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Magnesium in PDB 4pha: Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp

Enzymatic activity of Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp

All present enzymatic activity of Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp:
2.7.7.7;

Protein crystallography data

The structure of Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp, PDB code: 4pha was solved by M.C.Koag, S.Lee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.52
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 54.440, 80.710, 54.834, 90.00, 108.88, 90.00
R / Rfree (%) 21.6 / 27.8

Other elements in 4pha:

The structure of Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp also contains other interesting chemical elements:

Sodium (Na) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp (pdb code 4pha). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp, PDB code: 4pha:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 4pha

Go back to Magnesium Binding Sites List in 4pha
Magnesium binding site 1 out of 2 in the Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg401

b:29.4
occ:1.00
OD1 A:ASP190 2.1 22.4 1.0
O A:HOH523 2.1 27.8 1.0
O2B A:0KX405 2.2 32.8 1.0
O1G A:0KX405 2.3 39.0 1.0
OD1 A:ASP192 2.4 25.1 1.0
O1A A:0KX405 2.6 26.2 1.0
CG A:ASP190 3.3 23.7 1.0
PB A:0KX405 3.3 30.2 1.0
PG A:0KX405 3.6 26.5 1.0
CG A:ASP192 3.6 30.0 1.0
PA A:0KX405 3.7 31.4 1.0
O3B A:0KX405 3.7 28.3 1.0
MG A:MG402 3.8 33.8 1.0
OD2 A:ASP190 3.8 24.8 1.0
O A:ASP190 3.8 24.7 1.0
N3A A:0KX405 3.9 36.3 1.0
O A:HOH518 3.9 24.4 1.0
CA A:GLY179 4.1 34.5 1.0
N A:SER180 4.1 32.6 1.0
C A:ASP190 4.1 22.2 1.0
OG A:SER180 4.2 23.4 1.0
O2G A:0KX405 4.2 33.4 1.0
O3G A:0KX405 4.3 27.1 1.0
N A:ASP190 4.4 27.0 1.0
O1B A:0KX405 4.4 32.4 1.0
CB A:ASP192 4.4 25.7 1.0
OD2 A:ASP192 4.5 35.3 1.0
CB A:ASP190 4.5 19.4 1.0
N A:ASP192 4.5 22.5 1.0
CA A:ASP190 4.6 23.6 1.0
C5' A:0KX405 4.6 32.5 1.0
C A:GLY179 4.6 28.7 1.0
O2A A:0KX405 4.7 27.2 1.0
N A:MET191 4.7 21.4 1.0
O5' A:0KX405 4.7 33.4 1.0
C A:MET191 4.9 23.6 1.0
CA A:MET191 4.9 23.2 1.0

Magnesium binding site 2 out of 2 in 4pha

Go back to Magnesium Binding Sites List in 4pha
Magnesium binding site 2 out of 2 in the Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Human Dna Polymerase Beta Complexed with A in the Template Base Paired with Incoming Non-Hydrolyzable Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg402

b:33.8
occ:1.00
O1A A:0KX405 2.4 26.2 1.0
OD2 A:ASP190 2.6 24.8 1.0
OD1 A:ASP192 2.7 25.1 1.0
OD2 A:ASP192 2.7 35.3 1.0
O3' P:DA10 3.0 43.1 1.0
CG A:ASP190 3.0 23.7 1.0
OD1 A:ASP190 3.0 22.4 1.0
CG A:ASP192 3.0 30.0 1.0
PA A:0KX405 3.4 31.4 1.0
OD2 A:ASP256 3.6 32.8 1.0
O2A A:0KX405 3.6 27.2 1.0
MG A:MG401 3.8 29.4 1.0
O5' A:0KX405 3.9 33.4 1.0
C3' P:DA10 3.9 34.5 1.0
C5' A:0KX405 4.2 32.5 1.0
C5' P:DA10 4.2 32.6 1.0
CB A:ASP190 4.3 19.4 1.0
C4' P:DA10 4.5 36.7 1.0
CG A:ASP256 4.5 20.2 1.0
CB A:ASP192 4.5 25.7 1.0
O2B A:0KX405 4.7 32.8 1.0
O5' P:DA10 4.8 24.7 1.0
O A:HOH514 4.8 14.5 1.0
CB A:ASP256 4.9 22.9 1.0
O A:MET191 4.9 23.8 1.0
N3A A:0KX405 4.9 36.3 1.0
O1G A:0KX405 5.0 39.0 1.0
O A:HOH539 5.0 27.9 1.0

Reference:

M.C.Koag, K.Nam, S.Lee. The Spontaneous Replication Error and the Mismatch Discrimination Mechanisms of Human Dna Polymerase Beta. Nucleic Acids Res. V. 42 11233 2015.
ISSN: ESSN 1362-4962
PubMed: 25200079
DOI: 10.1093/NAR/GKU789
Page generated: Tue Aug 20 01:20:51 2024

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