Magnesium in PDB 4w7s: Crystal Structure of the Yeast Dead-Box Splicing Factor PRP28 at 2.54 Angstroms Resolution

All present enzymatic activity of Crystal Structure of the Yeast Dead-Box Splicing Factor PRP28 at 2.54 Angstroms Resolution:
3.6.4.13;

The structure of Crystal Structure of the Yeast Dead-Box Splicing Factor PRP28 at 2.54 Angstroms Resolution, PDB code: 4w7s was solved by A.Jacewicz, P.Smith, B.Schwer, S.Shuman, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	53.84 / 2.54
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	114.719, 114.719, 156.186, 90.00, 90.00, 120.00
R / Rfree (%)	16.6 / 21.9

The binding sites of Magnesium atom in the Crystal Structure of the Yeast Dead-Box Splicing Factor PRP28 at 2.54 Angstroms Resolution (pdb code 4w7s). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Yeast Dead-Box Splicing Factor PRP28 at 2.54 Angstroms Resolution, PDB code: 4w7s:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Yeast Dead-Box Splicing Factor PRP28 at 2.54 Angstroms Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Yeast Dead-Box Splicing Factor PRP28 at 2.54 Angstroms Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg605 b:37.8 occ:1.00 O B:HOH701 2.0 35.2 1.0 O1B B:ANP601 2.3 34.2 1.0 O1A B:ANP601 2.3 28.7 1.0 O B:HOH800 2.3 31.7 1.0 O B:HOH704 2.7 37.5 1.0 PB B:ANP601 3.6 30.1 0.6 PA B:ANP601 3.6 27.7 0.8 O2G B:ANP601 3.7 39.4 1.0 O3A B:ANP601 3.9 36.1 1.0 O B:HOH801 3.9 33.1 0.9 O B:HOH717 4.0 31.9 1.0 O1G B:ANP601 4.1 43.6 1.0 PG B:ANP601 4.3 34.0 0.5 C5' B:ANP601 4.4 35.1 1.0 O5' B:ANP601 4.5 31.4 1.0 N3B B:ANP601 4.6 30.2 1.0 O2B B:ANP601 4.6 31.3 1.0 O2A B:ANP601 4.7 30.1 1.0 OE1 B:GLN269 4.7 53.4 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Yeast Dead-Box Splicing Factor PRP28 at 2.54 Angstroms Resolution


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Yeast Dead-Box Splicing Factor PRP28 at 2.54 Angstroms Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg606 b:38.7 occ:1.00 O A:HOH829 1.9 39.0 1.0 O A:HOH837 2.0 47.9 1.0 O A:HOH830 2.0 33.5 1.0 O B:HOH795 2.0 33.1 0.8 O B:HOH803 2.5 30.3 0.7 OE1 B:GLU352 4.2 38.1 1.0 OD1 B:ASP353 4.3 33.7 1.0 OD2 B:ASP353 4.3 37.2 1.0 OD1 A:ASP353 4.5 45.8 1.0 O B:HOH770 4.6 33.7 1.0 O A:HOH834 4.6 34.0 1.0 CG B:ASP353 4.7 28.8 1.0 O B:HOH779 4.8 31.5 1.0 OE1 A:GLU352 4.9 36.3 1.0 OD2 A:ASP353 4.9 45.1 1.0

A.Jacewicz, B.Schwer, P.Smith, S.Shuman. Crystal Structure, Mutational Analysis and Rna-Dependent Atpase Activity of the Yeast Dead-Box Pre-Mrna Splicing Factor PRP28. Nucleic Acids Res. 2014.
ISSN: ESSN 1362-4962
PubMed: 25303995
DOI: 10.1093/NAR/GKU930