Magnesium in PDB 4rgf: Crystal Structure of the in-Line Aligned ENV22 Twister Ribozyme Soaked with MN2+

The structure of Crystal Structure of the in-Line Aligned ENV22 Twister Ribozyme Soaked with MN2+, PDB code: 4rgf was solved by A.Ren, K.R.Rajashankar, D.Simanshu, D.Patel, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.75 / 3.20
Space group	P 61 2 2
Cell size a, b, c (Å), α, β, γ (°)	64.299, 64.299, 584.991, 90.00, 90.00, 120.00
R / Rfree (%)	23.1 / 27.7

The structure of Crystal Structure of the in-Line Aligned ENV22 Twister Ribozyme Soaked with MN2+ also contains other interesting chemical elements:

Potassium	(K)	14 atoms
Manganese	(Mn)	11 atoms

The binding sites of Magnesium atom in the Crystal Structure of the in-Line Aligned ENV22 Twister Ribozyme Soaked with MN2+ (pdb code 4rgf). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Crystal Structure of the in-Line Aligned ENV22 Twister Ribozyme Soaked with MN2+, PDB code: 4rgf:

Magnesium binding site 1 out of 1 in the Crystal Structure of the in-Line Aligned ENV22 Twister Ribozyme Soaked with MN2+


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the in-Line Aligned ENV22 Twister Ribozyme Soaked with MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg101 b:0.0 occ:1.00 OP1 C:A6 2.2 0.9 1.0 O4 C:U8 2.5 0.8 1.0 OP2 C:A7 2.5 0.3 1.0 C4 C:U8 3.5 0.6 1.0 P C:A6 3.5 0.6 1.0 P C:A7 3.8 0.5 1.0 C5 C:U8 4.0 0.0 1.0 O6 C:G9 4.1 0.8 1.0 OP2 C:A6 4.1 0.8 1.0 OP1 C:A7 4.3 0.7 1.0 O5' C:A6 4.5 0.2 1.0 O3' C:A6 4.5 0.5 1.0 N6 C:A47 4.5 0.5 1.0 O4 C:U45 4.5 0.7 1.0 O3' C:DU5 4.6 0.7 1.0 N3 C:U8 4.7 0.4 1.0 N4 C:C46 4.9 1.0 1.0 C2' C:DU5 4.9 0.8 1.0 C3' C:A6 4.9 0.3 1.0 O5' C:A7 5.0 0.0 1.0

A.Ren, M.Kosutic, K.R.Rajashankar, M.Frener, T.Santner, E.Westhof, R.Micura, D.J.Patel. In-Line Alignment and Mg(2+) Coordination at the Cleavage Site of the ENV22 Twister Ribozyme. Nat Commun V. 5 5534 2014.
ISSN: ESSN 2041-1723
PubMed: 25410397
DOI: 10.1038/NCOMMS6534