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Magnesium in PDB 4n5s: Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp
Enzymatic activity of Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp
All present enzymatic activity of Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp:
2.7.7.7;
2.7.7.7;
Protein crystallography data
The structure of Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp, PDB code: 4n5s
was solved by
E.Y.Wu,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
| Resolution Low / High (Å) | 32.80 / 1.67 |
| Space group | P 31 2 1 |
| Cell size a, b, c (Å), α, β, γ (°) | 107.657, 107.657, 89.659, 90.00, 90.00, 120.00 |
| R / Rfree (%) | 16.1 / 19.2 |
Other elements in 4n5s:
The structure of Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp also contains other interesting chemical elements:
| Manganese | (Mn) | 6 atoms |
Magnesium Binding Sites:
The binding sites of Magnesium atom in the Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp
(pdb code 4n5s). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp, PDB code: 4n5s:
In total only one binding site of Magnesium was determined in the Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp, PDB code: 4n5s:
Magnesium binding site 1 out of 1 in 4n5s
Go back to
Magnesium Binding Sites List in 4n5s
Magnesium binding site 1 out
of 1 in the Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Ternary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna and Ddctp within 5.0Å range:
|
Reference:
E.Y.Wu,
A.R.Walsh,
E.C.Materne,
E.P.Hiltner,
B.Zielinski,
B.R.Miller,
L.Mawby,
E.Modeste,
C.A.Parish,
W.M.Barnes,
M.B.Kermekchiev.
A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KLENTAQ1 Dna Polymerase. Biochemistry V. 54 881 2015.
ISSN: ISSN 0006-2960
PubMed: 25537790
DOI: 10.1021/BI501198F
Page generated: Mon Aug 19 23:26:41 2024
ISSN: ISSN 0006-2960
PubMed: 25537790
DOI: 10.1021/BI501198F
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