI: 10.7554/ELIFE.02451