Magnesium in PDB 4x9e: Deoxyguanosinetriphosphate Triphosphohydrolase From Escherichia Coli with Two Dna Effector Molecules

All present enzymatic activity of Deoxyguanosinetriphosphate Triphosphohydrolase From Escherichia Coli with Two Dna Effector Molecules:
3.1.5.1;

The structure of Deoxyguanosinetriphosphate Triphosphohydrolase From Escherichia Coli with Two Dna Effector Molecules, PDB code: 4x9e was solved by D.Singh, D.Gawel, M.Itsko, J.M.Krahn, R.E.London, R.M.Schaaper, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.63 / 3.10
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	136.800, 159.800, 190.000, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 22.3

The binding sites of Magnesium atom in the Deoxyguanosinetriphosphate Triphosphohydrolase From Escherichia Coli with Two Dna Effector Molecules (pdb code 4x9e). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Deoxyguanosinetriphosphate Triphosphohydrolase From Escherichia Coli with Two Dna Effector Molecules, PDB code: 4x9e:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Deoxyguanosinetriphosphate Triphosphohydrolase From Escherichia Coli with Two Dna Effector Molecules


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Deoxyguanosinetriphosphate Triphosphohydrolase From Escherichia Coli with Two Dna Effector Molecules within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1000 b:0.7 occ:1.00 OD2 A:ASP268 2.2 1.0 1.0 NE2 A:HIS117 2.3 0.1 1.0 NE2 A:HIS69 2.9 0.6 1.0 CD2 A:HIS117 3.2 0.4 1.0 CG A:ASP268 3.2 0.1 1.0 CE1 A:HIS117 3.4 0.6 1.0 OD1 A:ASP268 3.5 0.8 1.0 CD2 A:HIS69 3.7 0.9 1.0 OD2 A:ASP118 3.8 0.0 1.0 CE1 A:HIS69 3.9 0.7 1.0 OD1 A:ASP118 4.1 0.1 1.0 CG A:ASP118 4.3 0.8 1.0 CG A:HIS117 4.4 0.9 1.0 ND1 A:HIS117 4.5 0.9 1.0 CB A:ASP268 4.6 0.7 1.0 CG A:HIS69 4.9 0.9 1.0 NE2 A:GLN53 4.9 0.8 1.0 ND1 A:HIS69 4.9 0.7 1.0 NH1 A:ARG66 5.0 0.5 1.0

Magnesium binding site 2 out of 2 in the Deoxyguanosinetriphosphate Triphosphohydrolase From Escherichia Coli with Two Dna Effector Molecules


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Deoxyguanosinetriphosphate Triphosphohydrolase From Escherichia Coli with Two Dna Effector Molecules within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1000 b:0.2 occ:1.00 NE2 B:HIS117 2.3 0.6 1.0 OD2 B:ASP268 2.3 0.9 1.0 NE2 B:HIS69 3.0 0.6 1.0 CD2 B:HIS117 3.1 0.1 1.0 CG B:ASP268 3.3 0.7 1.0 CE1 B:HIS117 3.4 0.8 1.0 OD1 B:ASP268 3.6 0.8 1.0 OD2 B:ASP118 3.7 0.9 1.0 CD2 B:HIS69 3.7 0.5 1.0 CE1 B:HIS69 4.0 0.1 1.0 OD1 B:ASP118 4.0 0.3 1.0 CG B:ASP118 4.2 0.8 1.0 CG B:HIS117 4.3 0.8 1.0 ND1 B:HIS117 4.4 0.4 1.0 CB B:ASP268 4.6 0.6 1.0 CG B:HIS69 4.9 97.9 1.0 NE2 B:GLN53 4.9 0.2 1.0 ND1 B:HIS69 5.0 1.0 1.0

D.Singh, D.Gawel, M.Itsko, A.Hochkoeppler, J.M.Krahn, R.E.London, R.M.Schaaper. Structure of Escherichia Coli Dgtp Triphosphohydrolase: A Hexameric Enzyme with Dna Effector Molecules. J.Biol.Chem. 2015.
ISSN: ESSN 1083-351X
PubMed: 25694425
DOI: 10.1074/JBC.M115.636936