Magnesium in PDB 5di3: Crystal Structure of ARL13B in Complex with ARL3 of Chlamydomonas Reinhardtii

The structure of Crystal Structure of ARL13B in Complex with ARL3 of Chlamydomonas Reinhardtii, PDB code: 5di3 was solved by K.Gotthardt, M.Lokaj, N.Falk, C.Koerner, A.Giessl, A.Wittinghofer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.84 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	57.100, 68.800, 120.000, 90.00, 90.00, 90.00
R / Rfree (%)	19.9 / 23.6

The binding sites of Magnesium atom in the Crystal Structure of ARL13B in Complex with ARL3 of Chlamydomonas Reinhardtii (pdb code 5di3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of ARL13B in Complex with ARL3 of Chlamydomonas Reinhardtii, PDB code: 5di3:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of ARL13B in Complex with ARL3 of Chlamydomonas Reinhardtii


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of ARL13B in Complex with ARL3 of Chlamydomonas Reinhardtii within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg302 b:47.9 occ:1.00 O B:HOH404 2.0 60.8 1.0 OG1 B:THR33 2.0 44.9 1.0 O1G B:GNP301 2.1 50.7 1.0 O1B B:GNP301 2.2 49.9 1.0 O B:HOH405 2.2 40.1 1.0 OG1 B:THR50 2.3 51.0 1.0 CB B:THR33 3.2 46.9 1.0 CB B:THR50 3.2 53.3 1.0 PG B:GNP301 3.3 49.7 1.0 PB B:GNP301 3.4 56.1 1.0 N3B B:GNP301 3.6 47.6 1.0 N B:THR33 3.8 45.2 1.0 OD1 B:ASP69 3.9 46.4 1.0 N B:THR50 4.0 47.3 1.0 O2G B:GNP301 4.0 48.2 1.0 CA B:THR33 4.0 45.9 1.0 OD2 B:ASP69 4.1 53.4 1.0 CA B:THR50 4.2 52.9 1.0 CG2 B:THR33 4.3 40.0 1.0 O1A B:GNP301 4.3 56.1 1.0 O2B B:GNP301 4.3 48.6 1.0 CG2 B:THR50 4.3 42.8 1.0 O3G B:GNP301 4.4 52.6 1.0 CG B:ASP69 4.5 51.4 1.0 O3A B:GNP301 4.5 48.2 1.0 O B:LEU70 4.5 48.2 1.0 O B:THR48 4.6 45.0 1.0 PA B:GNP301 4.7 54.1 1.0 CB B:LYS32 4.7 39.4 1.0 O2A B:GNP301 4.7 45.1 1.0 CE B:LYS32 4.7 40.1 1.0 O B:HOH419 4.8 47.9 1.0 C B:PRO49 4.8 51.3 1.0 C B:LYS32 4.9 44.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of ARL13B in Complex with ARL3 of Chlamydomonas Reinhardtii


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of ARL13B in Complex with ARL3 of Chlamydomonas Reinhardtii within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg202 b:45.8 occ:1.00 O1G A:GNP201 2.0 49.9 1.0 OG1 A:THR30 2.1 41.4 1.0 O A:HOH301 2.3 47.6 1.0 O A:HOH303 2.3 42.4 1.0 O1B A:GNP201 2.4 53.5 1.0 OG1 A:THR47 2.4 39.1 1.0 CB A:THR47 2.9 45.8 1.0 PG A:GNP201 3.3 56.5 1.0 CB A:THR30 3.4 50.9 1.0 PB A:GNP201 3.5 49.8 1.0 N3B A:GNP201 3.7 55.1 1.0 N A:THR47 3.9 50.4 1.0 CG2 A:THR47 3.9 42.9 1.0 O2G A:GNP201 3.9 52.4 1.0 CA A:THR47 4.0 54.6 1.0 O A:ILE67 4.0 49.9 1.0 OD1 A:ASP66 4.0 61.9 1.0 N A:THR30 4.0 47.1 1.0 OD2 A:ASP66 4.2 62.7 1.0 CA A:THR30 4.3 43.2 1.0 O2B A:GNP201 4.3 52.5 1.0 O3G A:GNP201 4.4 55.2 1.0 CG2 A:THR30 4.4 48.6 1.0 O A:HOH302 4.5 44.7 1.0 O1A A:GNP201 4.5 57.0 1.0 CG A:ASP66 4.5 63.2 1.0 O A:THR45 4.6 47.2 1.0 C A:ILE67 4.6 49.7 1.0 O3A A:GNP201 4.7 56.9 1.0 CA A:GLY68 4.7 55.0 1.0 CE A:LYS29 4.7 50.4 1.0 C A:PRO46 4.8 46.5 1.0 CB A:LYS29 4.8 49.7 1.0 PA A:GNP201 4.9 49.6 1.0 N A:GLY68 5.0 55.3 1.0

K.Gotthardt, M.Lokaj, C.Koerner, N.Falk, A.Giel, A.Wittinghofer. A G-Protein Activation Cascade From ARL13B to ARL3 and Implications For Ciliary Targeting of Lipidated Proteins. Elife V. 4 2015.
ISSN: ESSN 2050-084X
PubMed: 26551564
DOI: 10.7554/ELIFE.11859