Magnesium in PDB 5a77: Crystal Structure of the Homing Endonuclease I-Cvui in Complex with I-Crei Target (C1221) in the Presence of 2 Mm Mg Revealing Dna Cleaved

The structure of Crystal Structure of the Homing Endonuclease I-Cvui in Complex with I-Crei Target (C1221) in the Presence of 2 Mm Mg Revealing Dna Cleaved, PDB code: 5a77 was solved by R.Molina, P.Redondo, B.Lopezmendez, M.Villate, N.Merino, F.J.Blanco, J.Valton, S.Grizot, P.Duchateau, J.Prieto, G.Montoya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.539 / 2.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	48.573, 95.079, 52.145, 90.00, 103.71, 90.00
R / Rfree (%)	19.64 / 26.54

The binding sites of Magnesium atom in the Crystal Structure of the Homing Endonuclease I-Cvui in Complex with I-Crei Target (C1221) in the Presence of 2 Mm Mg Revealing Dna Cleaved (pdb code 5a77). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Homing Endonuclease I-Cvui in Complex with I-Crei Target (C1221) in the Presence of 2 Mm Mg Revealing Dna Cleaved, PDB code: 5a77:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Homing Endonuclease I-Cvui in Complex with I-Crei Target (C1221) in the Presence of 2 Mm Mg Revealing Dna Cleaved


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Homing Endonuclease I-Cvui in Complex with I-Crei Target (C1221) in the Presence of 2 Mm Mg Revealing Dna Cleaved within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1164 b:55.1 occ:1.00 OP1 C:DC514 2.1 50.5 1.0 O B:ALA22 2.3 45.4 1.0 OD2 A:ASP23 2.4 60.6 1.0 OP1 F:DG615 2.5 57.8 1.0 O A:HOH2009 2.8 49.4 1.0 C B:ALA22 3.4 42.9 1.0 CG A:ASP23 3.5 56.6 1.0 P C:DC514 3.5 50.7 1.0 OD1 B:ASP23 3.6 53.9 1.0 P F:DG615 3.7 60.8 1.0 OD1 A:ASP23 3.9 53.6 1.0 O3' C:DA513 3.9 46.4 1.0 CA B:ALA22 3.9 40.9 1.0 OP2 F:DG615 4.1 56.9 1.0 C5' C:DC514 4.1 42.6 1.0 O5' C:DC514 4.3 51.1 1.0 OP3 F:DG615 4.3 57.8 1.0 OE1 A:GLN50 4.4 46.0 1.0 C4' C:DC514 4.4 47.5 1.0 NE2 A:GLN50 4.4 41.6 1.0 N B:ASP23 4.5 43.5 1.0 CB B:ALA22 4.5 41.9 1.0 OP2 C:DC514 4.6 41.0 1.0 CG B:ASP23 4.6 51.1 1.0 CB A:ASP23 4.7 50.7 1.0 CA B:ASP23 4.8 47.6 1.0 CD A:GLN50 4.8 44.8 1.0 O3' D:DC614 4.9 48.8 1.0 O5' F:DG615 4.9 56.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Homing Endonuclease I-Cvui in Complex with I-Crei Target (C1221) in the Presence of 2 Mm Mg Revealing Dna Cleaved


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Homing Endonuclease I-Cvui in Complex with I-Crei Target (C1221) in the Presence of 2 Mm Mg Revealing Dna Cleaved within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1615 b:52.1 occ:1.00 OP1 D:DC614 2.0 46.2 1.0 OP2 E:DG515 2.1 56.2 1.0 O A:ALA22 2.3 41.8 1.0 OD2 B:ASP23 2.5 48.7 1.0 O A:HOH2002 3.1 47.8 1.0 P D:DC614 3.4 51.8 1.0 C A:ALA22 3.5 42.0 1.0 P E:DG515 3.5 59.6 1.0 CG B:ASP23 3.6 51.1 1.0 C5' D:DC614 3.7 49.0 1.0 OP3 E:DG515 3.8 50.7 1.0 O3' D:DA613 3.9 48.9 1.0 O5' D:DC614 4.0 51.0 1.0 OD1 B:ASP23 4.2 53.9 1.0 NE2 B:GLN50 4.2 46.3 1.0 OD1 A:ASP23 4.2 53.6 1.0 CA A:ALA22 4.3 41.3 1.0 C4' D:DC614 4.3 48.5 1.0 OP1 E:DG515 4.4 63.1 1.0 N A:ASP23 4.4 43.5 1.0 OE1 B:GLN50 4.5 47.5 1.0 O5' E:DG515 4.6 53.4 1.0 CA A:ASP23 4.6 49.0 1.0 OP2 D:DC614 4.6 42.3 1.0 CD B:GLN50 4.8 46.6 1.0 CB B:ASP23 4.8 48.1 1.0 C5' E:DG515 4.9 50.8 1.0 CB A:ALA22 5.0 42.5 1.0

R.Molina, P.Redondo, B.Lopez-Mendez, M.Villate, N.Merino, F.J.Blanco, J.Valton, S.Grizot, P.Duchateau, J.Prieto, G.Montoya. Crystal Structure of the Homing Endonuclease I-Cvui Provides A New Template For Genome Modification J.Biol.Chem. V. 290 28727 2015.
ISSN: ISSN 0021-9258
PubMed: 26363068
DOI: 10.1074/JBC.M115.678342