Magnesium in PDB 4yws: Thermostable Enolase From Chloroflexus Aurantiacus

All present enzymatic activity of Thermostable Enolase From Chloroflexus Aurantiacus:
4.2.1.11;

The structure of Thermostable Enolase From Chloroflexus Aurantiacus, PDB code: 4yws was solved by O.A.Zadvornyy, J.W.Peters, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.76 / 2.45
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	146.319, 146.319, 102.989, 90.00, 90.00, 90.00
R / Rfree (%)	19.9 / 24

The binding sites of Magnesium atom in the Thermostable Enolase From Chloroflexus Aurantiacus (pdb code 4yws). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Thermostable Enolase From Chloroflexus Aurantiacus, PDB code: 4yws:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Thermostable Enolase From Chloroflexus Aurantiacus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Thermostable Enolase From Chloroflexus Aurantiacus within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:51.7 occ:1.00 O A:HOH649 1.9 30.0 1.0 OE1 A:GLU286 2.2 53.8 1.0 O A:HOH641 2.4 30.0 1.0 O A:HOH623 2.5 57.0 1.0 OD2 A:ASP243 2.6 75.7 1.0 OD2 A:ASP313 2.6 78.3 1.0 CG A:ASP313 3.0 74.4 1.0 CG A:ASP243 3.2 69.5 1.0 OD1 A:ASP243 3.4 70.5 1.0 CD A:GLU286 3.4 69.0 1.0 CB A:ASP313 3.6 67.6 1.0 OD1 A:ASP313 3.6 73.5 1.0 NZ A:LYS389 3.7 60.4 1.0 CG A:GLU286 4.2 56.5 1.0 OE2 A:GLU286 4.2 57.4 1.0 CD1 A:LEU336 4.5 47.6 1.0 OD2 A:ASP287 4.5 79.2 1.0 CB A:ASP243 4.5 56.9 1.0 OE2 A:GLU165 4.6 91.5 1.0 CE A:LYS389 4.9 56.1 1.0

Magnesium binding site 2 out of 2 in the Thermostable Enolase From Chloroflexus Aurantiacus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Thermostable Enolase From Chloroflexus Aurantiacus within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg501 b:71.4 occ:1.00 OD2 B:ASP313 2.1 95.4 1.0 O B:HOH601 2.4 30.0 1.0 OE1 B:GLU286 2.5 72.8 1.0 OD2 B:ASP243 2.6 94.1 1.0 OD1 B:ASP243 3.1 84.7 1.0 CG B:ASP243 3.1 85.4 1.0 CG B:ASP313 3.2 91.2 1.0 CD B:GLU286 3.5 86.5 1.0 CB B:ASP313 3.8 82.5 1.0 CG B:GLU286 4.1 71.0 1.0 NZ B:LYS389 4.2 82.4 1.0 OD2 B:ASP287 4.2 96.6 1.0 OD1 B:ASP313 4.3 91.6 1.0 OE2 B:GLU286 4.4 76.4 1.0 CB B:ASP243 4.5 71.9 1.0 CG B:ASP287 4.8 90.8 1.0 OE2 B:GLU165 4.9 0.9 1.0 CE B:LYS389 4.9 71.8 1.0 CD2 B:LEU336 4.9 57.9 1.0

O.A.Zadvornyy, E.S.Boyd, M.C.Posewitz, N.A.Zorin, J.W.Peters. Biochemical and Structural Characterization of Enolase From Chloroflexus Aurantiacus: Evidence For A Thermophilic Origin. Front Bioeng Biotechnol V. 3 74 2015.
ISSN: ISSN 2296-4185
PubMed: 26082925
DOI: 10.3389/FBIOE.2015.00074