Magnesium in PDB 5acp: W228R-Investigation of the Impact From Residues W228 and Y233 in the Metallo-Beta-Lactamase Gim-1

The structure of W228R-Investigation of the Impact From Residues W228 and Y233 in the Metallo-Beta-Lactamase Gim-1, PDB code: 5acp was solved by S.Skagseth, T.J.Carlsen, G.E.K.Bjerga, J.Spencer, O.Samuelsen, H.-K.S.Leiros, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.83 / 1.98
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	38.757, 133.619, 40.702, 90.00, 95.26, 90.00
R / Rfree (%)	20.2 / 23.4

The structure of W228R-Investigation of the Impact From Residues W228 and Y233 in the Metallo-Beta-Lactamase Gim-1 also contains other interesting chemical elements:

Zinc

(Zn)

3 atoms

The binding sites of Magnesium atom in the W228R-Investigation of the Impact From Residues W228 and Y233 in the Metallo-Beta-Lactamase Gim-1 (pdb code 5acp). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the W228R-Investigation of the Impact From Residues W228 and Y233 in the Metallo-Beta-Lactamase Gim-1, PDB code: 5acp:

Magnesium binding site 1 out of 1 in the W228R-Investigation of the Impact From Residues W228 and Y233 in the Metallo-Beta-Lactamase Gim-1


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of W228R-Investigation of the Impact From Residues W228 and Y233 in the Metallo-Beta-Lactamase Gim-1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1298 b:42.0 occ:1.00 OG A:SER139 2.5 30.5 1.0 O A:ASP199 2.7 35.9 1.0 HB2 A:ASP199 2.8 42.7 1.0 O A:HOH2099 2.8 34.5 1.0 HG A:SER139 2.9 36.6 1.0 HB3 A:LEU141 2.9 46.0 1.0 HA A:ASP199 2.9 42.0 1.0 HD21 A:LEU141 3.3 50.3 1.0 CB A:SER139 3.3 32.4 1.0 HG21 A:ILE201 3.3 39.1 1.0 HB2 A:SER139 3.3 38.9 1.0 HB3 A:SER139 3.3 38.9 1.0 C A:ASP199 3.4 33.4 1.0 CA A:ASP199 3.4 35.0 1.0 HD1 A:TYR191 3.5 47.2 1.0 CB A:ASP199 3.5 35.6 1.0 H A:THR142 3.7 40.9 1.0 CD1 A:TYR191 3.8 39.3 1.0 OG1 A:THR142 3.9 31.5 1.0 CG2 A:ILE201 3.9 32.6 1.0 HG22 A:ILE201 3.9 39.1 1.0 CB A:LEU141 3.9 38.4 1.0 HB3 A:ASP199 3.9 42.7 1.0 HG23 A:ILE201 3.9 39.1 1.0 HE1 A:TYR191 4.0 48.3 1.0 HG1 A:THR142 4.0 37.8 1.0 O A:HOH2053 4.1 38.7 1.0 CE1 A:TYR191 4.1 40.2 1.0 CD2 A:LEU141 4.1 41.9 1.0 HB2 A:LEU141 4.3 46.0 1.0 HG A:LEU141 4.3 47.8 1.0 HB2 A:TYR191 4.3 48.4 1.0 N A:THR142 4.3 34.1 1.0 H A:LEU141 4.3 42.6 1.0 CG A:LEU141 4.4 39.8 1.0 HD23 A:LEU141 4.5 50.3 1.0 CG A:TYR191 4.6 40.2 1.0 N A:ASN200 4.7 32.8 1.0 CA A:SER139 4.7 33.5 1.0 O A:HOH2037 4.7 29.9 1.0 CG A:ASP199 4.7 38.2 1.0 CA A:LEU141 4.8 36.3 1.0 HD22 A:LEU141 4.8 50.3 1.0 N A:ASP199 4.8 33.5 1.0 N A:LEU141 4.9 35.5 1.0 H A:SER139 4.9 41.4 1.0 CB A:THR142 4.9 32.4 1.0 CB A:TYR191 5.0 40.3 1.0 C A:LEU141 5.0 35.2 1.0

S.Skagseth, T.J.Carlsen, G.E.K.Bjerga, J.Spencer, O.Samuelsen, H.S.Leiros. Role of Residues W228 and Y233 in the Structure and Activity of Metallo-Beta-Lactamase Gim-1. Antimicrob.Agents Chemother. V. 60 990 2015.
ISSN: ISSN 0066-4804
PubMed: 26643332
DOI: 10.1128/AAC.02017-15