Magnesium in PDB 4u3j: TOG2:Alpha/Beta-Tubulin Complex

The structure of TOG2:Alpha/Beta-Tubulin Complex, PDB code: 4u3j was solved by P.Ayaz, L.M.Rice, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.07 / 2.81
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	111.909, 89.573, 135.508, 90.00, 112.31, 90.00
R / Rfree (%)	21.8 / 25.9

The binding sites of Magnesium atom in the TOG2:Alpha/Beta-Tubulin Complex (pdb code 4u3j). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the TOG2:Alpha/Beta-Tubulin Complex, PDB code: 4u3j:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the TOG2:Alpha/Beta-Tubulin Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of TOG2:Alpha/Beta-Tubulin Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:40.0 occ:1.00 O A:HOH606 2.0 26.9 1.0 O A:HOH604 2.3 35.7 1.0 O A:HOH607 2.3 28.1 1.0 O2B A:GTP502 2.3 29.5 1.0 HB2 A:GLN11 2.9 38.7 1.0 O3G A:GTP502 3.0 25.0 1.0 O2G A:GTP502 3.1 40.5 1.0 PG A:GTP502 3.3 30.1 1.0 OE2 A:GLU72 3.4 37.9 1.0 H A:GLN11 3.5 36.0 1.0 PB A:GTP502 3.5 25.0 1.0 HZ1 B:LYS252 3.5 50.5 1.0 O3B A:GTP502 3.6 56.4 1.0 HB3 A:GLN11 3.7 38.7 1.0 CB A:GLN11 3.7 32.2 1.0 CD A:GLU72 3.7 38.5 1.0 O2A A:GTP502 3.9 34.0 1.0 OD2 A:ASP70 4.0 32.7 1.0 OE1 A:GLU72 4.0 39.8 1.0 OD1 A:ASP70 4.0 31.7 1.0 HG2 A:GLU72 4.0 45.2 1.0 N A:GLN11 4.1 30.0 1.0 OE1 A:GLN11 4.1 36.5 1.0 NZ B:LYS252 4.4 42.1 1.0 O3A A:GTP502 4.4 25.0 1.0 CA A:GLN11 4.4 30.7 1.0 CG A:ASP70 4.5 31.9 1.0 CG A:GLU72 4.5 37.7 1.0 HB2 A:ASP99 4.5 35.5 1.0 HZ3 B:LYS252 4.5 50.5 1.0 HG21 A:VAL75 4.6 41.7 1.0 O1B A:GTP502 4.6 33.7 1.0 HZ2 B:LYS252 4.6 50.5 1.0 HA A:GLN11 4.7 36.8 1.0 HA2 A:GLY10 4.7 34.6 1.0 HG23 A:VAL75 4.7 41.7 1.0 CD A:GLN11 4.7 35.4 1.0 PA A:GTP502 4.7 31.5 1.0 HB3 A:ASP99 4.7 35.5 1.0 CG A:GLN11 4.8 33.4 1.0 O1G A:GTP502 4.8 25.0 1.0 HB A:THR146 4.8 30.7 1.0 HB3 A:GLU72 4.9 46.4 1.0 HG1 A:THR146 5.0 31.1 1.0

Magnesium binding site 2 out of 2 in the TOG2:Alpha/Beta-Tubulin Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of TOG2:Alpha/Beta-Tubulin Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg501 b:40.0 occ:1.00 O3G B:GTP502 2.0 0.2 1.0 O B:HOH608 2.2 44.2 1.0 O2G B:GTP502 2.5 0.5 1.0 PG B:GTP502 2.7 0.4 1.0 HB2 B:GLN11 3.2 54.1 1.0 O3A B:GTP502 3.4 74.8 1.0 HB3 B:GLN11 3.7 54.1 1.0 O3B B:GTP502 3.8 77.9 1.0 H B:GLN11 3.9 49.4 1.0 OE1 B:GLN11 3.9 50.0 1.0 CB B:GLN11 3.9 45.1 1.0 O1G B:GTP502 3.9 0.8 1.0 O2B B:GTP502 3.9 81.1 1.0 OE1 B:GLU69 4.0 57.7 1.0 PB B:GTP502 4.0 77.5 1.0 HB3 B:GLU69 4.5 66.7 1.0 HG1 B:THR72 4.5 60.2 1.0 CD B:GLN11 4.6 49.9 1.0 N B:GLN11 4.6 41.2 1.0 PA B:GTP502 4.8 61.1 1.0 O2A B:GTP502 4.8 62.9 1.0 CG B:GLN11 4.8 46.9 1.0 HG1 B:THR143 4.9 53.9 1.0 CA B:GLN11 4.9 42.7 1.0 OD2 B:ASP67 4.9 45.8 1.0 OD1 B:ASP67 4.9 45.4 1.0 HG B:SER96 5.0 67.5 1.0 OG1 B:THR72 5.0 50.2 1.0

P.Ayaz, S.Munyoki, E.A.Geyer, F.A.Piedra, E.S.Vu, R.Bromberg, Z.Otwinowski, N.V.Grishin, C.A.Brautigam, L.M.Rice. A Tethered Delivery Mechanism Explains the Catalytic Action of A Microtubule Polymerase. Elife V. 3 03069 2014.
ISSN: ESSN 2050-084X
PubMed: 25097237
DOI: 10.7554/ELIFE.03069