Magnesium in PDB 4zfh: Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A

The structure of Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A, PDB code: 4zfh was solved by P.Chuankhayan, S.Saoin, K.Chupradit, T.Wisitponchai, K.Intachai, K.Kitidee, S.Nangola, L.V.Sanghiran, S.S.Hong, P.Boulanger, C.Tayapiwatana, C.J.Chen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 1.89
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	104.839, 28.638, 53.537, 90.00, 106.39, 90.00
R / Rfree (%)	21 / 27.5

The binding sites of Magnesium atom in the Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A (pdb code 4zfh). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 4 binding sites of Magnesium where determined in the Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A, PDB code: 4zfh:
Jump to Magnesium binding site number: 1; 2; 3; 4;

Magnesium binding site 1 out of 4 in the Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg201 b:18.4 occ:1.00 O A:VAL73 2.6 9.1 1.0 N A:LEU84 3.0 7.1 1.0 MG A:MG202 3.0 19.1 1.0 CB A:LEU84 3.2 20.0 1.0 CB A:ALA104 3.5 10.8 1.0 OD1 A:ASN74 3.6 11.7 1.0 CD A:PRO83 3.6 7.6 1.0 CB A:THR82 3.6 6.6 1.0 CA A:LEU84 3.7 7.6 1.0 OG1 A:THR82 3.7 6.2 1.0 C A:VAL73 3.8 10.1 1.0 N A:PRO83 3.8 7.3 1.0 C A:PRO83 4.0 7.4 1.0 CB A:PRO83 4.2 8.0 1.0 CA A:ALA104 4.2 11.1 1.0 CA A:ASN74 4.2 10.8 1.0 CG2 A:VAL73 4.2 20.0 1.0 CA A:PRO83 4.2 7.3 1.0 CG A:PRO83 4.3 8.0 1.0 C A:THR82 4.3 7.0 1.0 N A:ASN74 4.4 10.2 1.0 CG2 A:THR82 4.5 6.7 1.0 N A:ASP105 4.5 12.9 1.0 CA A:THR82 4.5 6.8 1.0 CG A:ASN74 4.6 11.7 1.0 CG A:LEU84 4.6 20.0 1.0 C A:ALA104 4.8 11.6 1.0 CD2 A:LEU84 4.9 20.0 1.0 C A:LEU84 4.9 6.9 1.0 CA A:VAL73 4.9 11.5 1.0 CD1 A:LEU99 4.9 10.7 1.0

Magnesium binding site 2 out of 4 in the Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:19.1 occ:1.00 O A:ASP105 2.6 11.1 1.0 OG1 A:THR82 2.8 6.2 1.0 MG A:MG201 3.0 18.4 1.0 CB A:THR82 3.4 6.6 1.0 N A:ASP105 3.5 12.9 1.0 CB A:ALA108 3.5 10.3 1.0 C A:ASP105 3.5 13.5 1.0 CG2 A:THR82 3.6 6.7 1.0 OD1 A:ASN74 3.6 11.7 1.0 CB A:LEU84 3.7 20.0 1.0 CA A:ASP105 3.8 13.2 1.0 CB A:ASP105 3.9 14.6 1.0 CD2 A:LEU84 4.0 20.0 1.0 C A:ALA104 4.4 11.6 1.0 CG A:LEU84 4.4 20.0 1.0 CB A:ALA104 4.5 10.8 1.0 N A:LEU84 4.6 7.1 1.0 CG A:ASN74 4.7 11.7 1.0 CA A:LEU84 4.7 7.6 1.0 CA A:ALA108 4.7 10.9 1.0 N A:VAL106 4.7 12.0 1.0 N A:ALA108 4.7 11.3 1.0 CA A:ALA104 4.7 11.1 1.0 CG A:PRO116 4.8 9.0 1.0 CG A:ASP105 4.8 16.8 1.0 CA A:THR82 4.9 6.8 1.0 ND1 A:HIS85 4.9 8.6 1.0

Magnesium binding site 3 out of 4 in the Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg203 b:19.2 occ:1.00 O A:VAL106 2.6 13.1 1.0 N A:LEU117 3.1 9.4 1.0 MG A:MG204 3.1 28.3 1.0 CB A:LEU117 3.3 20.0 1.0 OG1 A:THR115 3.4 9.3 1.0 CB A:THR115 3.5 8.6 1.0 CB A:ALA137 3.6 17.2 1.0 CD A:PRO116 3.6 8.7 1.0 OD1 A:ASN107 3.7 19.2 1.0 C A:VAL106 3.7 13.5 1.0 CA A:LEU117 3.7 10.4 1.0 N A:PRO116 3.7 8.6 1.0 C A:PRO116 4.0 8.8 1.0 CB A:PRO116 4.1 8.9 1.0 CA A:ALA137 4.2 17.0 1.0 CA A:PRO116 4.2 8.9 1.0 CG A:PRO116 4.2 9.0 1.0 C A:THR115 4.2 8.6 1.0 CG2 A:VAL106 4.3 13.3 1.0 CA A:ASN107 4.3 15.0 1.0 N A:ASN107 4.4 14.7 1.0 CA A:THR115 4.4 8.7 1.0 CG2 A:THR115 4.5 8.9 1.0 CG A:LEU117 4.6 20.0 1.0 CG A:ASN107 4.7 18.2 1.0 CA A:VAL106 4.7 13.8 1.0 N A:ASP138 4.8 21.9 1.0 CD2 A:LEU117 4.9 20.0 1.0 CD1 A:LEU132 4.9 9.5 1.0 CB A:LEU132 4.9 10.1 1.0 C A:ALA137 5.0 20.7 1.0 CD2 A:LEU132 5.0 9.4 1.0 CB A:VAL106 5.0 13.0 1.0 C A:LEU117 5.0 10.5 1.0

Magnesium binding site 4 out of 4 in the Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant -Y56A within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg204 b:28.3 occ:1.00 OG1 A:THR115 2.7 9.3 1.0 O A:ASP138 2.8 21.8 1.0 MG A:MG203 3.1 19.2 1.0 CB A:LEU117 3.4 20.0 1.0 N A:ASP138 3.4 21.9 1.0 CB A:THR115 3.5 8.6 1.0 OD1 A:ASN107 3.5 19.2 1.0 CD2 A:LEU117 3.5 20.0 1.0 C A:ASP138 3.6 23.4 1.0 CG2 A:THR115 3.7 8.9 1.0 CB A:ALA141 3.9 18.0 1.0 CA A:ASP138 3.9 24.2 1.0 CG A:LEU117 4.1 20.0 1.0 CB A:ALA137 4.2 17.2 1.0 CB A:ASP138 4.2 24.8 1.0 C A:ALA137 4.2 20.7 1.0 O A:HOH345 4.2 12.1 1.0 CA A:ALA137 4.5 17.0 1.0 CA A:LEU117 4.6 10.4 1.0 N A:LEU117 4.6 9.4 1.0 N A:VAL139 4.6 22.1 1.0 CG A:ASN107 4.7 18.2 1.0 ND1 A:HIS118 4.7 13.3 1.0 CE1 A:HIS118 4.9 13.0 1.0 CA A:THR115 4.9 8.7 1.0

P.Chuankhayan, S.Saoin, K.Chupradit, T.Wisitponchai, K.Intachai, K.Kitidee, S.Nangola, L.V.Sanghiran, S.S.Hong, P.Boulanger, C.Tayapiwatana, C.J.Chen. Crystal Structure of Artificial Ankyrin Repeat PROTEIN_ANK(Gag)1D4 Mutant- Y56A To Be Published.