Magnesium in PDB 5cnn: Crystal Structure of the Egfr Kinase Domain Mutant I682Q

All present enzymatic activity of Crystal Structure of the Egfr Kinase Domain Mutant I682Q:
2.7.10.1;

The structure of Crystal Structure of the Egfr Kinase Domain Mutant I682Q, PDB code: 5cnn was solved by E.Kovacs, R.Das, A.Mirza, N.Jura, T.Barros, J.Kuriyan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.51 / 1.90
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	155.887, 72.045, 76.774, 90.00, 113.32, 90.00
R / Rfree (%)	18.2 / 21.1

The binding sites of Magnesium atom in the Crystal Structure of the Egfr Kinase Domain Mutant I682Q (pdb code 5cnn). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of the Egfr Kinase Domain Mutant I682Q, PDB code: 5cnn:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of the Egfr Kinase Domain Mutant I682Q


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of the Egfr Kinase Domain Mutant I682Q within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1102 b:26.3 occ:1.00 O2B A:ANP1101 1.8 25.1 1.0 O1A A:ANP1101 1.9 23.7 1.0 O A:HOH1201 2.0 36.2 1.0 OD1 A:ASN818 2.1 24.8 1.0 OD2 A:ASP831 2.1 37.5 1.0 O A:HOH1272 2.1 28.2 1.0 PA A:ANP1101 3.1 24.8 1.0 PB A:ANP1101 3.1 28.1 1.0 O3G A:ANP1101 3.2 34.5 1.0 CG A:ASN818 3.2 24.1 1.0 CG A:ASP831 3.3 28.7 1.0 O3A A:ANP1101 3.5 27.9 1.0 O5' A:ANP1101 3.8 21.7 1.0 ND2 A:ASN818 3.8 19.8 1.0 NZ A:LYS721 3.9 37.8 1.0 CB A:ASP831 4.0 23.4 1.0 PG A:ANP1101 4.0 24.6 1.0 N3B A:ANP1101 4.1 19.2 1.0 O1B A:ANP1101 4.2 29.4 1.0 OD1 A:ASP831 4.2 43.2 1.0 O A:ARG817 4.3 18.6 1.0 O2G A:ANP1101 4.4 24.6 1.0 O2A A:ANP1101 4.4 23.1 1.0 CG2 A:THR830 4.4 21.8 1.0 CB A:ASN818 4.4 16.2 1.0 CA A:ASN818 4.6 19.0 1.0 O A:HOH1256 4.8 24.7 1.0 CE A:LYS721 4.8 41.6 1.0 C3' A:ANP1101 4.8 29.3 1.0 C A:ARG817 4.9 17.7 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of the Egfr Kinase Domain Mutant I682Q


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of the Egfr Kinase Domain Mutant I682Q within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg1102 b:21.0 occ:1.00 O3G B:ANP1101 1.9 29.6 1.0 O1A B:ANP1101 2.0 25.6 1.0 O2B B:ANP1101 2.0 26.6 1.0 O B:HOH1259 2.1 25.9 1.0 OD2 B:ASP831 2.1 27.6 1.0 OD1 B:ASN818 2.1 23.4 1.0 PB B:ANP1101 3.1 26.3 1.0 PA B:ANP1101 3.2 26.9 1.0 CG B:ASN818 3.2 23.4 1.0 CG B:ASP831 3.3 31.1 1.0 PG B:ANP1101 3.3 25.5 1.0 O3A B:ANP1101 3.4 24.8 1.0 N3B B:ANP1101 3.7 25.3 1.0 ND2 B:ASN818 3.7 20.7 1.0 O5' B:ANP1101 3.9 24.6 1.0 NZ B:LYS721 4.0 30.1 1.0 OD1 B:ASP831 4.1 32.2 1.0 O2G B:ANP1101 4.2 26.8 1.0 O1G B:ANP1101 4.3 24.3 1.0 O B:HOH1273 4.3 32.6 1.0 CB B:ASP831 4.3 22.8 1.0 O1B B:ANP1101 4.4 28.7 1.0 O2A B:ANP1101 4.4 24.1 1.0 CB B:ASN818 4.5 15.2 1.0 O B:ARG817 4.5 16.5 1.0 O B:HOH1262 4.5 29.4 1.0 CA B:ASN818 4.7 17.0 1.0 CG2 B:THR830 4.8 19.2 1.0 O B:HOH1268 4.9 33.4 1.0 C B:ARG817 5.0 22.1 1.0

E.Kovacs, R.Das, Q.Wang, T.S.Collier, A.Cantor, Y.Huang, K.Wong, A.Mirza, T.Barros, P.Grob, N.Jura, R.Bose, J.Kuriyan. Analysis of the Role of the C-Terminal Tail in the Regulation of the Epidermal Growth Factor Receptor. Mol.Cell.Biol. V. 35 3083 2015.
ISSN: ESSN 1098-5549
PubMed: 26124280
DOI: 10.1128/MCB.00248-15