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Magnesium in PDB 4oip: Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp

Enzymatic activity of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp

All present enzymatic activity of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp:
2.7.7.6;

Protein crystallography data

The structure of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp, PDB code: 4oip was solved by Y.Zhang, R.H.Ebright, E.Arnold, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.12 / 3.40
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 182.920, 104.391, 294.211, 90.00, 98.97, 90.00
R / Rfree (%) 20.8 / 24.6

Other elements in 4oip:

The structure of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp also contains other interesting chemical elements:

Zinc (Zn) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp (pdb code 4oip). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 6 binding sites of Magnesium where determined in the Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp, PDB code: 4oip:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6;

Magnesium binding site 1 out of 6 in 4oip

Go back to Magnesium Binding Sites List in 4oip
Magnesium binding site 1 out of 6 in the Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mg2001

b:93.9
occ:1.00
O B:ASP191 2.1 88.1 1.0
OD1 B:ASP183 2.1 90.9 1.0
OD1 B:ASP191 2.1 98.0 1.0
OD1 B:ASP193 2.1 81.9 1.0
C B:ASP191 3.2 89.1 1.0
CG B:ASP193 3.3 68.7 1.0
CG B:ASP183 3.4 85.0 1.0
CG B:ASP191 3.4 96.9 1.0
O B:LEU192 3.8 75.3 1.0
C B:LEU192 3.9 76.3 1.0
CA B:ASP191 4.0 91.4 1.0
OD2 B:ASP193 4.0 56.9 1.0
OD2 B:ASP183 4.1 83.4 1.0
N B:ASP193 4.1 68.4 1.0
CB B:ASP191 4.2 93.3 1.0
CA B:ASP193 4.2 62.1 1.0
N B:LEU192 4.2 83.5 1.0
OD2 B:ASP191 4.3 93.1 1.0
CG B:ARG30 4.3 66.7 1.0
CB B:ASP193 4.4 59.8 1.0
CB B:ASP183 4.4 74.1 1.0
CA B:ASP183 4.5 66.7 1.0
CA B:LEU192 4.5 78.6 1.0
CA B:ARG30 4.8 58.6 1.0
CB B:ARG30 4.8 64.5 1.0

Magnesium binding site 2 out of 6 in 4oip

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Magnesium binding site 2 out of 6 in the Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg2003

b:26.7
occ:1.00
OD1 D:ASP743 2.1 32.1 1.0
O D:HOH2101 2.1 48.0 1.0
O D:HOH2103 2.1 37.8 1.0
O D:HOH2102 2.1 32.1 1.0
OD1 D:ASP739 2.1 32.9 1.0
OD1 D:ASP741 2.1 29.9 1.0
OD2 D:ASP741 2.5 35.5 1.0
CG D:ASP741 2.6 29.6 1.0
CG D:ASP743 2.9 30.3 1.0
OD2 D:ASP743 3.0 32.0 1.0
CG D:ASP739 3.0 35.9 1.0
OD2 D:ASP739 3.4 47.2 1.0
O I:R2T4 3.9 35.3 1.0
CB D:ASP741 4.1 26.9 1.0
CB D:ASP743 4.3 24.3 1.0
CB I:R2T4 4.4 36.9 1.0
CB D:ASP739 4.4 29.3 1.0
O D:ASP739 4.6 31.8 1.0
N D:ASP743 4.6 21.2 1.0
N D:ASP739 4.6 38.3 1.0
C I:R2T4 4.7 39.3 1.0
CA I:R2T4 4.7 36.8 1.0
C D:ASP739 4.7 28.2 1.0
N D:ASP741 4.8 25.5 1.0
CA D:ASP739 4.8 29.4 1.0
NH2 D:ARG704 4.8 38.0 1.0
CA D:ASP743 4.8 25.7 1.0
CA D:ASP741 4.8 23.1 1.0
MG D:MG2004 5.0 69.6 1.0
C D:ASP741 5.0 23.8 1.0

Magnesium binding site 3 out of 6 in 4oip

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Magnesium binding site 3 out of 6 in the Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg2004

b:69.6
occ:1.00
OD2 D:ASP739 2.1 47.2 1.0
O2B D:ATP2007 2.2 0.5 1.0
O2G D:ATP2007 2.3 87.4 1.0
O D:HOH2101 3.3 48.0 1.0
CG D:ASP739 3.4 35.9 1.0
PB D:ATP2007 3.5 0.2 1.0
PG D:ATP2007 3.7 85.0 1.0
NH1 C:ARG879 4.0 55.4 1.0
O3B D:ATP2007 4.1 96.9 1.0
O1B D:ATP2007 4.1 0.8 1.0
CB D:ASP739 4.1 29.3 1.0
OD2 D:ASP741 4.3 35.5 1.0
OD1 D:ASP739 4.3 32.9 1.0
O3G D:ATP2007 4.5 75.2 1.0
OE1 I:R2T4 4.6 46.0 1.0
OD2 C:ASP686 4.6 43.0 1.0
O1G D:ATP2007 4.7 53.1 1.0
O3A D:ATP2007 4.8 0.3 1.0
O D:HOH2102 4.9 32.1 1.0
MG D:MG2003 5.0 26.7 1.0
OE1 C:GLU685 5.0 39.7 1.0

Magnesium binding site 4 out of 6 in 4oip

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Magnesium binding site 4 out of 6 in the Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg2005

b:33.6
occ:1.00
O D:LYS840 2.0 37.2 1.0
C D:LYS840 3.2 44.0 1.0
OD2 B:ASP168 3.6 40.4 1.0
OE1 B:GLU154 3.8 54.8 1.0
CA D:LYS840 4.1 45.9 1.0
O D:LEU839 4.1 43.8 1.0
N D:TYR841 4.1 50.0 1.0
CA D:TYR841 4.2 46.5 1.0
OD1 B:ASP168 4.3 50.0 1.0
CG B:ASP168 4.3 46.5 1.0
OE2 B:GLU64 4.4 82.2 1.0
OE1 B:GLU64 4.5 71.0 1.0
N D:VAL842 4.5 48.6 1.0
CB B:ALA153 4.8 59.6 1.0
C D:TYR841 4.9 44.6 1.0
CD B:GLU64 4.9 79.5 1.0
CD B:GLU154 4.9 65.5 1.0

Magnesium binding site 5 out of 6 in 4oip

Go back to Magnesium Binding Sites List in 4oip
Magnesium binding site 5 out of 6 in the Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mg2006

b:50.3
occ:1.00
O D:TRP897 2.3 49.2 1.0
O D:ILE900 3.1 55.2 1.0
C D:TRP897 3.4 50.4 1.0
O D:GLU898 3.7 60.2 1.0
CA D:GLU898 3.8 47.3 1.0
C D:GLU898 3.8 54.9 1.0
N D:GLU898 4.1 45.8 1.0
CD1 D:TRP897 4.2 52.2 1.0
NE1 D:TRP897 4.3 54.4 1.0
C D:ILE900 4.3 46.2 1.0
CG D:TRP897 4.5 53.9 1.0
CE2 D:TRP897 4.5 54.8 1.0
CA D:TRP897 4.6 55.0 1.0
N D:LEU899 4.7 53.0 1.0
CD2 D:TRP897 4.7 52.9 1.0
N D:ILE900 4.7 51.3 1.0

Magnesium binding site 6 out of 6 in 4oip

Go back to Magnesium Binding Sites List in 4oip
Magnesium binding site 6 out of 6 in the Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Crystal Structure of Thermus Thermophilus Transcription Initiation Complex Soaked with GE23077, Atp, and Cmpcpp within 5.0Å range:
probe atom residue distance (Å) B Occ
F:Mg2001

b:68.9
occ:1.00
O F:GLY296 2.2 67.8 1.0
O F:TRP299 2.3 60.7 1.0
O F:ALA292 2.3 76.7 1.0
C F:ALA292 3.3 71.3 1.0
C F:GLY296 3.3 70.1 1.0
C F:TRP299 3.4 60.4 1.0
CB F:TRP299 3.9 53.1 1.0
CA F:TRP299 3.9 58.6 1.0
N F:TRP299 4.0 57.2 1.0
N F:GLU293 4.0 77.1 1.0
CA F:GLU293 4.1 76.2 1.0
N F:GLY296 4.1 64.9 1.0
CA F:GLY296 4.1 69.7 1.0
CA F:ALA292 4.2 67.0 1.0
N F:PRO297 4.2 72.5 1.0
CB F:ALA292 4.3 69.0 1.0
CA F:PRO297 4.4 70.6 1.0
O F:GLU293 4.5 60.5 1.0
N F:ASP300 4.5 57.7 1.0
C F:GLU293 4.6 64.5 1.0
C F:PRO297 4.7 64.6 1.0
N F:GLY298 4.9 61.6 1.0
CA F:ASP300 4.9 58.9 1.0
C F:MET295 5.0 63.6 1.0

Reference:

Y.Zhang, D.Degen, M.X.Ho, E.Sineva, K.Y.Ebright, Y.W.Ebright, V.Mekler, H.Vahedian-Movahed, Y.Feng, R.Yin, S.Tuske, H.Irschik, R.Jansen, S.Maffioli, S.Donadio, E.Arnold, R.H.Ebright. GE23077 Binds to the Rna Polymerase 'I' and 'I+1' Sites and Prevents the Binding of Initiating Nucleotides. Elife V. 3 02450 2014.
ISSN: ESSN 2050-084X
PubMed: 24755292
Page generated: Mon Dec 14 19:17:55 2020

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