Magnesium in PDB 4w5t: The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-7

The structure of The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-7, PDB code: 4w5t was solved by N.T.Schirle, I.J.Macrae, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.29 / 2.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.636, 116.468, 69.822, 90.00, 92.33, 90.00
R / Rfree (%)	17.3 / 21.5

The binding sites of Magnesium atom in the The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-7 (pdb code 4w5t). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-7, PDB code: 4w5t:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-7


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-7 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg901 b:43.2 occ:1.00 O A:VAL598 2.0 40.5 1.0 O A:HOH1032 2.1 35.5 1.0 O A:HOH1033 2.1 27.2 1.0 O A:HOH1124 2.1 42.7 1.0 OD1 A:ASP597 2.1 46.1 1.0 O A:HOH1034 2.1 30.7 1.0 C A:VAL598 3.1 33.8 1.0 CG A:ASP597 3.2 47.6 1.0 O A:HOH1123 3.5 43.1 1.0 N A:VAL598 3.5 26.2 1.0 OD2 A:ASP597 3.6 47.8 1.0 CA A:VAL598 3.9 27.6 1.0 OD2 A:ASP669 4.0 54.9 1.0 O A:ASP669 4.1 36.2 1.0 N A:THR599 4.1 34.8 1.0 CA A:THR599 4.4 36.5 1.0 CB A:ASP597 4.4 45.1 1.0 OE2 A:GLU637 4.4 47.5 1.0 C A:ASP597 4.5 31.8 1.0 O B:HOH104 4.5 32.1 1.0 CB A:VAL598 4.6 25.0 1.0 CE1 A:HIS807 4.6 52.1 1.0 CA A:ASP597 4.6 37.6 1.0 CA A:GLY670 4.7 39.5 1.0 OE1 A:GLU637 4.7 42.1 1.0 C A:ASP669 4.8 36.0 1.0 OG1 A:THR599 4.9 42.5 1.0 O A:GLY670 4.9 53.9 1.0 CG A:ASP669 5.0 49.8 1.0

Magnesium binding site 2 out of 2 in the The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-7


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of The Crystal Structure of Human ARGONAUTE2 Bound to A Guide and Target Rna Containing Seed Pairing From 2-7 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg101 b:58.1 occ:1.00 O D:HOH201 2.1 38.6 1.0 O D:HOH202 2.1 35.4 1.0 O D:HOH204 2.1 42.2 1.0 O4 D:U4 2.5 35.8 1.0 C4 D:U4 3.4 37.9 1.0 C5 D:U4 3.7 36.0 1.0 O D:HOH205 4.0 55.9 1.0 O B:HOH108 4.3 33.6 1.0 N7 D:A3 4.5 47.7 1.0 O6 D:G5 4.6 35.7 1.0 N3 D:U4 4.6 38.6 1.0 N6 D:A3 4.8 35.7 1.0 C6 D:U4 5.0 36.6 1.0

N.T.Schirle, J.Sheu-Gruttadauria, I.J.Macrae. Gene Regulation. Structural Basis For Microrna Targeting. Science V. 346 608 2014.
ISSN: ESSN 1095-9203
PubMed: 25359968
DOI: 10.1126/SCIENCE.1258040