Magnesium in PDB 4nm5: Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif

All present enzymatic activity of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif:
2.7.11.26;

The structure of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif, PDB code: 4nm5 was solved by J.L.Stamos, M.L.-H.Chu, M.D.Enos, N.Shah, W.I.Weis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.24 / 2.30
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	81.727, 81.727, 280.937, 90.00, 90.00, 120.00
R / Rfree (%)	18.3 / 23.2

The structure of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Magnesium atom in the Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif (pdb code 4nm5). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif, PDB code: 4nm5:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg406 b:83.3 occ:1.00 O1B A:ADP401 2.1 85.7 0.7 O1A A:ADP401 2.1 76.3 0.7 O A:HOH572 2.3 82.2 1.0 OD1 A:ASN186 2.3 52.8 1.0 OD2 A:ASP200 2.4 84.8 1.0 CG A:ASP200 3.2 64.7 1.0 CG A:ASN186 3.3 51.5 1.0 PB A:ADP401 3.4 84.5 0.7 PA A:ADP401 3.5 71.6 0.7 CB A:ASP200 3.6 48.8 1.0 ND2 A:ASN186 3.7 50.5 1.0 O3A A:ADP401 3.7 0.3 0.7 O2B A:ADP401 4.0 91.2 0.7 OD1 A:ASP200 4.3 67.0 1.0 O2A A:ADP401 4.4 53.7 0.7 O5' A:ADP401 4.4 78.4 0.7 O3' A:ADP401 4.5 71.5 0.7 O3B A:ADP401 4.5 85.6 0.7 CE A:LYS183 4.5 57.0 1.0 C5' A:ADP401 4.6 76.0 0.7 OD2 A:ASP181 4.6 58.7 1.0 CB A:ASN186 4.7 47.2 1.0 C3' A:ADP401 4.8 76.2 0.7 O A:GLN185 5.0 46.6 1.0 CA A:ASN186 5.0 49.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 C-Motif within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg407 b:89.5 occ:1.00 O2B A:ADP401 2.1 91.2 0.7 OD1 A:ASP200 2.4 67.0 1.0 O A:HOH537 3.1 63.2 1.0 CG A:ASP200 3.3 64.7 1.0 OD2 A:ASP200 3.4 84.8 1.0 PB A:ADP401 3.5 84.5 0.7 CA A:GLY202 3.6 58.8 1.0 NZ A:LYS85 3.8 76.7 1.0 N A:GLY202 3.8 57.5 1.0 O1B A:ADP401 4.0 85.7 0.7 O3B A:ADP401 4.1 85.6 0.7 C A:GLY202 4.3 63.4 1.0 O3A A:ADP401 4.6 0.3 0.7 CB A:ASP200 4.7 48.8 1.0 N A:SER203 4.7 50.0 1.0 CE A:LYS85 4.7 66.0 1.0 OE1 A:GLU97 4.9 57.8 1.0 O A:GLY202 4.9 60.2 1.0 OG A:SER203 5.0 64.2 1.0 OE2 A:GLU97 5.0 57.1 1.0

J.L.Stamos, M.L.Chu, M.D.Enos, N.Shah, W.I.Weis. Structural Basis of Gsk-3 Inhibition By N-Terminal Phosphorylation and By the Wnt Receptor LRP6. Elife V. 3 01998 2014.
ISSN: ESSN 2050-084X
PubMed: 24642411