Magnesium in PDB 4zi3: Bart-Like Domain of BARTL1/CCDC104 AA1-133 in Complex with ARL3FL Bound to Gppnhp in P1 21 1

The structure of Bart-Like Domain of BARTL1/CCDC104 AA1-133 in Complex with ARL3FL Bound to Gppnhp in P1 21 1, PDB code: 4zi3 was solved by M.Lokaj, C.Koerner, S.Koesling, A.Wittinghofer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.95 / 2.00
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	51.550, 67.720, 98.470, 90.00, 102.65, 90.00
R / Rfree (%)	18.9 / 24.3

The binding sites of Magnesium atom in the Bart-Like Domain of BARTL1/CCDC104 AA1-133 in Complex with ARL3FL Bound to Gppnhp in P1 21 1 (pdb code 4zi3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Bart-Like Domain of BARTL1/CCDC104 AA1-133 in Complex with ARL3FL Bound to Gppnhp in P1 21 1, PDB code: 4zi3:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Bart-Like Domain of BARTL1/CCDC104 AA1-133 in Complex with ARL3FL Bound to Gppnhp in P1 21 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Bart-Like Domain of BARTL1/CCDC104 AA1-133 in Complex with ARL3FL Bound to Gppnhp in P1 21 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:25.1 occ:1.00 O3G A:GNP301 2.0 18.5 1.0 OG1 A:THR48 2.1 22.7 1.0 O A:HOH429 2.1 26.8 1.0 O A:HOH433 2.1 25.5 1.0 O2B A:GNP301 2.1 21.2 1.0 OG1 A:THR31 2.1 23.2 1.0 CB A:THR48 3.1 26.1 1.0 PG A:GNP301 3.1 23.1 1.0 CB A:THR31 3.2 22.4 1.0 PB A:GNP301 3.3 22.9 1.0 N3B A:GNP301 3.4 21.5 1.0 N A:THR48 3.6 24.3 1.0 O2G A:GNP301 3.7 26.6 1.0 CA A:THR48 4.0 25.6 1.0 O1A A:GNP301 4.0 20.8 1.0 N A:THR31 4.1 21.8 1.0 OD2 A:ASP67 4.1 25.4 1.0 CA A:THR31 4.2 23.8 1.0 CG2 A:THR48 4.2 23.4 1.0 CG2 A:THR31 4.3 25.2 1.0 O A:THR46 4.3 25.1 1.0 OD1 A:ASP67 4.3 30.8 1.0 O1G A:GNP301 4.3 21.0 1.0 O1B A:GNP301 4.4 20.2 1.0 O A:HOH434 4.4 27.1 1.0 O3A A:GNP301 4.4 25.0 1.0 C A:PRO47 4.6 27.1 1.0 PA A:GNP301 4.6 27.1 1.0 CG A:ASP67 4.7 27.9 1.0 CA A:PRO47 4.8 26.2 1.0 O2A A:GNP301 4.8 22.7 1.0

Magnesium binding site 2 out of 2 in the Bart-Like Domain of BARTL1/CCDC104 AA1-133 in Complex with ARL3FL Bound to Gppnhp in P1 21 1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Bart-Like Domain of BARTL1/CCDC104 AA1-133 in Complex with ARL3FL Bound to Gppnhp in P1 21 1 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg302 b:44.2 occ:1.00 O B:HOH408 1.9 35.0 1.0 O1B B:GNP301 2.0 45.7 1.0 O1G B:GNP301 2.0 42.8 1.0 OG1 B:THR48 2.1 36.9 1.0 OG1 B:THR31 2.2 39.8 1.0 PG B:GNP301 2.9 49.5 1.0 PB B:GNP301 3.1 46.3 1.0 N3B B:GNP301 3.2 39.6 1.0 CB B:THR48 3.2 36.7 1.0 CB B:THR31 3.3 38.8 1.0 O2G B:GNP301 3.4 37.5 1.0 N B:THR48 3.6 40.2 1.0 O2A B:GNP301 4.0 34.3 1.0 N B:THR31 4.0 41.5 1.0 CA B:THR48 4.0 35.2 1.0 O2B B:GNP301 4.1 36.1 1.0 O3A B:GNP301 4.1 51.7 1.0 O3G B:GNP301 4.2 37.3 1.0 CA B:THR31 4.3 47.2 1.0 OD1 B:ASP67 4.3 40.6 1.0 OD2 B:ASP67 4.3 36.5 1.0 O B:THR46 4.3 46.7 1.0 CG2 B:THR48 4.3 39.7 1.0 CG2 B:THR31 4.4 43.7 1.0 PA B:GNP301 4.4 43.9 1.0 O D:HOH204 4.6 36.5 1.0 O1A B:GNP301 4.6 44.1 1.0 C B:PRO47 4.7 43.0 1.0 CG B:ASP67 4.7 47.5 1.0 CA B:PRO47 4.9 42.9 1.0

M.Lokaj, S.K.Kosling, C.Koerner, S.M.Lange, S.E.Van Beersum, J.Van Reeuwijk, R.Roepman, N.Horn, M.Ueffing, K.Boldt, A.Wittinghofer. The Interaction of CCDC104/BARTL1 with ARL3 and Implications For Ciliary Function. Structure V. 23 2122 2015.
ISSN: ISSN 0969-2126
PubMed: 26455799
DOI: 10.1016/J.STR.2015.08.016