Magnesium in PDB 4nm7: Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif

All present enzymatic activity of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif:
2.7.11.26;

The structure of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif, PDB code: 4nm7 was solved by J.L.Stamos, M.L.-H.Chu, M.D.Enos, N.Shah, W.I.Weis, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.35 / 2.30
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	82.002, 82.002, 280.345, 90.00, 90.00, 120.00
R / Rfree (%)	18.3 / 23.4

The structure of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Magnesium atom in the Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif (pdb code 4nm7). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif, PDB code: 4nm7:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg405 b:77.4 occ:1.00 O2B A:ADP401 2.3 58.0 0.7 OD1 A:ASP200 2.3 58.0 1.0 O A:HOH539 2.6 63.4 1.0 O A:HOH513 2.9 51.5 1.0 OD2 A:ASP200 3.0 63.4 1.0 CG A:ASP200 3.0 56.1 1.0 PB A:ADP401 3.7 58.2 0.7 CA A:GLY202 3.8 53.2 1.0 N A:GLY202 4.0 50.2 1.0 O1B A:ADP401 4.1 57.1 0.7 NZ A:LYS85 4.2 65.8 1.0 C A:GLY202 4.3 52.8 1.0 O3B A:ADP401 4.4 57.7 0.7 CB A:ASP200 4.5 44.7 1.0 N A:SER203 4.6 44.5 1.0 O3A A:ADP401 4.8 0.9 0.7 OG A:SER203 4.8 55.2 1.0 MG A:MG406 4.8 61.8 1.0 OD2 A:ASP181 4.8 56.1 1.0 O A:GLY202 4.9 53.3 1.0 O A:HOH571 4.9 65.1 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Gsk-3/Axin Complex Bound to Phosphorylated Wnt Receptor LRP6 E-Motif within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg406 b:61.8 occ:1.00 O1A A:ADP401 2.1 58.3 0.7 OD2 A:ASP200 2.2 63.4 1.0 O1B A:ADP401 2.2 57.1 0.7 O A:HOH582 2.2 67.4 1.0 O A:HOH571 2.3 65.1 1.0 OD1 A:ASN186 2.4 49.4 1.0 CG A:ASP200 3.2 56.1 1.0 PB A:ADP401 3.3 58.2 0.7 CG A:ASN186 3.3 46.9 1.0 PA A:ADP401 3.4 54.2 0.7 ND2 A:ASN186 3.6 46.9 1.0 CB A:ASP200 3.6 44.7 1.0 O A:HOH539 3.7 63.4 1.0 O3A A:ADP401 3.7 0.9 0.7 O2B A:ADP401 3.7 58.0 0.7 OD1 A:ASP200 4.2 58.0 1.0 O2A A:ADP401 4.4 43.5 0.7 O5' A:ADP401 4.4 57.7 0.7 OD2 A:ASP181 4.5 56.1 1.0 C5' A:ADP401 4.5 54.5 0.7 O3B A:ADP401 4.6 57.7 0.7 O3' A:ADP401 4.7 58.9 0.7 CB A:ASN186 4.7 40.8 1.0 CE A:LYS183 4.8 52.0 1.0 O A:HOH513 4.8 51.5 1.0 MG A:MG405 4.8 77.4 1.0 C3' A:ADP401 5.0 59.5 0.7

J.L.Stamos, M.L.Chu, M.D.Enos, N.Shah, W.I.Weis. Structural Basis of Gsk-3 Inhibition By N-Terminal Phosphorylation and By the Wnt Receptor LRP6. Elife V. 3 01998 2014.
ISSN: ESSN 2050-084X
PubMed: 24642411