Magnesium in PDB 5c2v: Kuenenia Stuttgartiensis Hydrazine Synthase

The structure of Kuenenia Stuttgartiensis Hydrazine Synthase, PDB code: 5c2v was solved by A.Dietl, C.Ferousi, W.J.Maalcke, A.Menzel, S.De Vries, J.T.Keltjens, M.S.M.Jetten, B.Kartal, T.R.M.Barends, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.70
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	464.540, 464.540, 145.750, 90.00, 90.00, 120.00
R / Rfree (%)	23.5 / 27.1

The structure of Kuenenia Stuttgartiensis Hydrazine Synthase also contains other interesting chemical elements:

Zinc	(Zn)	2 atoms
Iron	(Fe)	8 atoms
Calcium	(Ca)	12 atoms
Chlorine	(Cl)	3 atoms

The binding sites of Magnesium atom in the Kuenenia Stuttgartiensis Hydrazine Synthase (pdb code 5c2v). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Kuenenia Stuttgartiensis Hydrazine Synthase, PDB code: 5c2v:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Kuenenia Stuttgartiensis Hydrazine Synthase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Kuenenia Stuttgartiensis Hydrazine Synthase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:33.0 occ:1.00 OE1 B:GLU253 1.9 24.8 0.5 O A:HOH1146 2.0 49.5 1.0 O A:HOH1137 2.1 53.3 1.0 O B:HOH524 2.4 34.6 1.0 O B:HOH504 2.5 30.2 1.0 O B:HOH513 2.7 38.1 1.0 CD B:GLU253 3.1 27.9 0.5 ND2 A:ASN290 3.5 42.0 1.0 OE2 B:GLU253 3.7 35.5 0.5 CG B:GLU253 3.7 30.8 0.5 O C:HOH551 3.7 37.9 1.0 CB B:GLU253 4.2 41.6 0.5 CG B:GLU253 4.3 31.5 0.5 CB B:GLU253 4.3 42.1 0.5 CB A:TYR295 4.4 27.6 1.0 CG A:ASN290 4.5 47.7 1.0 CA B:GLU253 4.5 44.6 0.5 CA B:GLU253 4.5 44.8 0.5 OE1 B:GLU255 4.5 36.6 1.0 OD1 A:ASN290 4.6 43.2 1.0 O B:CYS252 4.6 49.0 1.0 O B:HOH556 4.7 43.0 1.0 CG A:TYR295 4.7 27.5 1.0 OE2 B:GLU255 4.7 36.3 1.0 O B:HOH522 4.8 44.9 1.0 CB C:ARG174 4.8 37.8 1.0 CG C:ARG174 5.0 40.4 1.0

Magnesium binding site 2 out of 2 in the Kuenenia Stuttgartiensis Hydrazine Synthase


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Kuenenia Stuttgartiensis Hydrazine Synthase within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg401 b:47.1 occ:1.00 OE2 E:GLU253 2.0 47.0 0.5 O D:HOH1093 2.1 91.6 1.0 O E:HOH508 2.2 52.4 1.0 O E:HOH512 2.3 52.2 1.0 O E:HOH530 2.3 51.8 1.0 O E:HOH502 2.6 49.0 1.0 CD E:GLU253 3.1 40.4 0.5 OD1 D:ASN290 3.5 82.2 1.0 OE1 E:GLU253 3.6 36.6 0.5 CG E:GLU253 3.8 40.1 0.5 CG E:GLU253 4.3 40.3 0.5 CB E:GLU253 4.3 47.6 0.5 CB E:GLU253 4.4 46.9 0.5 CB D:TYR295 4.4 56.4 1.0 CG D:ASN290 4.4 69.9 1.0 O E:CYS252 4.5 50.9 1.0 CA E:GLU253 4.5 49.5 0.5 OE1 E:GLU255 4.5 61.4 1.0 OE2 E:GLU255 4.6 57.1 1.0 CA E:GLU253 4.6 49.3 0.5 CG D:TYR295 4.8 34.8 1.0 ND2 D:ASN290 4.8 55.1 1.0 CB F:ARG174 4.8 40.7 1.0 CG F:ARG174 4.9 36.8 1.0 CD E:GLU255 5.0 46.8 1.0

A.Dietl, C.Ferousi, W.J.Maalcke, A.Menzel, S.De Vries, J.T.Keltjens, M.S.Jetten, B.Kartal, T.R.Barends. The Inner Workings of the Hydrazine Synthase Multiprotein Complex. Nature V. 527 394 2015.
ISSN: ESSN 1476-4687
PubMed: 26479033
DOI: 10.1038/NATURE15517