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Magnesium in PDB 4xiu: Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant with Dna Containing Ttt Overhang

Enzymatic activity of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant with Dna Containing Ttt Overhang

All present enzymatic activity of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant with Dna Containing Ttt Overhang:
2.7.7.7;

Protein crystallography data

The structure of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant with Dna Containing Ttt Overhang, PDB code: 4xiu was solved by E.Y.Wu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 47.64 / 2.50
Space group P 31 2 1
Cell size a, b, c (Å), α, β, γ (°) 110.030, 110.030, 90.670, 90.00, 90.00, 120.00
R / Rfree (%) 22.3 / 28.6

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant with Dna Containing Ttt Overhang (pdb code 4xiu). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant with Dna Containing Ttt Overhang, PDB code: 4xiu:

Magnesium binding site 1 out of 1 in 4xiu

Go back to Magnesium Binding Sites List in 4xiu
Magnesium binding site 1 out of 1 in the Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant with Dna Containing Ttt Overhang


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant with Dna Containing Ttt Overhang within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg901

b:45.0
occ:1.00
OD2 A:ASP610 2.4 52.8 1.0
OE1 A:GLU786 2.9 52.8 1.0
CG A:ASP610 3.5 44.3 1.0
CD A:GLU786 4.0 53.8 1.0
CB A:ASP610 4.2 40.7 1.0
OD1 A:ASP785 4.4 34.7 1.0
OD1 A:ASP610 4.5 45.0 1.0
OP1 B:DOC112 4.5 35.3 1.0
CG A:GLU786 4.7 47.4 1.0
OE2 A:GLU786 4.8 58.3 1.0
CB A:GLU786 4.9 42.6 1.0
O A:GLU832 4.9 91.1 1.0
C3' B:DOC112 5.0 34.3 1.0
C A:GLU832 5.0 87.9 1.0
C5' B:DOC112 5.0 35.3 1.0

Reference:

E.Y.Wu, A.R.Walsh, E.C.Materne, E.P.Hiltner, B.Zielinski, B.R.Miller, L.Mawby, E.Modeste, C.A.Parish, W.M.Barnes, M.B.Kermekchiev. A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KLENTAQ1 Dna Polymerase. Biochemistry V. 54 881 2015.
ISSN: ISSN 0006-2960
PubMed: 25537790
DOI: 10.1021/BI501198F
Page generated: Mon Dec 14 19:46:42 2020

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