Magnesium in PDB 4xvq: H-Ras Y137E

All present enzymatic activity of H-Ras Y137E:
3.6.5.2;

The structure of H-Ras Y137E, PDB code: 4xvq was solved by C.W.Johnson, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.49 / 1.89
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	39.046, 39.046, 158.737, 90.00, 90.00, 120.00
R / Rfree (%)	20.3 / 25.4

The binding sites of Magnesium atom in the H-Ras Y137E (pdb code 4xvq). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the H-Ras Y137E, PDB code: 4xvq:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the H-Ras Y137E


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of H-Ras Y137E within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:22.5 occ:1.00 O1G A:GNP202 1.9 25.1 1.0 O1B A:GNP202 2.0 21.6 1.0 O A:HOH316 2.0 23.2 1.0 OG1 A:THR35 2.1 27.2 1.0 O A:HOH352 2.1 20.9 1.0 OG A:SER17 2.2 20.2 1.0 CB A:SER17 3.1 19.4 1.0 PB A:GNP202 3.1 21.4 1.0 PG A:GNP202 3.1 27.4 1.0 CB A:THR35 3.2 28.0 1.0 N3B A:GNP202 3.3 23.2 1.0 N A:THR35 3.8 25.0 1.0 N A:SER17 3.8 19.1 1.0 CA A:SER17 4.0 21.8 1.0 OD2 A:ASP57 4.0 25.5 1.0 O2A A:GNP202 4.1 23.9 1.0 O3G A:GNP202 4.1 22.4 1.0 CA A:THR35 4.1 26.7 1.0 O3A A:GNP202 4.2 18.7 1.0 O2B A:GNP202 4.2 21.1 1.0 OD1 A:ASP57 4.2 21.8 1.0 O2G A:GNP202 4.3 25.8 1.0 CG2 A:THR35 4.3 25.5 1.0 O A:ASP33 4.4 27.6 1.0 O A:THR58 4.4 27.1 1.0 PA A:GNP202 4.4 25.1 1.0 O A:HOH372 4.5 34.4 1.0 CG A:ASP57 4.5 24.8 1.0 O1A A:GNP202 4.6 22.6 1.0 C A:PRO34 4.7 29.4 1.0 CB A:LYS16 4.9 19.5 1.0 C A:LYS16 4.9 21.9 1.0 CA A:PRO34 5.0 29.4 1.0

Magnesium binding site 2 out of 2 in the H-Ras Y137E


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of H-Ras Y137E within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg203 b:26.4 occ:1.00 O A:ASN26 2.6 38.3 1.0 O A:HOH318 2.6 42.0 1.0 C A:ASN26 3.7 34.1 1.0 O A:HOH365 4.2 39.7 1.0 CB A:ASN26 4.4 31.4 1.0 CA A:HIS27 4.4 32.1 1.0 N A:HIS27 4.5 30.9 1.0 CA A:ASN26 4.6 28.9 1.0 OE1 A:GLN22 4.7 33.7 1.0 CB A:HIS27 4.9 30.1 1.0

P.Y.Ting, C.W.Johnson, C.Fang, X.Cao, T.G.Graeber, C.Mattos, J.Colicelli. Tyrosine Phosphorylation of Ras By Abl Allosterically Enhances Effector Binding. Faseb J. V. 29 3750 2015.
ISSN: ESSN 1530-6860
PubMed: 25999467
DOI: 10.1096/FJ.15-271510