Magnesium in PDB 4o25: Structure of Wild Type Mus Musculus Rheb Bound to Gtp

The structure of Structure of Wild Type Mus Musculus Rheb Bound to Gtp, PDB code: 4o25 was solved by M.T.Mazhab-Jafari, C.B.Marshall, J.Ho, N.Ishiyama, V.Stambolic, M.Ikura, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.90 / 2.20
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	57.746, 70.461, 79.771, 90.00, 90.00, 90.00
R / Rfree (%)	20.3 / 25.6

The binding sites of Magnesium atom in the Structure of Wild Type Mus Musculus Rheb Bound to Gtp (pdb code 4o25). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of Wild Type Mus Musculus Rheb Bound to Gtp, PDB code: 4o25:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structure of Wild Type Mus Musculus Rheb Bound to Gtp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Wild Type Mus Musculus Rheb Bound to Gtp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:35.3 occ:1.00 OG A:SER20 2.8 23.4 1.0 O3G A:GTP201 2.8 46.7 1.0 O A:HOH373 2.8 39.4 1.0 O A:THR61 2.9 24.6 1.0 O1B A:GTP201 3.0 25.3 1.0 OD1 A:ASP60 3.0 26.8 1.0 OD2 A:ASP60 3.3 29.9 1.0 CG A:ASP60 3.4 25.3 1.0 CE A:LYS19 3.5 16.5 1.0 O2G A:GTP201 3.6 27.6 1.0 PG A:GTP201 3.7 39.2 1.0 CB A:LYS19 3.9 18.0 1.0 PB A:GTP201 4.0 21.9 1.0 N A:SER20 4.0 18.0 1.0 CB A:SER20 4.1 17.5 1.0 C A:THR61 4.1 28.3 1.0 NZ A:LYS19 4.2 17.4 1.0 O2B A:GTP201 4.3 20.8 1.0 O3B A:GTP201 4.4 25.4 1.0 O A:PRO37 4.4 50.4 1.0 CA A:SER20 4.5 18.3 1.0 CD A:LYS19 4.5 14.2 1.0 C A:ASP60 4.5 21.3 1.0 N A:THR61 4.5 24.6 1.0 O A:ASP60 4.7 23.1 1.0 CB A:ASP60 4.7 25.7 1.0 C A:LYS19 4.7 18.9 1.0 CG A:LYS19 4.8 15.7 1.0 CA A:ALA62 4.9 28.9 1.0 N A:ALA62 4.9 28.7 1.0 CA A:LYS19 4.9 15.1 1.0 CA A:THR61 5.0 24.6 1.0 N A:GLY63 5.0 23.6 1.0

Magnesium binding site 2 out of 2 in the Structure of Wild Type Mus Musculus Rheb Bound to Gtp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Wild Type Mus Musculus Rheb Bound to Gtp within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg201 b:37.1 occ:1.00 O1B B:GTP202 2.8 24.0 1.0 O3G B:GTP202 2.8 34.5 1.0 OG1 B:THR38 2.9 25.2 1.0 O1A B:GTP202 2.9 28.3 1.0 OG B:SER20 2.9 20.1 1.0 O B:ASP36 2.9 33.5 1.0 CB B:SER20 3.4 19.6 1.0 O B:HOH309 3.5 30.9 1.0 O3B B:GTP202 3.5 33.4 1.0 PB B:GTP202 3.6 23.8 1.0 N B:THR38 3.7 32.6 1.0 PG B:GTP202 3.7 34.5 1.0 C B:ASP36 3.8 38.9 1.0 PA B:GTP202 3.9 26.5 1.0 CB B:THR38 3.9 26.9 1.0 O B:HOH305 4.1 25.6 1.0 CE1 B:TYR35 4.1 30.1 1.0 O3A B:GTP202 4.1 25.4 1.0 C B:PRO37 4.1 38.7 1.0 CA B:PRO37 4.1 35.9 1.0 O1G B:GTP202 4.3 36.3 1.0 CA B:THR38 4.3 33.8 1.0 N B:PRO37 4.3 38.5 1.0 O2A B:GTP202 4.3 30.8 1.0 CD1 B:TYR35 4.5 28.1 1.0 CA B:SER20 4.7 23.9 1.0 N B:ASP36 4.7 33.0 1.0 N B:SER20 4.8 20.9 1.0 CA B:ASP36 4.8 35.2 1.0 O2G B:GTP202 5.0 28.4 1.0

M.T.Mazhab-Jafari, C.B.Marshall, J.Ho, N.Ishiyama, V.Stambolic, M.Ikura. Structure-Guided Mutation of the Conserved G3-Box Glycine in Rheb Generates A Constitutively Activated Regulator of Mtor To Be Published.