Magnesium in PDB 4oyn: Fifteen Minutes Iron Loaded Human H Ferritin

All present enzymatic activity of Fifteen Minutes Iron Loaded Human H Ferritin:
1.16.3.1;

The structure of Fifteen Minutes Iron Loaded Human H Ferritin, PDB code: 4oyn was solved by C.Pozzi, F.Di Pisa, S.Mangani, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.09 / 1.43
Space group	F 4 3 2
Cell size a, b, c (Å), α, β, γ (°)	183.910, 183.910, 183.910, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 17.9

The structure of Fifteen Minutes Iron Loaded Human H Ferritin also contains other interesting chemical elements:

Iron	(Fe)	7 atoms
Chlorine	(Cl)	13 atoms

The binding sites of Magnesium atom in the Fifteen Minutes Iron Loaded Human H Ferritin (pdb code 4oyn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Fifteen Minutes Iron Loaded Human H Ferritin, PDB code: 4oyn:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Fifteen Minutes Iron Loaded Human H Ferritin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Fifteen Minutes Iron Loaded Human H Ferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg223 b:17.5 occ:0.70 O A:HOH335 2.1 18.6 0.7 O A:HOH366 2.1 26.0 0.7 O1 A:TRS201 2.3 23.0 0.7 O A:HOH553 2.4 29.5 0.7 O A:HOH357 2.4 33.2 0.7 C1 A:TRS201 2.9 23.2 0.7 N A:TRS201 3.2 24.4 0.7 C A:TRS201 3.7 23.8 0.7 OD1 A:ASP131 4.1 14.7 1.0 CG2 A:THR135 4.3 14.4 1.0 OD2 A:ASP131 4.4 14.1 1.0 O2 A:TRS201 4.4 24.9 0.7 OG1 A:THR135 4.6 14.3 1.0 C2 A:TRS201 4.7 24.2 0.7 CG A:ASP131 4.7 13.4 1.0 C3 A:TRS201 4.8 23.3 0.7 O A:HOH422 4.9 28.2 0.3 CB A:THR135 5.0 13.7 1.0

Magnesium binding site 2 out of 3 in the Fifteen Minutes Iron Loaded Human H Ferritin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Fifteen Minutes Iron Loaded Human H Ferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg224 b:13.6 occ:0.60 O A:HOH602 1.9 15.5 0.6 O A:HOH544 2.1 20.1 0.6 O A:HOH543 2.1 18.4 0.6 O A:HOH607 2.2 21.7 0.6 O A:HOH456 2.2 19.7 0.6 O A:HOH446 2.3 9.3 0.6 OD1 A:ASN25 4.2 8.2 1.0 O A:ARG22 4.4 8.9 1.0 CL A:CL222 4.4 22.9 0.7 O A:HOH508 4.4 26.6 1.0 O A:HOH490 4.6 20.8 1.0 O A:HOH481 4.8 24.1 1.0 O A:HOH598 4.9 43.6 1.0 CB A:LEU26 4.9 8.5 1.0 O A:HOH590 4.9 34.1 1.0 CB A:ASN25 5.0 8.0 1.0 CG A:ASN25 5.0 8.0 1.0

Magnesium binding site 3 out of 3 in the Fifteen Minutes Iron Loaded Human H Ferritin


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Fifteen Minutes Iron Loaded Human H Ferritin within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg225 b:10.6 occ:0.45 O A:HOH313 2.0 12.1 0.9 O A:HOH305 2.0 14.8 0.5 OD2 A:ASP84 4.0 10.1 1.0 OD1 A:ASP84 4.1 9.3 1.0 CG A:ASP84 4.5 9.5 1.0 CL A:CL212 4.7 15.9 0.7

C.Pozzi, F.Di Pisa, C.Bernacchioni, S.Ciambellotti, P.Turano, S.Mangani. Iron Binding to Human Heavy-Chain Ferritin. Acta Crystallogr.,Sect.D V. 71 1909 2015.
ISSN: ESSN 1399-0047
PubMed: 26327381
DOI: 10.1107/S1399004715013073