Magnesium in PDB 4rum: Crystal Structure of the Nico Transition-Metal Riboswitch Bound to Cobalt

The structure of Crystal Structure of the Nico Transition-Metal Riboswitch Bound to Cobalt, PDB code: 4rum was solved by A.Ramesh, W.C.Winkler, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.87 / 2.64
Space group	I 4 2 2
Cell size a, b, c (Å), α, β, γ (°)	95.790, 95.790, 229.986, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 23.1

The structure of Crystal Structure of the Nico Transition-Metal Riboswitch Bound to Cobalt also contains other interesting chemical elements:

Cobalt	(Co)	4 atoms
Strontium	(Sr)	1 atom
Potassium	(K)	7 atoms

The binding sites of Magnesium atom in the Crystal Structure of the Nico Transition-Metal Riboswitch Bound to Cobalt (pdb code 4rum). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Nico Transition-Metal Riboswitch Bound to Cobalt, PDB code: 4rum:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Nico Transition-Metal Riboswitch Bound to Cobalt


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Nico Transition-Metal Riboswitch Bound to Cobalt within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg112 b:33.7 occ:1.00 O A:HOH228 2.3 39.8 1.0 OP2 A:A41 2.5 33.6 1.0 O6 A:G15 2.5 40.1 1.0 O A:HOH229 2.7 34.9 1.0 C6 A:G15 3.7 38.4 1.0 P A:A41 3.8 46.9 1.0 OP1 A:A41 4.4 50.5 1.0 O A:HOH247 4.6 45.7 1.0 O5' A:A41 4.6 43.7 1.0 C5 A:G15 4.6 40.3 1.0 N1 A:G15 4.7 34.2 1.0 N7 A:G15 4.7 36.7 1.0 C5' A:C40 4.9 30.6 1.0 O3' A:C40 4.9 36.3 1.0 C4' A:C40 4.9 30.0 1.0 OP2 A:C40 5.0 36.5 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Nico Transition-Metal Riboswitch Bound to Cobalt


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Nico Transition-Metal Riboswitch Bound to Cobalt within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg113 b:33.7 occ:1.00 O A:HOH286 2.5 41.6 1.0 O A:HOH285 2.7 46.8 1.0 O A:HOH284 3.2 41.2 1.0 OP2 A:A55 4.3 25.4 1.0 O A:HOH254 4.7 33.9 1.0 N1 A:G52 4.9 18.4 1.0

K.Furukawa, A.Ramesh, Z.Zhou, Z.Weinberg, T.Vallery, W.C.Winkler, R.R.Breaker. Bacterial Riboswitches Cooperatively Bind Ni(2+) or Co(2+) Ions and Control Expression of Heavy Metal Transporters. Mol.Cell V. 57 1088 2015.
ISSN: ISSN 1097-2765
PubMed: 25794617
DOI: 10.1016/J.MOLCEL.2015.02.009