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Magnesium in PDB 4z6c: Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp

Enzymatic activity of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp

All present enzymatic activity of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp:
2.7.7.7;

Protein crystallography data

The structure of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp, PDB code: 4z6c was solved by M.-C.Koag, S.Lee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.68
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 48.180, 79.274, 54.372, 90.00, 105.96, 90.00
R / Rfree (%) 20.1 / 27.2

Other elements in 4z6c:

The structure of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp also contains other interesting chemical elements:

Sodium (Na) 2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp (pdb code 4z6c). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp, PDB code: 4z6c:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in 4z6c

Go back to Magnesium Binding Sites List in 4z6c
Magnesium binding site 1 out of 2 in the Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
P:Mg101

b:26.9
occ:1.00
OD1 A:ASP192 2.2 25.6 1.0
OD2 A:ASP256 2.3 30.5 1.0
O1A A:0KX402 2.4 22.7 1.0
O3' P:DA10 2.6 23.4 1.0
OD2 A:ASP190 2.7 31.9 1.0
OD1 A:ASP190 2.9 27.7 1.0
CG A:ASP190 3.1 25.6 1.0
CG A:ASP192 3.3 27.3 1.0
PA A:0KX402 3.4 24.3 1.0
CG A:ASP256 3.5 23.6 1.0
MG A:MG401 3.5 27.1 1.0
C3' P:DA10 3.7 24.6 1.0
O2A A:0KX402 3.8 25.2 1.0
OD2 A:ASP192 3.8 25.9 1.0
O5' A:0KX402 4.0 24.6 1.0
CB A:ASP256 4.1 21.5 1.0
C5' A:0KX402 4.2 25.1 1.0
C5' P:DA10 4.3 27.7 1.0
C4' P:DA10 4.3 27.8 1.0
OD1 A:ASP256 4.4 27.4 1.0
CB A:ASP190 4.5 24.5 1.0
O1G A:0KX402 4.6 26.3 1.0
OP1 P:DA10 4.6 34.5 1.0
CB A:ASP192 4.6 29.6 1.0
O5' P:DA10 4.6 31.4 1.0
O A:MET191 4.8 26.8 1.0
N3A A:0KX402 4.9 25.3 1.0
NH2 A:ARG254 4.9 22.9 1.0
C2' P:DA10 5.0 25.2 1.0

Magnesium binding site 2 out of 2 in 4z6c

Go back to Magnesium Binding Sites List in 4z6c
Magnesium binding site 2 out of 2 in the Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Human Dna Polymerase Beta 279NA Mutant Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ctp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg401

b:27.1
occ:1.00
OD2 A:ASP192 2.0 25.9 1.0
O1A A:0KX402 2.0 22.7 1.0
O A:HOH503 2.1 31.1 1.0
OD1 A:ASP190 2.2 27.7 1.0
O2B A:0KX402 2.2 24.7 1.0
O1G A:0KX402 2.3 26.3 1.0
CG A:ASP192 2.9 27.3 1.0
OD1 A:ASP192 3.1 25.6 1.0
PB A:0KX402 3.2 26.6 1.0
PA A:0KX402 3.3 24.3 1.0
CG A:ASP190 3.4 25.6 1.0
MG P:MG101 3.5 26.9 1.0
PG A:0KX402 3.5 23.3 1.0
O A:ASP190 3.6 28.4 1.0
N3A A:0KX402 3.6 25.3 1.0
O3B A:0KX402 3.7 24.5 1.0
O A:HOH502 3.9 32.0 1.0
O3G A:0KX402 4.0 22.0 1.0
C A:ASP190 4.0 27.7 1.0
OD2 A:ASP190 4.1 31.9 1.0
N A:ASP190 4.2 26.6 1.0
C5' A:0KX402 4.2 25.1 1.0
O5' A:0KX402 4.3 24.6 1.0
CA A:GLY179 4.3 28.1 1.0
CB A:ASP192 4.3 29.6 1.0
N A:SER180 4.4 36.4 1.0
CA A:ASP190 4.4 26.5 1.0
O2A A:0KX402 4.5 25.2 1.0
CB A:ASP190 4.5 24.5 1.0
OG A:SER180 4.6 26.1 1.0
N A:ASP192 4.6 24.6 1.0
O1B A:0KX402 4.7 27.3 1.0
N A:MET191 4.7 23.3 1.0
O2G A:0KX402 4.8 22.7 1.0
C A:MET191 4.9 26.1 1.0
C A:GLY179 4.9 35.9 1.0

Reference:

M.C.Koag, S.Lee. Insights Into the Effect of Minor Groove Interactions and Metal Cofactors on Mutagenic Replication By Human Dna Polymerase Beta. Biochem. J. V. 475 571 2018.
ISSN: ESSN 1470-8728
PubMed: 29301983
DOI: 10.1042/BCJ20170787
Page generated: Sat Sep 28 23:57:01 2024

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