Magnesium in PDB 4z1y: Thermostable Enolase From Chloroflexus Aurantiacus with Substrate 2- Phosphoglycerate

All present enzymatic activity of Thermostable Enolase From Chloroflexus Aurantiacus with Substrate 2- Phosphoglycerate:
4.2.1.11;

The structure of Thermostable Enolase From Chloroflexus Aurantiacus with Substrate 2- Phosphoglycerate, PDB code: 4z1y was solved by O.A.Zadvornyy, J.W.Peters, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.70 / 2.53
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	146.324, 146.324, 101.883, 90.00, 90.00, 90.00
R / Rfree (%)	21.4 / 25.2

The binding sites of Magnesium atom in the Thermostable Enolase From Chloroflexus Aurantiacus with Substrate 2- Phosphoglycerate (pdb code 4z1y). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Thermostable Enolase From Chloroflexus Aurantiacus with Substrate 2- Phosphoglycerate, PDB code: 4z1y:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Thermostable Enolase From Chloroflexus Aurantiacus with Substrate 2- Phosphoglycerate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Thermostable Enolase From Chloroflexus Aurantiacus with Substrate 2- Phosphoglycerate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:41.1 occ:1.00 OD2 A:ASP313 2.1 59.9 1.0 OE1 A:GLU286 2.3 54.5 1.0 OD2 A:ASP243 2.3 73.9 1.0 CG A:ASP243 3.1 68.6 1.0 CG A:ASP313 3.2 56.7 1.0 OD1 A:ASP243 3.3 70.6 1.0 O1 A:2PG502 3.4 93.8 0.7 CD A:GLU286 3.5 66.2 1.0 CB A:ASP313 3.7 52.6 1.0 NZ A:LYS389 3.8 54.4 1.0 O A:HOH611 4.1 30.0 1.0 OD1 A:ASP313 4.2 55.4 1.0 OE2 A:GLU286 4.3 61.2 1.0 OD1 A:ASP287 4.3 66.0 1.0 CG A:GLU286 4.4 51.2 1.0 CB A:ASP243 4.4 55.3 1.0 OE2 A:GLU165 4.5 78.0 1.0 CD2 A:LEU336 4.5 39.6 1.0 C1 A:2PG502 4.6 94.9 0.7 NZ A:LYS338 4.6 69.8 1.0

Magnesium binding site 2 out of 2 in the Thermostable Enolase From Chloroflexus Aurantiacus with Substrate 2- Phosphoglycerate


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Thermostable Enolase From Chloroflexus Aurantiacus with Substrate 2- Phosphoglycerate within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg501 b:56.3 occ:1.00 OD2 B:ASP313 2.2 81.1 1.0 OE1 B:GLU286 2.3 65.7 1.0 OD2 B:ASP243 2.4 88.1 1.0 O2 B:2PG502 2.5 99.4 0.7 CG B:ASP243 3.2 84.7 1.0 CG B:ASP313 3.3 75.2 1.0 OD1 B:ASP243 3.3 85.0 1.0 CD B:GLU286 3.5 73.2 1.0 C1 B:2PG502 3.7 99.7 0.7 CB B:ASP313 3.7 69.8 1.0 OD1 B:ASP287 4.2 78.1 1.0 O1 B:2PG502 4.2 99.1 0.7 CG B:GLU286 4.3 67.0 1.0 OE2 B:GLU286 4.4 60.0 1.0 OD1 B:ASP313 4.4 73.3 1.0 NZ B:LYS389 4.5 80.0 1.0 OE2 B:GLU165 4.6 0.5 1.0 CB B:ASP243 4.7 77.1 1.0 CD2 B:LEU336 4.7 50.4 1.0 NZ B:LYS338 4.8 86.1 1.0 OD2 B:ASP314 4.8 91.5 1.0 OE1 B:GLU165 4.9 0.1 1.0 C2 B:2PG502 4.9 0.0 0.7 O1P B:2PG502 4.9 0.8 0.7 CG B:ASP287 5.0 77.8 1.0

O.A.Zadvornyy, E.S.Boyd, M.C.Posewitz, N.A.Zorin, J.W.Peters. Biochemical and Structural Characterization of Enolase From Chloroflexus Aurantiacus: Evidence For A Thermophilic Origin. Front Bioeng Biotechnol V. 3 74 2015.
ISSN: ISSN 2296-4185
PubMed: 26082925
DOI: 10.3389/FBIOE.2015.00074