Magnesium in PDB 4rnh: Pamora Tandem Diguanylate Cyclase - Phosphodiesterase, C-Di-Gmp Complex

All present enzymatic activity of Pamora Tandem Diguanylate Cyclase - Phosphodiesterase, C-Di-Gmp Complex:
2.7.7.65; 3.1.4.52;

The structure of Pamora Tandem Diguanylate Cyclase - Phosphodiesterase, C-Di-Gmp Complex, PDB code: 4rnh was solved by C.W.Phippen, I.Tews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	61.70 / 2.45
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	81.300, 81.300, 128.100, 90.00, 90.00, 120.00
R / Rfree (%)	18.4 / 24.3

The binding sites of Magnesium atom in the Pamora Tandem Diguanylate Cyclase - Phosphodiesterase, C-Di-Gmp Complex (pdb code 4rnh). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Pamora Tandem Diguanylate Cyclase - Phosphodiesterase, C-Di-Gmp Complex, PDB code: 4rnh:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Pamora Tandem Diguanylate Cyclase - Phosphodiesterase, C-Di-Gmp Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Pamora Tandem Diguanylate Cyclase - Phosphodiesterase, C-Di-Gmp Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1501 b:42.5 occ:1.00 OE1 A:GLU1280 2.0 57.8 1.0 O2P A:C2E1503 2.1 56.1 1.0 O A:HOH1619 2.3 45.8 1.0 OD2 A:ASP1310 2.3 42.0 1.0 OD1 A:ASN1248 2.4 41.0 1.0 OE2 A:GLU1189 2.5 44.8 1.0 CG A:ASP1310 3.0 42.6 1.0 CD A:GLU1280 3.0 49.4 1.0 P1 A:C2E1503 3.2 58.4 1.0 OD1 A:ASP1310 3.3 40.5 1.0 CD A:GLU1189 3.3 43.3 1.0 CG A:ASN1248 3.4 41.9 1.0 OE1 A:GLU1189 3.6 47.4 1.0 OE2 A:GLU1280 3.6 54.9 1.0 O1P A:C2E1503 3.6 59.0 1.0 ND2 A:ASN1248 3.8 44.9 1.0 CE A:LYS1331 3.9 46.7 1.0 O5' A:C2E1503 3.9 53.8 1.0 CG A:GLU1280 4.1 47.0 1.0 CB A:GLU1280 4.1 40.2 1.0 O A:HOH1610 4.2 40.5 1.0 CB A:ASP1310 4.2 42.2 1.0 O A:HOH1615 4.5 45.6 1.0 O3A A:C2E1503 4.5 54.6 1.0 CG A:GLU1189 4.5 42.9 1.0 NZ A:LYS1331 4.5 46.0 1.0 OE1 A:GLU1367 4.7 77.1 1.0 CD A:LYS1331 4.7 45.6 1.0 CB A:ASN1248 4.8 41.2 1.0 C5' A:C2E1503 4.9 53.1 1.0

Magnesium binding site 2 out of 2 in the Pamora Tandem Diguanylate Cyclase - Phosphodiesterase, C-Di-Gmp Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Pamora Tandem Diguanylate Cyclase - Phosphodiesterase, C-Di-Gmp Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1502 b:75.0 occ:1.00 O1P A:C2E1503 2.3 59.0 1.0 OD1 A:ASP1311 2.5 44.6 1.0 O A:HOH1615 2.9 45.6 1.0 O3A A:C2E1503 3.0 54.6 1.0 OD2 A:ASP1311 3.1 52.9 1.0 CG A:ASP1311 3.1 47.2 1.0 P1 A:C2E1503 3.2 58.4 1.0 NH1 A:ARG1334 3.8 96.4 1.0 OE2 A:GLU1367 3.9 74.3 1.0 OD1 A:ASP1333 4.3 45.0 1.0 O5' A:C2E1503 4.4 53.8 1.0 C3A A:C2E1503 4.4 56.0 1.0 O2P A:C2E1503 4.4 56.1 1.0 O2A A:C2E1503 4.5 51.5 1.0 OD1 A:ASP1310 4.6 40.5 1.0 C5' A:C2E1503 4.6 53.1 1.0 OE1 A:GLU1367 4.6 77.1 1.0 CB A:ASP1311 4.6 43.7 1.0 CZ A:ARG1334 4.6 87.2 1.0 OD2 A:ASP1310 4.7 42.0 1.0 O A:HOH1619 4.7 45.8 1.0 CD A:GLU1367 4.7 76.2 1.0 C4A A:C2E1503 4.8 58.5 1.0 NH2 A:ARG1334 4.8 91.6 1.0 C4' A:C2E1503 4.9 52.1 1.0 CG A:ASP1310 4.9 42.6 1.0

C.W.Phippen, H.Mikolajek, H.G.Schlaefli, C.W.Keevil, J.S.Webb, I.Tews. Phosphodiesterase Active Site Formation and Dimerisation of Pseudomonas Aeruginosa Mora, A Bi-Functional C-Di-Gmp Regulator Febs Lett..
ISSN: ISSN 0014-5793