Atomistry » Magnesium » PDB 4mpo-4naa » 4n56
Atomistry »
  Magnesium »
    PDB 4mpo-4naa »
      4n56 »

Magnesium in PDB 4n56: Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna

Enzymatic activity of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna

All present enzymatic activity of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna:
2.7.7.7;

Protein crystallography data

The structure of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna, PDB code: 4n56 was solved by E.Y.Wu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.65 / 2.20
Space group P 31 2 1
Cell size a, b, c (Å), α, β, γ (°) 110.040, 110.040, 91.290, 90.00, 90.00, 120.00
R / Rfree (%) 22.3 / 27.3

Other elements in 4n56:

The structure of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna also contains other interesting chemical elements:

Manganese (Mn) 1 atom

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna (pdb code 4n56). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna, PDB code: 4n56:

Magnesium binding site 1 out of 1 in 4n56

Go back to Magnesium Binding Sites List in 4n56
Magnesium binding site 1 out of 1 in the Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg902

b:59.8
occ:1.00
OE1 A:GLU786 2.2 72.4 1.0
OD2 A:ASP610 2.5 72.7 1.0
CD A:GLU786 3.4 62.3 1.0
CG A:ASP610 3.7 56.6 1.0
OP1 B:DOC112 4.0 48.6 1.0
CG A:GLU786 4.2 62.7 1.0
OE2 A:GLU786 4.3 58.0 1.0
OD1 A:ASP610 4.4 61.6 1.0
CG A:GLU832 4.5 84.7 1.0
CB A:ASP610 4.8 54.5 1.0
P B:DOC112 5.0 47.9 1.0
C5' B:DOC112 5.0 44.7 1.0

Reference:

E.Y.Wu, A.R.Walsh, E.C.Materne, E.P.Hiltner, B.Zielinski, B.R.Miller, L.Mawby, E.Modeste, C.A.Parish, W.M.Barnes, M.B.Kermekchiev. A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KLENTAQ1 Dna Polymerase. Biochemistry V. 54 881 2015.
ISSN: ISSN 0006-2960
PubMed: 25537790
DOI: 10.1021/BI501198F
Page generated: Mon Aug 19 23:25:07 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy