Magnesium in PDB 4w5r: The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-8 (Long Target)

The structure of The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-8 (Long Target), PDB code: 4w5r was solved by N.T.Schirle, I.J.Macrae, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	69.63 / 2.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.378, 116.184, 69.678, 90.00, 92.17, 90.00
R / Rfree (%)	19.5 / 23.4

The binding sites of Magnesium atom in the The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-8 (Long Target) (pdb code 4w5r). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-8 (Long Target), PDB code: 4w5r:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-8 (Long Target)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-8 (Long Target) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg906 b:41.3 occ:1.00 O A:HOH1024 2.1 39.6 1.0 O A:HOH1023 2.1 37.7 1.0 O A:HOH1022 2.1 33.4 1.0 O A:HOH1025 2.1 45.3 1.0 OD1 A:ASP597 2.1 43.5 1.0 O A:VAL598 2.4 46.1 1.0 CG A:ASP597 3.0 44.6 1.0 C A:VAL598 3.1 40.8 1.0 OD2 A:ASP597 3.2 43.4 1.0 N A:VAL598 3.6 40.5 1.0 N A:THR599 3.8 41.4 1.0 CA A:VAL598 3.9 36.5 1.0 CA A:THR599 4.0 40.7 1.0 CE1 A:HIS807 4.3 46.9 1.0 CB A:ASP597 4.3 43.1 1.0 C A:ASP597 4.4 42.2 1.0 OD2 A:ASP669 4.5 44.7 1.0 O A:ASP669 4.6 36.0 1.0 CA A:ASP597 4.6 41.2 1.0 CB A:THR599 4.7 44.1 1.0 CB A:VAL598 4.8 36.0 1.0 CA A:GLY670 4.9 44.1 1.0 OE2 A:GLU637 4.9 45.5 1.0 ND1 A:HIS807 5.0 45.2 1.0

Magnesium binding site 2 out of 2 in the The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-8 (Long Target)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-8 (Long Target) within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg101 b:47.9 occ:1.00 O D:HOH202 2.1 43.4 1.0 O D:HOH201 2.1 51.5 1.0 O D:HOH204 2.1 52.6 1.0 O4 D:U6 2.3 42.2 1.0 C4 D:U6 3.4 47.6 1.0 C5 D:U6 3.9 42.6 1.0 O6 D:G7 4.4 40.9 1.0 N6 B:A6 4.4 37.5 1.0 N6 D:A5 4.4 47.6 1.0 N7 D:A5 4.5 57.6 1.0 N3 D:U6 4.6 42.8 1.0 N4 B:C5 5.0 36.9 1.0

N.T.Schirle, J.Sheu-Gruttadauria, I.J.Macrae. Gene Regulation. Structural Basis For Microrna Targeting. Science V. 346 608 2014.
ISSN: ESSN 1095-9203
PubMed: 25359968
DOI: 10.1126/SCIENCE.1258040