Magnesium in PDB 4uhm: Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac

All present enzymatic activity of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac:
2.6.1.18;

The structure of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac, PDB code: 4uhm was solved by M.Wilding, T.S.Peat, J.Newman, C.Scott, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	89.47 / 1.33
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	62.877, 119.971, 134.310, 90.00, 90.00, 90.00
R / Rfree (%)	10.7 / 12.644

The structure of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Magnesium atom in the Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac (pdb code 4uhm). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac, PDB code: 4uhm:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1449 b:6.2 occ:0.50 O A:HOH2290 2.1 7.0 1.0 OD1 A:ASP180 2.1 5.7 1.0 O A:HOH2291 2.1 6.5 1.0 CG A:ASP180 3.1 6.5 1.0 OD2 A:ASP180 3.4 8.4 1.0 O A:HOH2293 4.1 12.9 1.0 O A:HIS181 4.2 7.0 1.0 N A:HIS181 4.3 5.2 1.0 O A:HOH2093 4.3 28.2 1.0 O A:HOH2292 4.5 10.8 1.0 CB A:ASP180 4.5 6.2 1.0 CD2 A:LEU211 4.6 13.2 1.0 CA A:ASP180 4.9 6.0 1.0

Magnesium binding site 2 out of 2 in the Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Characterization of A Novel Transaminase From Pseudomonas Sp. Strain Aac within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1450 b:16.4 occ:0.60 O A:HOH2164 1.9 28.6 1.0 O A:HOH2165 1.9 21.6 1.0 OD1 A:ASP112 2.2 13.1 1.0 O A:HOH2163 2.2 3.5 1.0 O A:ALA104 2.3 11.2 1.0 O A:HOH2179 2.6 24.4 1.0 C A:ALA104 3.2 10.0 1.0 CG A:ASP112 3.3 10.7 1.0 OD2 A:ASP112 3.7 13.6 1.0 N A:GLN105 4.0 9.9 0.5 N A:GLN105 4.0 10.5 0.5 O A:GLY109 4.1 11.0 1.0 CB A:ALA104 4.1 10.4 1.0 CA A:GLN105 4.1 15.1 0.5 CA A:ALA104 4.1 9.3 1.0 CA A:GLN105 4.1 10.2 0.5 O A:HOH2172 4.2 39.4 1.0 OG1 A:THR107 4.4 11.3 1.0 O A:HOH2178 4.4 32.6 1.0 O A:THR107 4.4 11.4 1.0 CB A:ASP112 4.5 8.2 1.0 O A:HOH2160 4.5 23.8 1.0 O A:LEU111 4.6 8.1 1.0 C A:GLN105 4.7 10.6 0.5 C A:GLN105 4.8 12.2 0.5 CA A:ASP112 4.8 7.5 1.0 CD A:GLN105 5.0 16.5 0.5 OE1 A:GLN105 5.0 23.7 0.5

M.Wilding, T.S.Peat, J.Newman, C.Scott. A Beta-Alanine Catabolism Pathway Containing A Highly Promiscuous Omega-Transaminase in the 12-Aminododecanate- Degrading Pseudomonas Sp. Strain Aac. Appl.Environ.Microbiol. V. 82 3846 2016.
ISSN: ISSN 0099-2240
PubMed: 27107110
DOI: 10.1128/AEM.00665-16