Magnesium in PDB 4ovn: Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
Protein crystallography data
The structure of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation, PDB code: 4ovn
was solved by
S.B.Gabelli,
M.A.Bianchet,
A.Boto,
J.Jakoncic,
G.F.Tomaselli,
L.M.Amzel,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
28.70 /
2.80
|
|
Space group
|
P 1 21 1
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
106.128,
99.006,
109.281,
90.00,
106.11,
90.00
|
|
R / Rfree (%)
|
21.1 /
28.2
|
Magnesium Binding Sites:
Pages:
>>> Page 1 <<<
Page 2, Binding sites: 11 -
18;
Binding sites:
The binding sites of Magnesium atom in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
(pdb code 4ovn). This binding sites where shown within
5.0 Angstroms radius around Magnesium atom.
In total 18 binding sites of Magnesium where determined in the
Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation, PDB code: 4ovn:
Jump to Magnesium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
10;
Magnesium binding site 1 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 1 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 1 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg201
b:90.0
occ:1.00
|
O
|
A:HOH304
|
2.0
|
0.7
|
1.0
|
|
O
|
A:THR27
|
2.1
|
0.8
|
1.0
|
|
OD1
|
A:ASP23
|
2.1
|
0.8
|
1.0
|
|
OD1
|
A:ASP21
|
2.1
|
0.1
|
1.0
|
|
OD1
|
A:ASP25
|
2.1
|
0.8
|
1.0
|
|
CG
|
A:ASP25
|
3.1
|
0.5
|
1.0
|
|
C
|
A:THR27
|
3.3
|
0.8
|
1.0
|
|
CG
|
A:ASP23
|
3.3
|
0.9
|
1.0
|
|
CG
|
A:ASP21
|
3.3
|
0.5
|
1.0
|
|
HA
|
A:ILE28
|
3.4
|
0.9
|
1.0
|
|
H
|
A:THR27
|
3.4
|
0.8
|
1.0
|
|
OD2
|
A:ASP25
|
3.4
|
0.6
|
1.0
|
|
HG1
|
A:THR27
|
3.5
|
0.4
|
1.0
|
|
H
|
A:ASP25
|
3.6
|
0.3
|
1.0
|
|
O
|
A:HOH308
|
3.8
|
80.5
|
1.0
|
|
OG1
|
A:THR27
|
3.9
|
0.2
|
1.0
|
|
HA
|
A:ASP21
|
3.9
|
0.4
|
1.0
|
|
HB2
|
A:ASP23
|
3.9
|
0.9
|
1.0
|
|
H
|
A:GLY24
|
4.0
|
0.2
|
1.0
|
|
HG23
|
A:THR29
|
4.1
|
0.2
|
1.0
|
|
N
|
A:THR27
|
4.1
|
0.2
|
1.0
|
|
OD2
|
A:ASP21
|
4.1
|
0.9
|
1.0
|
|
H
|
A:ASP23
|
4.1
|
1.0
|
1.0
|
|
OD2
|
A:ASP23
|
4.1
|
0.2
|
1.0
|
|
N
|
A:ILE28
|
4.2
|
0.0
|
1.0
|
|
CA
|
A:ILE28
|
4.2
|
0.6
|
1.0
|
|
CB
|
A:ASP23
|
4.2
|
0.7
|
1.0
|
|
CA
|
A:THR27
|
4.2
|
1.0
|
1.0
|
|
H
|
A:THR29
|
4.3
|
0.4
|
1.0
|
|
CB
|
A:ASP21
|
4.3
|
0.2
|
1.0
|
|
HB2
|
A:ASP21
|
4.4
|
97.9
|
1.0
|
|
N
|
A:ASP25
|
4.4
|
0.2
|
1.0
|
|
CB
|
A:ASP25
|
4.5
|
0.4
|
1.0
|
|
CA
|
A:ASP21
|
4.5
|
0.1
|
1.0
|
|
H
|
A:GLY26
|
4.6
|
0.6
|
1.0
|
|
HG22
|
A:ILE28
|
4.7
|
94.9
|
1.0
|
|
CB
|
A:THR27
|
4.7
|
0.8
|
1.0
|
|
N
|
A:GLY24
|
4.7
|
0.0
|
1.0
|
|
N
|
A:THR29
|
4.8
|
0.9
|
1.0
|
|
N
|
A:ASP23
|
4.8
|
0.5
|
1.0
|
|
N
|
A:GLY26
|
4.8
|
0.6
|
1.0
|
|
CA
|
A:ASP25
|
4.8
|
0.6
|
1.0
|
|
C
|
A:ILE28
|
4.9
|
0.8
|
1.0
|
|
HB3
|
A:ASP25
|
4.9
|
0.5
|
1.0
|
|
HB3
|
A:ASP23
|
5.0
|
0.9
|
1.0
|
|
C
|
A:ASP25
|
5.0
|
0.1
|
1.0
|
|
HG23
|
A:ILE28
|
5.0
|
94.9
|
1.0
|
|
Magnesium binding site 2 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 2 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 2 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg202
b:0.6
occ:1.00
|
O
|
A:HOH305
|
2.0
|
0.5
|
1.0
|
|
O
|
A:HOH306
|
2.1
|
91.9
|
1.0
|
|
OG1
|
A:THR63
|
2.1
|
1.0
|
1.0
|
|
O
|
A:THR63
|
2.1
|
0.7
|
1.0
|
|
OD1
|
A:ASP57
|
2.1
|
0.9
|
1.0
|
|
OE1
|
A:GLU68
|
2.3
|
0.3
|
1.0
|
|
C
|
A:THR63
|
2.9
|
0.1
|
1.0
|
|
CD
|
A:GLU68
|
3.2
|
1.0
|
1.0
|
|
CG
|
A:ASP57
|
3.2
|
0.1
|
1.0
|
|
CB
|
A:THR63
|
3.3
|
0.1
|
1.0
|
|
HA
|
A:ASP57
|
3.4
|
0.6
|
1.0
|
|
OE2
|
A:GLU68
|
3.4
|
0.7
|
1.0
|
|
CA
|
A:THR63
|
3.5
|
0.9
|
1.0
|
|
H
|
A:THR63
|
3.5
|
0.5
|
1.0
|
|
HA
|
A:ILE64
|
3.6
|
0.1
|
1.0
|
|
HB2
|
A:ASP57
|
3.7
|
0.5
|
1.0
|
|
HG12
|
A:VAL56
|
3.7
|
0.4
|
1.0
|
|
HB
|
A:THR63
|
3.7
|
0.9
|
1.0
|
|
CB
|
A:ASP57
|
3.8
|
0.8
|
1.0
|
|
N
|
A:THR63
|
3.9
|
0.7
|
1.0
|
|
N
|
A:ILE64
|
3.9
|
96.7
|
1.0
|
|
CA
|
A:ASP57
|
4.0
|
0.1
|
1.0
|
|
OD1
|
A:ASP59
|
4.1
|
0.6
|
1.0
|
|
OD2
|
A:ASP57
|
4.2
|
0.1
|
1.0
|
|
CA
|
A:ILE64
|
4.3
|
0.2
|
1.0
|
|
HA
|
A:THR63
|
4.4
|
0.2
|
1.0
|
|
HG23
|
A:THR63
|
4.4
|
0.5
|
1.0
|
|
CG2
|
A:THR63
|
4.4
|
0.9
|
1.0
|
|
O
|
A:VAL56
|
4.4
|
0.3
|
1.0
|
|
N
|
A:ASP57
|
4.5
|
1.0
|
1.0
|
|
HG12
|
A:ILE64
|
4.6
|
90.8
|
1.0
|
|
H
|
A:ASP65
|
4.6
|
1.0
|
1.0
|
|
CG
|
A:GLU68
|
4.6
|
0.5
|
1.0
|
|
HB2
|
A:ASN61
|
4.6
|
0.4
|
1.0
|
|
CG1
|
A:VAL56
|
4.6
|
0.8
|
1.0
|
|
HG21
|
A:THR63
|
4.7
|
0.5
|
1.0
|
|
C
|
A:VAL56
|
4.7
|
0.5
|
1.0
|
|
H
|
A:ILE64
|
4.7
|
0.6
|
1.0
|
|
HB3
|
A:ASP57
|
4.8
|
0.5
|
1.0
|
|
HG3
|
A:GLU68
|
4.8
|
0.5
|
1.0
|
|
HG11
|
A:VAL56
|
4.9
|
0.4
|
1.0
|
|
HB3
|
A:GLU68
|
4.9
|
0.1
|
1.0
|
|
HG13
|
A:VAL56
|
4.9
|
0.4
|
1.0
|
|
HD22
|
A:ASN61
|
5.0
|
0.1
|
1.0
|
|
H
|
A:ASP57
|
5.0
|
0.9
|
1.0
|
|
Magnesium binding site 3 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 3 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 3 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg203
b:66.5
occ:1.00
|
O
|
A:HOH302
|
1.9
|
68.5
|
1.0
|
|
O
|
A:TYR100
|
2.0
|
55.5
|
1.0
|
|
OD1
|
A:ASP96
|
2.1
|
61.9
|
1.0
|
|
OD1
|
A:ASN98
|
2.1
|
84.4
|
1.0
|
|
OD1
|
A:ASP94
|
2.1
|
70.6
|
1.0
|
|
HD21
|
A:ASN98
|
2.5
|
83.6
|
1.0
|
|
CG
|
A:ASN98
|
2.8
|
65.9
|
1.0
|
|
ND2
|
A:ASN98
|
3.0
|
62.0
|
1.0
|
|
CG
|
A:ASP96
|
3.1
|
64.0
|
1.0
|
|
H
|
A:TYR100
|
3.1
|
79.8
|
1.0
|
|
C
|
A:TYR100
|
3.2
|
55.4
|
1.0
|
|
CG
|
A:ASP94
|
3.3
|
66.6
|
1.0
|
|
OD2
|
A:ASP96
|
3.5
|
73.5
|
1.0
|
|
H
|
A:ASN98
|
3.6
|
79.8
|
1.0
|
|
HA
|
A:ASP94
|
3.7
|
91.3
|
1.0
|
|
H
|
A:GLY97
|
3.8
|
88.6
|
1.0
|
|
HD22
|
A:ASN98
|
3.8
|
83.6
|
1.0
|
|
N
|
A:TYR100
|
3.8
|
55.9
|
1.0
|
|
HA
|
A:ILE101
|
3.8
|
79.8
|
1.0
|
|
HB2
|
A:TYR100
|
3.8
|
80.3
|
1.0
|
|
HB2
|
A:ASP94
|
3.9
|
79.8
|
1.0
|
|
CA
|
A:TYR100
|
4.0
|
55.8
|
1.0
|
|
CB
|
A:ASP94
|
4.0
|
66.2
|
1.0
|
|
H
|
A:ASP96
|
4.0
|
79.8
|
1.0
|
|
HG13
|
A:ILE101
|
4.2
|
79.8
|
1.0
|
|
CA
|
A:ASP94
|
4.2
|
75.7
|
1.0
|
|
N
|
A:ILE101
|
4.2
|
55.2
|
1.0
|
|
CB
|
A:ASN98
|
4.3
|
59.4
|
1.0
|
|
OD2
|
A:ASP94
|
4.3
|
70.1
|
1.0
|
|
N
|
A:ASN98
|
4.3
|
59.1
|
1.0
|
|
CB
|
A:TYR100
|
4.3
|
56.2
|
1.0
|
|
H
|
A:GLY99
|
4.4
|
79.8
|
1.0
|
|
CB
|
A:ASP96
|
4.4
|
62.7
|
1.0
|
|
N
|
A:GLY97
|
4.5
|
67.7
|
1.0
|
|
HG12
|
A:ILE101
|
4.5
|
79.8
|
1.0
|
|
CA
|
A:ILE101
|
4.5
|
55.0
|
1.0
|
|
HB2
|
A:ASP96
|
4.5
|
79.8
|
1.0
|
|
HB3
|
A:TYR100
|
4.5
|
80.3
|
1.0
|
|
C
|
A:ASP94
|
4.6
|
84.8
|
1.0
|
|
N
|
A:ASP96
|
4.6
|
62.2
|
1.0
|
|
N
|
A:GLY99
|
4.7
|
60.5
|
1.0
|
|
HE21
|
A:GLN136
|
4.7
|
79.8
|
1.0
|
|
HB3
|
A:ASN98
|
4.7
|
79.8
|
1.0
|
|
CA
|
A:ASN98
|
4.8
|
59.4
|
1.0
|
|
CG1
|
A:ILE101
|
4.8
|
54.9
|
1.0
|
|
HB2
|
A:ASN98
|
4.8
|
79.8
|
1.0
|
|
H
|
A:LYS95
|
4.9
|
79.8
|
1.0
|
|
HA
|
A:TYR100
|
4.9
|
81.4
|
1.0
|
|
CA
|
A:ASP96
|
4.9
|
62.7
|
1.0
|
|
C
|
A:ASN98
|
4.9
|
59.1
|
1.0
|
|
HB3
|
A:ASP94
|
4.9
|
79.8
|
1.0
|
|
N
|
A:LYS95
|
4.9
|
60.6
|
1.0
|
|
C
|
A:GLY99
|
5.0
|
63.1
|
1.0
|
|
Magnesium binding site 4 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 4 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 4 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Mg204
b:70.5
occ:1.00
|
O
|
A:GLN136
|
2.0
|
85.3
|
1.0
|
|
O
|
A:HOH301
|
2.0
|
29.9
|
1.0
|
|
O
|
A:HOH303
|
2.0
|
0.3
|
1.0
|
|
OD1
|
A:ASP134
|
2.1
|
83.7
|
1.0
|
|
OD1
|
A:ASP130
|
2.1
|
84.4
|
1.0
|
|
OD1
|
A:ASP132
|
2.1
|
69.9
|
1.0
|
|
H
|
A:GLN136
|
2.5
|
84.5
|
1.0
|
|
CG
|
A:ASP132
|
2.9
|
69.1
|
1.0
|
|
OD2
|
A:ASP132
|
3.0
|
76.2
|
1.0
|
|
C
|
A:GLN136
|
3.1
|
61.7
|
1.0
|
|
CG
|
A:ASP130
|
3.2
|
73.4
|
1.0
|
|
CG
|
A:ASP134
|
3.2
|
70.4
|
1.0
|
|
H
|
A:ASP134
|
3.3
|
79.8
|
1.0
|
|
HA
|
A:ASP130
|
3.3
|
79.8
|
1.0
|
|
N
|
A:GLN136
|
3.3
|
66.0
|
1.0
|
|
HA
|
A:VAL137
|
3.4
|
79.8
|
1.0
|
|
HB2
|
A:ASP130
|
3.5
|
0.4
|
1.0
|
|
CB
|
A:ASP130
|
3.7
|
86.4
|
1.0
|
|
CA
|
A:GLN136
|
3.8
|
61.8
|
1.0
|
|
H
|
A:ASP132
|
3.8
|
0.1
|
1.0
|
|
OD2
|
A:ASP134
|
3.8
|
78.5
|
1.0
|
|
HB3
|
A:GLN136
|
3.8
|
79.8
|
1.0
|
|
H
|
A:GLY135
|
3.9
|
95.5
|
1.0
|
|
CA
|
A:ASP130
|
3.9
|
65.3
|
1.0
|
|
N
|
A:GLY135
|
4.0
|
75.4
|
1.0
|
|
N
|
A:ASP134
|
4.0
|
65.6
|
1.0
|
|
N
|
A:VAL137
|
4.0
|
60.8
|
1.0
|
|
CA
|
A:VAL137
|
4.1
|
60.9
|
1.0
|
|
C
|
A:ASP134
|
4.2
|
73.7
|
1.0
|
|
OD2
|
A:ASP130
|
4.3
|
80.8
|
1.0
|
|
CB
|
A:GLN136
|
4.3
|
62.1
|
1.0
|
|
C
|
A:GLY135
|
4.3
|
61.2
|
1.0
|
|
HB
|
A:VAL137
|
4.4
|
88.7
|
1.0
|
|
CB
|
A:ASP132
|
4.4
|
67.6
|
1.0
|
|
CB
|
A:ASP134
|
4.4
|
75.7
|
1.0
|
|
CA
|
A:ASP134
|
4.4
|
75.2
|
1.0
|
|
HA2
|
A:GLY135
|
4.4
|
90.9
|
1.0
|
|
CA
|
A:GLY135
|
4.5
|
71.0
|
1.0
|
|
C
|
A:ASP130
|
4.5
|
66.1
|
1.0
|
|
N
|
A:ASP132
|
4.6
|
88.0
|
1.0
|
|
HB2
|
A:GLN136
|
4.6
|
79.8
|
1.0
|
|
H
|
A:GLY133
|
4.7
|
92.1
|
1.0
|
|
H
|
A:ILE131
|
4.7
|
94.2
|
1.0
|
|
HA
|
A:GLN136
|
4.7
|
79.8
|
1.0
|
|
HB3
|
A:ASP130
|
4.7
|
0.4
|
1.0
|
|
HG12
|
A:VAL137
|
4.7
|
84.0
|
1.0
|
|
N
|
A:GLY133
|
4.8
|
76.8
|
1.0
|
|
O
|
A:ASP134
|
4.8
|
69.4
|
1.0
|
|
HB3
|
A:ASP134
|
4.8
|
91.9
|
1.0
|
|
CB
|
A:VAL137
|
4.8
|
68.1
|
1.0
|
|
HB2
|
A:ASP132
|
4.8
|
81.4
|
1.0
|
|
C
|
A:ASP132
|
4.8
|
80.6
|
1.0
|
|
CA
|
A:ASP132
|
4.8
|
67.9
|
1.0
|
|
N
|
A:ILE131
|
4.8
|
81.7
|
1.0
|
|
H
|
A:VAL137
|
4.8
|
79.8
|
1.0
|
|
HB3
|
A:ASP132
|
4.9
|
81.4
|
1.0
|
|
H
|
A:ASN138
|
5.0
|
79.8
|
1.0
|
|
Magnesium binding site 5 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 5 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 5 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg201
b:0.3
occ:1.00
|
OD1
|
B:ASP23
|
2.1
|
0.7
|
1.0
|
|
O
|
B:THR27
|
2.1
|
0.5
|
1.0
|
|
OD1
|
B:ASP25
|
2.1
|
0.4
|
1.0
|
|
OD1
|
B:ASP21
|
2.1
|
0.8
|
1.0
|
|
O
|
B:HOH302
|
2.1
|
0.6
|
1.0
|
|
CG
|
B:ASP25
|
2.9
|
0.9
|
1.0
|
|
OD2
|
B:ASP25
|
3.0
|
0.4
|
1.0
|
|
CG
|
B:ASP23
|
3.2
|
0.8
|
1.0
|
|
C
|
B:THR27
|
3.3
|
0.9
|
1.0
|
|
CG
|
B:ASP21
|
3.3
|
0.7
|
1.0
|
|
H
|
B:THR27
|
3.4
|
96.1
|
1.0
|
|
H
|
B:GLY24
|
3.5
|
0.6
|
1.0
|
|
H
|
B:GLY26
|
3.6
|
0.3
|
1.0
|
|
HA
|
B:ILE28
|
3.6
|
0.8
|
1.0
|
|
OD2
|
B:ASP23
|
3.7
|
0.3
|
1.0
|
|
H
|
B:ASP25
|
3.8
|
0.7
|
1.0
|
|
OD2
|
B:ASP21
|
3.9
|
0.6
|
1.0
|
|
N
|
B:THR27
|
3.9
|
0.1
|
1.0
|
|
HA
|
B:ASP21
|
4.1
|
0.9
|
1.0
|
|
CA
|
B:THR27
|
4.2
|
0.1
|
1.0
|
|
N
|
B:ASP25
|
4.2
|
0.2
|
1.0
|
|
HG23
|
B:ILE28
|
4.2
|
0.1
|
1.0
|
|
N
|
B:ILE28
|
4.2
|
0.0
|
1.0
|
|
CB
|
B:ASP25
|
4.3
|
0.1
|
1.0
|
|
N
|
B:GLY24
|
4.3
|
0.2
|
1.0
|
|
CA
|
B:ILE28
|
4.4
|
0.8
|
1.0
|
|
OG1
|
B:THR27
|
4.4
|
0.1
|
1.0
|
|
N
|
B:GLY26
|
4.4
|
0.9
|
1.0
|
|
HB2
|
B:ASP23
|
4.4
|
0.4
|
1.0
|
|
CB
|
B:ASP23
|
4.4
|
0.7
|
1.0
|
|
CB
|
B:ASP21
|
4.5
|
0.6
|
1.0
|
|
CA
|
B:ASP21
|
4.7
|
0.1
|
1.0
|
|
HB2
|
B:ASP21
|
4.8
|
0.7
|
1.0
|
|
CA
|
B:ASP25
|
4.8
|
0.1
|
1.0
|
|
HB3
|
B:ASP25
|
4.8
|
0.2
|
1.0
|
|
HG22
|
B:ILE28
|
4.8
|
0.1
|
1.0
|
|
HB2
|
B:ASP25
|
4.8
|
0.2
|
1.0
|
|
C
|
B:GLY24
|
4.8
|
0.7
|
1.0
|
|
H
|
B:ASP23
|
4.9
|
0.3
|
1.0
|
|
CG2
|
B:ILE28
|
4.9
|
0.6
|
1.0
|
|
CB
|
B:THR27
|
4.9
|
0.6
|
1.0
|
|
C
|
B:GLY26
|
5.0
|
0.0
|
1.0
|
|
CA
|
B:GLY24
|
5.0
|
0.9
|
1.0
|
|
HA
|
B:THR27
|
5.0
|
0.4
|
1.0
|
|
HA2
|
B:GLY24
|
5.0
|
0.0
|
1.0
|
|
Magnesium binding site 6 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 6 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 6 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg202
b:66.5
occ:1.00
|
OD1
|
B:ASN98
|
2.0
|
80.5
|
1.0
|
|
OD1
|
B:ASP94
|
2.0
|
66.8
|
1.0
|
|
O
|
B:TYR100
|
2.1
|
64.1
|
1.0
|
|
OD1
|
B:ASP96
|
2.1
|
64.2
|
1.0
|
|
HD21
|
B:ASN98
|
2.7
|
0.6
|
1.0
|
|
CG
|
B:ASN98
|
2.9
|
65.9
|
1.0
|
|
CG
|
B:ASP96
|
3.1
|
64.3
|
1.0
|
|
ND2
|
B:ASN98
|
3.1
|
93.8
|
1.0
|
|
H
|
B:TYR100
|
3.2
|
95.9
|
1.0
|
|
C
|
B:TYR100
|
3.3
|
64.3
|
1.0
|
|
CG
|
B:ASP94
|
3.3
|
66.9
|
1.0
|
|
H
|
B:ASN98
|
3.3
|
79.8
|
1.0
|
|
OD2
|
B:ASP96
|
3.3
|
72.6
|
1.0
|
|
H
|
B:GLY97
|
3.4
|
79.8
|
1.0
|
|
HA
|
B:ASP94
|
3.6
|
87.2
|
1.0
|
|
HA
|
B:ILE101
|
3.7
|
79.8
|
1.0
|
|
H
|
B:ASP96
|
3.8
|
89.3
|
1.0
|
|
N
|
B:TYR100
|
3.9
|
77.5
|
1.0
|
|
HD22
|
B:ASN98
|
4.0
|
0.6
|
1.0
|
|
OD2
|
B:ASP94
|
4.0
|
82.0
|
1.0
|
|
N
|
B:ASN98
|
4.0
|
64.4
|
1.0
|
|
HB2
|
B:TYR100
|
4.0
|
83.3
|
1.0
|
|
CA
|
B:TYR100
|
4.1
|
70.3
|
1.0
|
|
N
|
B:GLY97
|
4.2
|
64.5
|
1.0
|
|
CA
|
B:ASP94
|
4.2
|
73.0
|
1.0
|
|
N
|
B:ILE101
|
4.2
|
64.4
|
1.0
|
|
CB
|
B:ASP94
|
4.3
|
77.9
|
1.0
|
|
HG12
|
B:ILE101
|
4.3
|
83.5
|
1.0
|
|
CB
|
B:ASN98
|
4.3
|
64.5
|
1.0
|
|
C
|
B:ASP94
|
4.4
|
74.1
|
1.0
|
|
CA
|
B:ILE101
|
4.4
|
64.4
|
1.0
|
|
HG13
|
B:ILE101
|
4.4
|
83.5
|
1.0
|
|
CB
|
B:ASP96
|
4.4
|
64.6
|
1.0
|
|
H
|
B:GLY99
|
4.5
|
79.8
|
1.0
|
|
CB
|
B:TYR100
|
4.5
|
67.4
|
1.0
|
|
N
|
B:ASP96
|
4.5
|
68.5
|
1.0
|
|
HB2
|
B:ASP94
|
4.6
|
92.9
|
1.0
|
|
O
|
B:ASP94
|
4.6
|
67.2
|
1.0
|
|
CA
|
B:ASN98
|
4.6
|
64.5
|
1.0
|
|
HB3
|
B:TYR100
|
4.7
|
83.3
|
1.0
|
|
N
|
B:GLY99
|
4.7
|
64.4
|
1.0
|
|
H
|
B:LYS95
|
4.7
|
87.2
|
1.0
|
|
HB3
|
B:ASN98
|
4.8
|
79.8
|
1.0
|
|
CG1
|
B:ILE101
|
4.8
|
64.2
|
1.0
|
|
N
|
B:LYS95
|
4.8
|
70.9
|
1.0
|
|
HB2
|
B:ASP96
|
4.8
|
79.8
|
1.0
|
|
HB2
|
B:ASN98
|
4.8
|
79.8
|
1.0
|
|
CA
|
B:ASP96
|
4.9
|
64.7
|
1.0
|
|
C
|
B:GLY97
|
4.9
|
64.6
|
1.0
|
|
C
|
B:ASN98
|
4.9
|
64.5
|
1.0
|
|
HA
|
B:TYR100
|
5.0
|
87.1
|
1.0
|
|
CA
|
B:GLY97
|
5.0
|
64.7
|
1.0
|
|
C
|
B:ASP96
|
5.0
|
86.4
|
1.0
|
|
Magnesium binding site 7 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 7 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 7 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Mg203
b:95.8
occ:1.00
|
O
|
B:GLN136
|
2.0
|
87.5
|
1.0
|
|
O
|
B:HOH301
|
2.1
|
91.5
|
1.0
|
|
OD1
|
B:ASP132
|
2.1
|
0.1
|
1.0
|
|
OD1
|
B:ASP134
|
2.1
|
0.8
|
1.0
|
|
OD1
|
B:ASP130
|
2.1
|
0.1
|
1.0
|
|
H
|
B:GLN136
|
2.8
|
97.2
|
1.0
|
|
CG
|
B:ASP132
|
2.9
|
0.2
|
1.0
|
|
OD2
|
B:ASP132
|
3.1
|
0.8
|
1.0
|
|
H
|
B:GLY135
|
3.1
|
0.8
|
1.0
|
|
CG
|
B:ASP130
|
3.2
|
0.4
|
1.0
|
|
C
|
B:GLN136
|
3.2
|
83.5
|
1.0
|
|
CG
|
B:ASP134
|
3.2
|
0.3
|
1.0
|
|
HA
|
B:ASP130
|
3.3
|
0.6
|
1.0
|
|
HA
|
B:VAL137
|
3.4
|
79.8
|
1.0
|
|
N
|
B:GLN136
|
3.5
|
93.0
|
1.0
|
|
HB2
|
B:ASP130
|
3.5
|
96.4
|
1.0
|
|
H
|
B:GLY133
|
3.6
|
0.5
|
1.0
|
|
H
|
B:ASP134
|
3.7
|
88.9
|
1.0
|
|
OD2
|
B:ASP134
|
3.7
|
0.5
|
1.0
|
|
N
|
B:GLY135
|
3.8
|
0.8
|
1.0
|
|
CB
|
B:ASP130
|
3.8
|
94.7
|
1.0
|
|
H
|
B:ASP132
|
4.0
|
0.3
|
1.0
|
|
CA
|
B:ASP130
|
4.0
|
99.9
|
1.0
|
|
CA
|
B:GLN136
|
4.0
|
88.8
|
1.0
|
|
N
|
B:VAL137
|
4.1
|
70.2
|
1.0
|
|
CA
|
B:VAL137
|
4.2
|
68.0
|
1.0
|
|
N
|
B:ASP134
|
4.2
|
88.0
|
1.0
|
|
OD2
|
B:ASP130
|
4.3
|
0.1
|
1.0
|
|
HA2
|
B:GLY135
|
4.3
|
0.3
|
1.0
|
|
HB
|
B:VAL137
|
4.3
|
99.8
|
1.0
|
|
CA
|
B:GLY135
|
4.4
|
94.1
|
1.0
|
|
CB
|
B:ASP132
|
4.4
|
0.7
|
1.0
|
|
C
|
B:GLY135
|
4.4
|
87.6
|
1.0
|
|
N
|
B:GLY133
|
4.4
|
0.6
|
1.0
|
|
CB
|
B:ASP134
|
4.5
|
0.0
|
1.0
|
|
HB3
|
B:GLN136
|
4.5
|
79.8
|
1.0
|
|
C
|
B:ASP134
|
4.5
|
79.5
|
1.0
|
|
CA
|
B:ASP134
|
4.6
|
86.1
|
1.0
|
|
HB2
|
B:ASP132
|
4.6
|
0.8
|
1.0
|
|
HB3
|
B:ASP130
|
4.7
|
96.4
|
1.0
|
|
N
|
B:ASP132
|
4.7
|
0.4
|
1.0
|
|
CB
|
B:GLN136
|
4.8
|
77.6
|
1.0
|
|
HA
|
B:GLN136
|
4.8
|
93.2
|
1.0
|
|
CB
|
B:VAL137
|
4.8
|
84.8
|
1.0
|
|
H
|
B:ASN138
|
4.9
|
88.4
|
1.0
|
|
C
|
B:ASP130
|
4.9
|
0.7
|
1.0
|
|
HB3
|
B:ASP134
|
4.9
|
0.8
|
1.0
|
|
H
|
B:VAL137
|
4.9
|
82.7
|
1.0
|
|
CA
|
B:ASP132
|
5.0
|
0.4
|
1.0
|
|
HB2
|
B:GLN136
|
5.0
|
79.8
|
1.0
|
|
C
|
B:GLY133
|
5.0
|
0.9
|
1.0
|
|
Magnesium binding site 8 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 8 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 8 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg201
b:94.3
occ:1.00
|
O
|
C:HOH308
|
2.0
|
68.7
|
1.0
|
|
OD1
|
C:ASP25
|
2.1
|
0.3
|
1.0
|
|
O
|
C:THR27
|
2.1
|
79.5
|
1.0
|
|
OD1
|
C:ASP23
|
2.1
|
80.5
|
1.0
|
|
OD1
|
C:ASP21
|
2.1
|
0.6
|
1.0
|
|
CG
|
C:ASP23
|
3.1
|
0.1
|
1.0
|
|
H
|
C:THR27
|
3.2
|
86.5
|
1.0
|
|
CG
|
C:ASP25
|
3.2
|
91.5
|
1.0
|
|
HA
|
C:ASP21
|
3.2
|
89.8
|
1.0
|
|
C
|
C:THR27
|
3.3
|
82.0
|
1.0
|
|
CG
|
C:ASP21
|
3.3
|
0.2
|
1.0
|
|
OD2
|
C:ASP23
|
3.5
|
96.0
|
1.0
|
|
H
|
C:ASP23
|
3.5
|
0.2
|
1.0
|
|
H
|
C:ASP25
|
3.6
|
0.0
|
1.0
|
|
HB2
|
C:ASP21
|
3.6
|
96.2
|
1.0
|
|
OD2
|
C:ASP25
|
3.8
|
0.5
|
1.0
|
|
CB
|
C:ASP21
|
3.8
|
93.9
|
1.0
|
|
H
|
C:LYS22
|
3.9
|
98.0
|
1.0
|
|
CA
|
C:ASP21
|
3.9
|
88.4
|
1.0
|
|
N
|
C:THR27
|
3.9
|
82.0
|
1.0
|
|
HA
|
C:ILE28
|
3.9
|
86.5
|
1.0
|
|
OG1
|
C:THR27
|
4.0
|
86.9
|
1.0
|
|
CA
|
C:THR27
|
4.1
|
97.7
|
1.0
|
|
N
|
C:LYS22
|
4.2
|
99.6
|
1.0
|
|
N
|
C:ASP23
|
4.2
|
0.2
|
1.0
|
|
N
|
C:ASP25
|
4.2
|
0.8
|
1.0
|
|
C
|
C:ASP21
|
4.2
|
92.9
|
1.0
|
|
N
|
C:ILE28
|
4.3
|
65.5
|
1.0
|
|
CB
|
C:ASP25
|
4.4
|
88.8
|
1.0
|
|
OD2
|
C:ASP21
|
4.4
|
90.7
|
1.0
|
|
CB
|
C:ASP23
|
4.4
|
99.7
|
1.0
|
|
C
|
C:ASP25
|
4.5
|
89.8
|
1.0
|
|
H
|
C:GLY24
|
4.5
|
0.3
|
1.0
|
|
HG1
|
C:THR27
|
4.6
|
91.0
|
1.0
|
|
CA
|
C:ASP25
|
4.6
|
83.7
|
1.0
|
|
CA
|
C:ILE28
|
4.6
|
67.0
|
1.0
|
|
H
|
C:GLY26
|
4.6
|
82.6
|
1.0
|
|
HB3
|
C:ASP25
|
4.6
|
88.2
|
1.0
|
|
N
|
C:GLY26
|
4.7
|
75.3
|
1.0
|
|
CB
|
C:THR27
|
4.7
|
89.0
|
1.0
|
|
CA
|
C:ASP23
|
4.7
|
0.2
|
1.0
|
|
N
|
C:GLY24
|
4.7
|
0.6
|
1.0
|
|
HB3
|
C:ASP21
|
4.7
|
96.2
|
1.0
|
|
HG22
|
C:ILE28
|
4.8
|
86.0
|
1.0
|
|
O
|
C:ASP25
|
4.8
|
0.5
|
1.0
|
|
HB2
|
C:ASP23
|
4.8
|
0.8
|
1.0
|
|
C
|
C:ASP23
|
4.9
|
95.9
|
1.0
|
|
HA
|
C:THR27
|
5.0
|
0.9
|
1.0
|
|
Magnesium binding site 9 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 9 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 9 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg202
b:68.2
occ:1.00
|
O
|
C:HOH304
|
2.0
|
67.0
|
1.0
|
|
O
|
C:HOH301
|
2.0
|
93.2
|
1.0
|
|
O
|
C:THR63
|
2.1
|
0.3
|
1.0
|
|
O
|
C:HOH305
|
2.1
|
69.0
|
1.0
|
|
O
|
C:HOH302
|
2.1
|
96.7
|
1.0
|
|
OG1
|
C:THR63
|
2.1
|
90.1
|
1.0
|
|
C
|
C:THR63
|
3.1
|
88.0
|
1.0
|
|
CB
|
C:THR63
|
3.4
|
0.4
|
1.0
|
|
OE1
|
C:GLU68
|
3.5
|
71.6
|
1.0
|
|
HA
|
C:ILE64
|
3.7
|
79.8
|
1.0
|
|
O
|
C:VAL56
|
3.7
|
70.2
|
1.0
|
|
HB
|
C:THR63
|
3.7
|
0.2
|
1.0
|
|
CA
|
C:THR63
|
3.7
|
97.5
|
1.0
|
|
HG22
|
C:VAL56
|
3.7
|
82.3
|
1.0
|
|
N
|
C:THR63
|
4.1
|
0.8
|
1.0
|
|
OD2
|
C:ASP59
|
4.2
|
78.1
|
1.0
|
|
N
|
C:ILE64
|
4.2
|
80.2
|
1.0
|
|
OE2
|
C:GLU68
|
4.2
|
72.1
|
1.0
|
|
H
|
C:THR63
|
4.2
|
0.8
|
1.0
|
|
CD
|
C:GLU68
|
4.3
|
98.1
|
1.0
|
|
HA
|
C:ASP57
|
4.4
|
79.8
|
1.0
|
|
CA
|
C:ILE64
|
4.4
|
72.6
|
1.0
|
|
HG12
|
C:ILE64
|
4.5
|
98.9
|
1.0
|
|
CG2
|
C:THR63
|
4.6
|
95.2
|
1.0
|
|
OD1
|
C:ASP59
|
4.6
|
81.3
|
1.0
|
|
OD1
|
C:ASP57
|
4.6
|
89.9
|
1.0
|
|
HG23
|
C:THR63
|
4.6
|
92.2
|
1.0
|
|
HA
|
C:THR63
|
4.6
|
97.4
|
1.0
|
|
CG2
|
C:VAL56
|
4.7
|
67.9
|
1.0
|
|
HG21
|
C:THR63
|
4.7
|
92.2
|
1.0
|
|
C
|
C:VAL56
|
4.8
|
70.1
|
1.0
|
|
C
|
C:GLY62
|
4.8
|
83.6
|
1.0
|
|
CG
|
C:ASP59
|
4.8
|
83.1
|
1.0
|
|
H
|
C:ASP65
|
4.9
|
86.1
|
1.0
|
|
O
|
C:HOH310
|
4.9
|
66.5
|
1.0
|
|
HB2
|
C:ASN61
|
4.9
|
81.7
|
1.0
|
|
HG13
|
C:VAL56
|
4.9
|
84.3
|
1.0
|
|
HG21
|
C:VAL56
|
4.9
|
82.3
|
1.0
|
|
H
|
C:ILE64
|
5.0
|
88.2
|
1.0
|
|
Magnesium binding site 10 out
of 18 in 4ovn
Go back to
Magnesium Binding Sites List in 4ovn
Magnesium binding site 10 out
of 18 in the Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation
 Mono view
 Stereo pair view
|
|
A full contact list of Magnesium with other atoms in the Mg binding
site number 10 of Voltage-Gated Sodium Channel 1.5 (NAV1.5) C-Terminal Domain in Complex with Calmodulin Poised For Activation within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Mg203
b:80.9
occ:1.00
|
OD1
|
C:ASN98
|
2.0
|
90.4
|
1.0
|
|
O
|
C:TYR100
|
2.1
|
94.9
|
1.0
|
|
OD1
|
C:ASP96
|
2.1
|
0.4
|
1.0
|
|
OD1
|
C:ASP94
|
2.1
|
88.2
|
1.0
|
|
HD21
|
C:ASN98
|
2.6
|
0.9
|
1.0
|
|
CG
|
C:ASN98
|
2.8
|
0.4
|
1.0
|
|
ND2
|
C:ASN98
|
3.0
|
0.9
|
1.0
|
|
CG
|
C:ASP96
|
3.1
|
85.6
|
1.0
|
|
H
|
C:TYR100
|
3.2
|
83.3
|
1.0
|
|
C
|
C:TYR100
|
3.3
|
91.1
|
1.0
|
|
CG
|
C:ASP94
|
3.3
|
82.2
|
1.0
|
|
OD2
|
C:ASP96
|
3.5
|
92.0
|
1.0
|
|
H
|
C:ASN98
|
3.6
|
99.1
|
1.0
|
|
HA
|
C:ILE101
|
3.6
|
82.0
|
1.0
|
|
H
|
C:ASP96
|
3.8
|
90.8
|
1.0
|
|
HA
|
C:ASP94
|
3.8
|
95.3
|
1.0
|
|
HD22
|
C:ASN98
|
3.9
|
0.9
|
1.0
|
|
N
|
C:TYR100
|
3.9
|
75.5
|
1.0
|
|
HB2
|
C:TYR100
|
4.0
|
94.5
|
1.0
|
|
OD2
|
C:ASP94
|
4.0
|
76.5
|
1.0
|
|
CA
|
C:TYR100
|
4.1
|
80.9
|
1.0
|
|
HG12
|
C:ILE101
|
4.1
|
97.0
|
1.0
|
|
H
|
C:GLY99
|
4.2
|
0.5
|
1.0
|
|
N
|
C:ILE101
|
4.3
|
85.8
|
1.0
|
|
CB
|
C:ASN98
|
4.3
|
85.9
|
1.0
|
|
N
|
C:ASN98
|
4.3
|
92.9
|
1.0
|
|
CB
|
C:ASP94
|
4.4
|
79.3
|
1.0
|
|
CA
|
C:ILE101
|
4.4
|
71.2
|
1.0
|
|
HE21
|
C:GLN136
|
4.4
|
0.9
|
1.0
|
|
CA
|
C:ASP94
|
4.4
|
82.6
|
1.0
|
|
N
|
C:ASP96
|
4.4
|
80.5
|
1.0
|
|
O
|
C:HOH309
|
4.4
|
66.5
|
1.0
|
|
CB
|
C:ASP96
|
4.5
|
81.6
|
1.0
|
|
C
|
C:ASP94
|
4.5
|
78.5
|
1.0
|
|
CB
|
C:TYR100
|
4.5
|
86.5
|
1.0
|
|
N
|
C:GLY99
|
4.6
|
98.3
|
1.0
|
|
C
|
C:ASP96
|
4.6
|
77.2
|
1.0
|
|
H
|
C:LYS95
|
4.6
|
0.1
|
1.0
|
|
HB2
|
C:ASP94
|
4.6
|
90.4
|
1.0
|
|
CA
|
C:ASN98
|
4.7
|
84.8
|
1.0
|
|
CA
|
C:ASP96
|
4.7
|
76.8
|
1.0
|
|
N
|
C:LYS95
|
4.7
|
0.7
|
1.0
|
|
HB2
|
C:ASN98
|
4.7
|
88.3
|
1.0
|
|
HB3
|
C:ASN98
|
4.8
|
88.3
|
1.0
|
|
O
|
C:ASP96
|
4.8
|
95.0
|
1.0
|
|
HG23
|
C:ILE101
|
4.8
|
99.4
|
1.0
|
|
HB3
|
C:TYR100
|
4.8
|
94.5
|
1.0
|
|
N
|
C:GLY97
|
4.8
|
69.5
|
1.0
|
|
O
|
C:ASP94
|
4.8
|
79.9
|
1.0
|
|
H
|
C:GLY97
|
4.9
|
84.0
|
1.0
|
|
CG1
|
C:ILE101
|
4.9
|
82.1
|
1.0
|
|
HB3
|
C:ASP96
|
4.9
|
89.5
|
1.0
|
|
C
|
C:ASN98
|
5.0
|
0.0
|
1.0
|
|
HA
|
C:TYR100
|
5.0
|
89.5
|
1.0
|
|
Reference:
S.B.Gabelli,
A.Boto,
V.H.Kuhns,
M.A.Bianchet,
F.Farinelli,
S.Aripirala,
J.Yoder,
J.Jakoncic,
G.F.Tomaselli,
L.M.Amzel.
Regulation of the NAV1.5 Cytoplasmic Domain By Calmodulin. Nat Commun V. 5 5126 2014.
ISSN: ESSN 2041-1723
PubMed: 25370050
DOI: 10.1038/NCOMMS6126
Page generated: Tue Aug 20 01:00:10 2024
|
