Magnesium in PDB 4usi: Nitrogen Regulatory Protein Pii From Chlamydomonas Reinhardtii in Complex with Mgatp and 2-Oxoglutarate

The structure of Nitrogen Regulatory Protein Pii From Chlamydomonas Reinhardtii in Complex with Mgatp and 2-Oxoglutarate, PDB code: 4usi was solved by V.R.Chellamuthu, K.Forchhammer, M.D.Hartmann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.90 / 1.45
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	42.040, 90.170, 46.180, 90.00, 96.47, 90.00
R / Rfree (%)	18.546 / 21.587

The binding sites of Magnesium atom in the Nitrogen Regulatory Protein Pii From Chlamydomonas Reinhardtii in Complex with Mgatp and 2-Oxoglutarate (pdb code 4usi). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Nitrogen Regulatory Protein Pii From Chlamydomonas Reinhardtii in Complex with Mgatp and 2-Oxoglutarate, PDB code: 4usi:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Nitrogen Regulatory Protein Pii From Chlamydomonas Reinhardtii in Complex with Mgatp and 2-Oxoglutarate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Nitrogen Regulatory Protein Pii From Chlamydomonas Reinhardtii in Complex with Mgatp and 2-Oxoglutarate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1151 b:16.0 occ:1.00 O2B A:ATP1152 2.0 15.6 1.0 O2G A:ATP1152 2.0 15.9 1.0 O1 A:AKG1153 2.1 16.4 1.0 O5 A:AKG1153 2.1 16.6 1.0 OE1 A:GLN56 2.1 18.3 1.0 O1A A:ATP1152 2.2 14.6 1.0 C1 A:AKG1153 2.8 16.9 1.0 C2 A:AKG1153 2.8 17.1 1.0 PB A:ATP1152 3.1 14.8 1.0 PG A:ATP1152 3.2 15.9 1.0 PA A:ATP1152 3.2 13.7 1.0 CD A:GLN56 3.3 18.8 1.0 O3B A:ATP1152 3.4 15.0 1.0 O3A A:ATP1152 3.5 13.8 1.0 O1G A:ATP1152 3.8 16.9 1.0 NE2 A:GLN56 3.9 18.9 1.0 N A:GLY105 3.9 15.8 1.0 N A:VAL55 4.0 16.6 1.0 O2 A:AKG1153 4.1 18.1 1.0 O A:HOH2067 4.1 15.7 1.0 CA A:GLY54 4.1 15.2 1.0 O2A A:ATP1152 4.2 13.4 1.0 CA A:GLY105 4.2 15.2 1.0 N A:GLN56 4.3 18.9 1.0 C3 A:AKG1153 4.3 19.6 1.0 C A:GLY54 4.4 16.3 1.0 O3G A:ATP1152 4.4 18.0 1.0 O5' A:ATP1152 4.4 13.1 1.0 CG A:GLN56 4.5 20.1 1.0 N A:GLY54 4.5 15.3 1.0 O1B A:ATP1152 4.5 14.7 1.0 CB A:GLN56 4.5 20.0 1.0 CB A:ILE104 4.5 24.4 1.0 CG2 A:VAL55 4.6 22.6 1.0 C A:ILE104 4.7 16.7 1.0 CA A:VAL55 4.9 20.0 1.0 C4 A:AKG1153 5.0 20.2 1.0 CA A:ILE104 5.0 17.4 1.0

Magnesium binding site 2 out of 2 in the Nitrogen Regulatory Protein Pii From Chlamydomonas Reinhardtii in Complex with Mgatp and 2-Oxoglutarate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Nitrogen Regulatory Protein Pii From Chlamydomonas Reinhardtii in Complex with Mgatp and 2-Oxoglutarate within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1151 b:24.6 occ:1.00 O1 B:AKG1153 1.9 21.2 0.5 O1A B:ATP1152 2.0 18.8 1.0 O2G B:ATP1152 2.0 21.8 1.0 O5 B:AKG1153 2.1 20.7 0.5 O2B B:ATP1152 2.1 20.9 1.0 OE1 B:GLN56 2.2 26.9 1.0 C1 B:AKG1153 2.7 21.3 0.5 C2 B:AKG1153 2.8 21.0 0.5 PB B:ATP1152 3.1 21.8 1.0 PA B:ATP1152 3.2 18.7 1.0 PG B:ATP1152 3.3 20.9 1.0 CD B:GLN56 3.3 39.7 1.0 O3B B:ATP1152 3.5 21.3 1.0 O3A B:ATP1152 3.5 19.6 1.0 N B:GLY105 3.8 19.4 1.0 O3G B:ATP1152 3.9 25.3 1.0 O2 B:AKG1153 3.9 21.7 0.5 NE2 B:GLN56 4.0 30.3 1.0 N B:VAL55 4.0 27.2 1.0 CA B:GLY54 4.0 22.0 1.0 CA B:GLY105 4.0 18.8 1.0 O B:HOH2069 4.1 24.9 1.0 O2A B:ATP1152 4.1 19.9 1.0 N B:GLN56 4.2 32.9 1.0 C3 B:AKG1153 4.3 21.3 0.5 C B:GLY54 4.3 23.3 1.0 O5' B:ATP1152 4.4 16.6 1.0 O1G B:ATP1152 4.4 20.0 1.0 N B:GLY54 4.5 21.6 1.0 CG B:GLN56 4.5 37.0 1.0 O1B B:ATP1152 4.6 23.8 1.0 CB B:GLN56 4.6 32.5 1.0 CG2 B:VAL55 4.6 38.1 1.0 C B:ILE104 4.7 20.2 1.0 C4 B:AKG1153 4.8 20.8 0.5 CB B:ILE104 4.9 28.8 1.0 CA B:VAL55 5.0 29.0 1.0

V.R.Chellamuthu, E.Ermilova, T.Lapina, J.Luddecke, E.Minaeva, C.Herrmann, M.D.Hartmann, K.Forchhammer. A Widespread Glutamine-Sensing Mechanism in the Plant Kingdom. Cell(Cambridge,Mass.) V. 159 1188 2014.
ISSN: ISSN 0092-8674
PubMed: 25416954
DOI: 10.1016/J.CELL.2014.10.015