Magnesium in PDB 4xlv: Crystal Structure of the Activated Insulin Receptor Tyrosine Kinase Dimer

All present enzymatic activity of Crystal Structure of the Activated Insulin Receptor Tyrosine Kinase Dimer:
2.7.10.1;

The structure of Crystal Structure of the Activated Insulin Receptor Tyrosine Kinase Dimer, PDB code: 4xlv was solved by S.R.Hubbard, S.Li, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.30
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	66.982, 66.982, 136.580, 90.00, 90.00, 120.00
R / Rfree (%)	20.4 / 26.6

The binding sites of Magnesium atom in the Crystal Structure of the Activated Insulin Receptor Tyrosine Kinase Dimer (pdb code 4xlv). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Activated Insulin Receptor Tyrosine Kinase Dimer, PDB code: 4xlv:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Activated Insulin Receptor Tyrosine Kinase Dimer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Activated Insulin Receptor Tyrosine Kinase Dimer within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1302 b:23.3 occ:1.00 O1A A:ACP1301 2.0 32.6 1.0 OD2 A:ASP1150 2.1 23.6 1.0 O A:HOH1456 2.1 21.7 1.0 O1B A:ACP1301 2.2 32.7 1.0 OD1 A:ASN1137 2.3 24.4 1.0 PB A:ACP1301 3.2 37.0 1.0 CG A:ASP1150 3.2 23.2 1.0 PA A:ACP1301 3.2 36.4 1.0 CG A:ASN1137 3.4 24.0 1.0 O3A A:ACP1301 3.5 38.8 1.0 O1G A:ACP1301 3.7 42.9 1.0 C3B A:ACP1301 3.8 39.4 1.0 CB A:ASP1150 3.8 22.8 1.0 ND2 A:ASN1137 4.0 23.2 1.0 O A:ARG1136 4.2 25.7 1.0 OD1 A:ASP1150 4.2 24.1 1.0 O5' A:ACP1301 4.3 35.8 1.0 O2A A:ACP1301 4.3 34.1 1.0 PG A:ACP1301 4.5 51.9 1.0 C5' A:ACP1301 4.6 34.4 1.0 CB A:ASN1137 4.6 22.7 1.0 O2B A:ACP1301 4.6 38.4 1.0 C A:ARG1136 4.7 23.5 1.0 CG A:ARG1136 4.8 23.5 1.0 CA A:ASN1137 4.8 23.7 1.0 O A:HOH1487 4.8 48.7 1.0 N A:ASN1137 5.0 23.9 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Activated Insulin Receptor Tyrosine Kinase Dimer


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Activated Insulin Receptor Tyrosine Kinase Dimer within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1303 b:29.4 occ:1.00 O A:HOH1478 1.9 50.6 1.0 O2B A:ACP1301 2.1 38.4 1.0 O A:HOH1487 2.4 48.7 1.0 O3G A:ACP1301 2.8 46.6 1.0 PB A:ACP1301 3.1 37.0 1.0 O1B A:ACP1301 3.3 32.7 1.0 CA A:GLY1005 3.5 59.6 1.0 N A:SER1006 3.6 62.3 1.0 PG A:ACP1301 3.7 51.9 1.0 C3B A:ACP1301 3.9 39.4 1.0 O1G A:ACP1301 4.0 42.9 1.0 C A:GLY1005 4.1 61.4 1.0 OG A:SER1006 4.2 65.9 1.0 O3A A:ACP1301 4.5 38.8 1.0 CE2 A:PHE1007 4.6 49.9 1.0 N A:PHE1007 4.7 57.1 1.0 CD2 A:PHE1007 4.7 51.1 1.0 N A:GLY1005 4.8 55.9 1.0 CA A:SER1006 4.8 60.6 1.0 N A:GLY1008 4.8 61.9 1.0 CB A:SER1006 5.0 61.0 1.0

M.Z.Cabail, S.Li, E.Lemmon, M.E.Bowen, S.R.Hubbard, W.T.Miller. The Insulin and IGF1 Receptor Kinase Domains Are Functional Dimers in the Activated State. Nat Commun V. 6 6406 2015.
ISSN: ESSN 2041-1723
PubMed: 25758790
DOI: 10.1038/NCOMMS7406