Magnesium in PDB 4xln: Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna

All present enzymatic activity of Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna:
2.7.7.6;

The structure of Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna, PDB code: 4xln was solved by B.Bae, S.A.Darst, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	34.96 / 4.00
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	289.259, 289.259, 536.601, 90.00, 90.00, 90.00
R / Rfree (%)	28 / 31.5

The structure of Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Magnesium atom in the Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna (pdb code 4xln). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna, PDB code: 4xln:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg2003 b:0.8 occ:1.00 O3' Q:A4 1.9 0.6 1.0 OD1 D:ASP743 2.0 0.4 1.0 OD2 D:ASP743 2.1 94.0 1.0 OD1 D:ASP739 2.2 0.6 1.0 OD2 D:ASP741 2.2 0.8 1.0 CG D:ASP743 2.3 0.5 1.0 CG D:ASP741 2.9 0.3 1.0 OD1 D:ASP741 2.9 96.3 1.0 O2' Q:A4 3.0 0.1 1.0 C3' Q:A4 3.1 0.3 1.0 CG D:ASP739 3.1 0.1 1.0 OD2 D:ASP739 3.4 0.1 1.0 C2' Q:A4 3.5 0.7 1.0 C4' Q:A4 3.7 0.1 1.0 CB D:ASP743 3.8 90.2 1.0 N D:ASP739 4.2 86.5 1.0 O D:ASP739 4.4 0.6 1.0 CB D:ASP741 4.4 95.0 1.0 CB D:ASP739 4.4 0.1 1.0 N D:ASP741 4.5 0.3 1.0 C5' Q:A4 4.5 98.5 1.0 CA D:ASP743 4.6 79.1 1.0 C D:ASP739 4.6 93.4 1.0 CA D:ASP739 4.7 99.5 1.0 N D:ASP743 4.7 93.6 1.0 C1' Q:A4 4.8 0.6 1.0 O4' Q:A4 4.8 99.5 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of T. Aquaticus Transcription Initiation Complex Containing Bubble Promoter and Rna within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg2003 b:0.6 occ:1.00 OD1 J:ASP743 1.9 0.8 1.0 OD2 J:ASP743 2.0 0.9 1.0 OD1 J:ASP739 2.1 0.4 1.0 O3' T:A4 2.1 0.5 1.0 OD2 J:ASP741 2.2 0.1 1.0 CG J:ASP743 2.2 0.3 1.0 CG J:ASP741 2.9 0.0 1.0 CG J:ASP739 3.0 1.0 1.0 O2' T:A4 3.0 0.6 1.0 OD1 J:ASP741 3.0 0.5 1.0 C3' T:A4 3.2 0.8 1.0 OD2 J:ASP739 3.3 0.3 1.0 C4' T:A4 3.6 0.2 1.0 C2' T:A4 3.6 0.5 1.0 CB J:ASP743 3.7 1.0 1.0 N J:ASP739 4.0 1.0 1.0 O J:ASP739 4.2 0.4 1.0 CB J:ASP739 4.3 0.6 1.0 N J:ASP741 4.4 0.7 1.0 C J:ASP739 4.4 0.0 1.0 CB J:ASP741 4.4 0.7 1.0 C5' T:A4 4.5 0.4 1.0 CA J:ASP739 4.5 0.9 1.0 CA J:ASP743 4.5 96.3 1.0 N J:ASP743 4.6 86.8 1.0 O4' T:A4 4.7 0.9 1.0 C1' T:A4 4.8 88.2 1.0 CA J:ASP741 5.0 0.1 1.0

B.Bae, A.Feklistov, A.Lass-Napiorkowska, R.Landick, S.A.Darst. Structure of A Bacterial Rna Polymerase Holoenzyme Open Promoter Complex. Elife V. 4 2015.
ISSN: ESSN 2050-084X
PubMed: 26349032
DOI: 10.7554/ELIFE.08504