Magnesium in PDB 4pjk: Dicty Myosin II R238E.E459R Mutant (with Adp.Pi) in the Pi Release State

All present enzymatic activity of Dicty Myosin II R238E.E459R Mutant (with Adp.Pi) in the Pi Release State:
3.6.4.1;

The structure of Dicty Myosin II R238E.E459R Mutant (with Adp.Pi) in the Pi Release State, PDB code: 4pjk was solved by T.Isabet, H.Benisty, P.Llinas, H.L.Sweeney, A.Houdusse, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.55 / 2.15
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	87.100, 150.150, 154.040, 90.00, 90.00, 90.00
R / Rfree (%)	17.6 / 21.3

The binding sites of Magnesium atom in the Dicty Myosin II R238E.E459R Mutant (with Adp.Pi) in the Pi Release State (pdb code 4pjk). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Dicty Myosin II R238E.E459R Mutant (with Adp.Pi) in the Pi Release State, PDB code: 4pjk:

Magnesium binding site 1 out of 1 in the Dicty Myosin II R238E.E459R Mutant (with Adp.Pi) in the Pi Release State


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Dicty Myosin II R238E.E459R Mutant (with Adp.Pi) in the Pi Release State within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg801 b:37.0 occ:1.00 O A:HOH959 2.0 34.4 1.0 O A:HOH958 2.0 33.2 1.0 OG1 A:THR186 2.0 39.8 1.0 O2B A:ADP802 2.0 35.1 1.0 OG A:SER237 2.1 39.2 1.0 O A:HOH957 2.1 32.6 1.0 CB A:THR186 3.0 38.0 1.0 CB A:SER237 3.2 35.9 1.0 PB A:ADP802 3.3 37.6 1.0 O3B A:ADP802 3.5 36.0 1.0 N A:SER237 3.8 37.0 1.0 N A:THR186 3.9 32.3 1.0 O2A A:ADP802 4.0 37.8 1.0 CA A:THR186 4.0 33.2 1.0 CG2 A:THR186 4.0 37.0 1.0 CA A:SER237 4.1 36.5 1.0 O1 A:PO4803 4.1 39.5 0.6 O3A A:ADP802 4.2 33.4 1.0 OD2 A:ASP454 4.3 47.2 1.0 OD1 A:ASP454 4.3 46.9 1.0 O4 A:PO4803 4.4 40.7 0.6 O1B A:ADP802 4.5 37.2 1.0 O A:HOH964 4.5 46.8 1.0 PA A:ADP802 4.5 36.1 1.0 O1A A:ADP802 4.6 37.6 1.0 O A:ASN235 4.6 37.0 1.0 CG A:ASP454 4.7 47.2 1.0 ND2 A:ASN233 4.7 31.0 1.0 CB A:LYS185 4.9 31.3 1.0 C A:SER236 4.9 38.7 1.0 P A:PO4803 4.9 22.7 0.6 C A:LYS185 4.9 36.7 1.0 CE A:LYS185 5.0 41.0 1.0

P.Llinas, T.Isabet, L.Song, V.Ropars, B.Zong, H.Benisty, S.Sirigu, C.Morris, C.Kikuti, D.Safer, H.L.Sweeney, A.Houdusse. How Actin Initiates the Motor Activity of Myosin Dev.Cell V. 33 1 2015.
ISSN: ISSN 1534-5807
DOI: 10.1016/J.BPJ.2014.11.160