Magnesium in PDB 4z17: Thermostable Enolase From Chloroflexus Aurantiacus

All present enzymatic activity of Thermostable Enolase From Chloroflexus Aurantiacus:
4.2.1.11;

The structure of Thermostable Enolase From Chloroflexus Aurantiacus, PDB code: 4z17 was solved by O.A.Zadvornyy, J.W.Peters, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.68 / 2.65
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	146.279, 146.279, 101.777, 90.00, 90.00, 90.00
R / Rfree (%)	19.3 / 23.8

The binding sites of Magnesium atom in the Thermostable Enolase From Chloroflexus Aurantiacus (pdb code 4z17). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Thermostable Enolase From Chloroflexus Aurantiacus, PDB code: 4z17:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Thermostable Enolase From Chloroflexus Aurantiacus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Thermostable Enolase From Chloroflexus Aurantiacus within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:64.7 occ:1.00 OD2 A:ASP313 2.3 75.8 1.0 OD1 A:ASP243 2.7 68.3 1.0 OD2 A:ASP243 2.8 75.8 1.0 OE1 A:GLU286 3.1 66.3 1.0 CG A:ASP243 3.1 68.3 1.0 CG A:ASP313 3.4 72.3 1.0 O1 A:PEP502 3.4 0.2 1.0 MG A:MG503 3.5 78.8 1.0 OD1 A:ASP287 3.7 71.8 1.0 CB A:ASP313 3.8 63.1 1.0 O2' A:PEP502 3.9 0.2 1.0 OD2 A:ASP314 4.0 92.9 1.0 CD A:GLU286 4.0 69.9 1.0 C1 A:PEP502 4.1 0.3 1.0 CB A:ALA245 4.1 67.2 1.0 CG A:GLU286 4.4 55.0 1.0 CG A:ASP287 4.4 71.4 1.0 OD1 A:ASP313 4.5 72.1 1.0 CA A:ALA245 4.6 66.6 1.0 CB A:ASP243 4.6 58.9 1.0 CB A:ASP287 4.7 60.8 1.0 CG A:ASP314 4.8 85.8 1.0 NZ A:LYS389 4.8 60.2 1.0

Magnesium binding site 2 out of 4 in the Thermostable Enolase From Chloroflexus Aurantiacus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Thermostable Enolase From Chloroflexus Aurantiacus within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg503 b:78.8 occ:1.00 O1 A:PEP502 2.8 0.2 1.0 OD2 A:ASP313 3.5 75.8 1.0 OD2 A:ASP314 3.5 92.9 1.0 MG A:MG501 3.5 64.7 1.0 O1P A:PEP502 3.5 0.5 1.0 OD1 A:ASP314 3.6 87.6 1.0 C1 A:PEP502 3.7 0.3 1.0 CG A:ASP314 3.9 85.8 1.0 NZ A:LYS338 4.1 82.9 1.0 CG2 A:THR44 4.3 75.9 0.5 O2' A:PEP502 4.3 0.2 1.0 CG A:ASP313 4.4 72.3 1.0 P A:PEP502 4.5 0.5 1.0 CB A:ALA42 4.6 63.4 0.5 C2 A:PEP502 4.6 0.8 1.0 O3P A:PEP502 4.7 0.8 1.0 O2 A:PEP502 4.7 0.9 1.0 OD1 A:ASP313 4.7 72.1 1.0

Magnesium binding site 3 out of 4 in the Thermostable Enolase From Chloroflexus Aurantiacus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Thermostable Enolase From Chloroflexus Aurantiacus within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg501 b:75.1 occ:1.00 OD2 B:ASP313 2.5 98.2 1.0 O2' B:PEP502 2.6 0.5 1.0 OD2 B:ASP243 2.6 0.6 1.0 OE1 B:GLU286 3.1 76.1 1.0 OD1 B:ASP243 3.3 94.3 1.0 CG B:ASP243 3.3 94.7 1.0 MG B:MG503 3.5 73.6 1.0 CG B:ASP313 3.7 89.7 1.0 C1 B:PEP502 3.8 0.2 1.0 OD1 B:ASP287 3.9 92.4 1.0 CD B:GLU286 4.2 86.3 1.0 OE2 B:GLU165 4.2 0.7 1.0 CB B:ALA245 4.2 98.2 1.0 CB B:ASP313 4.3 76.3 1.0 NZ B:LYS389 4.3 83.6 1.0 C2 B:PEP502 4.6 0.1 1.0 C3 B:PEP502 4.6 0.7 1.0 O1 B:PEP502 4.7 0.8 1.0 OD1 B:ASP313 4.7 89.2 1.0 NE2 B:GLN164 4.7 0.7 1.0 CG B:ASP287 4.7 92.3 1.0 CB B:ASP243 4.8 83.7 1.0 CG B:GLU286 4.9 76.9 1.0 CD B:GLU165 4.9 0.8 1.0 OD2 B:ASP314 4.9 0.6 1.0 OE2 B:GLU286 5.0 68.7 1.0 OE1 B:GLU165 5.0 95.2 1.0

Magnesium binding site 4 out of 4 in the Thermostable Enolase From Chloroflexus Aurantiacus


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Thermostable Enolase From Chloroflexus Aurantiacus within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg503 b:73.6 occ:1.00 CB B:SER43 2.7 0.1 1.0 O2P B:PEP502 3.0 0.8 1.0 O2' B:PEP502 3.5 0.5 1.0 MG B:MG501 3.5 75.1 1.0 OG B:SER43 3.6 0.2 1.0 CA B:SER43 3.8 0.8 1.0 OD2 B:ASP313 3.8 98.2 1.0 C2 B:PEP502 3.9 0.1 1.0 OD2 B:ASP314 3.9 0.6 1.0 C3 B:PEP502 3.9 0.7 1.0 P B:PEP502 4.1 0.0 1.0 C1 B:PEP502 4.1 0.2 1.0 OD1 B:ASP314 4.1 94.1 1.0 O3P B:PEP502 4.2 0.2 1.0 NZ B:LYS338 4.2 80.0 1.0 CG B:ASP314 4.4 94.5 1.0 O2 B:PEP502 4.5 0.8 1.0 N B:SER43 4.6 1.0 1.0 C B:SER43 4.8 0.3 1.0 CG B:ASP313 4.8 89.7 1.0

O.A.Zadvornyy, E.S.Boyd, M.C.Posewitz, N.A.Zorin, J.W.Peters. Biochemical and Structural Characterization of Enolase From Chloroflexus Aurantiacus: Evidence For A Thermophilic Origin. Front Bioeng Biotechnol V. 3 74 2015.
ISSN: ISSN 2296-4185
PubMed: 26082925
DOI: 10.3389/FBIOE.2015.00074