Magnesium in PDB 5do9: Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q

The structure of Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q, PDB code: 5do9 was solved by V.G.Taylor, J.J.G.Tesmer, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.60
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	173.946, 95.881, 112.898, 90.00, 94.31, 90.00
R / Rfree (%)	17.8 / 22.6

The structure of Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q also contains other interesting chemical elements:

Fluorine	(F)	12 atoms
Aluminium	(Al)	3 atoms

The binding sites of Magnesium atom in the Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q (pdb code 5do9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q, PDB code: 5do9:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg403 b:25.2 occ:1.00 F3 A:ALF402 2.0 27.7 1.0 OG1 A:THR186 2.0 25.2 1.0 O A:HOH512 2.0 23.6 1.0 O A:HOH509 2.0 23.1 1.0 O2B A:GDP401 2.0 23.5 1.0 OG A:SER53 2.1 23.0 1.0 CB A:THR186 2.9 25.8 1.0 CB A:SER53 3.0 24.5 1.0 PB A:GDP401 3.1 24.1 1.0 O1B A:GDP401 3.3 23.8 1.0 F1 A:ALF402 3.3 28.1 1.0 AL A:ALF402 3.4 28.1 1.0 N A:SER53 3.8 24.8 1.0 N A:THR186 3.8 26.8 1.0 CA A:THR186 4.0 26.6 1.0 CA A:SER53 4.0 25.4 1.0 CG2 A:THR186 4.0 25.8 1.0 O1A A:GDP401 4.1 26.6 1.0 O3A A:GDP401 4.1 24.3 1.0 OD2 A:ASP205 4.3 31.4 1.0 O3B A:GDP401 4.3 22.6 1.0 O A:VAL184 4.3 31.0 1.0 OD1 A:ASP205 4.4 31.4 1.0 F2 A:ALF402 4.4 26.5 1.0 PA A:GDP401 4.4 26.0 1.0 O A:HOH525 4.5 30.1 1.0 NH1 A:ARG183 4.6 33.0 1.0 O A:VAL206 4.6 26.4 1.0 O2A A:GDP401 4.7 25.9 1.0 CG A:ASP205 4.7 31.2 1.0 C A:PRO185 4.8 26.6 1.0 C A:LYS52 4.8 26.4 1.0 CB A:LYS52 4.9 26.2 1.0

Magnesium binding site 2 out of 3 in the Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg403 b:25.8 occ:1.00 O C:HOH502 1.8 27.4 1.0 F3 C:ALF402 1.9 24.3 1.0 O2B C:GDP401 2.0 22.0 1.0 OG1 C:THR186 2.0 30.2 1.0 O C:HOH505 2.1 24.0 1.0 OG C:SER53 2.3 25.7 1.0 PB C:GDP401 2.9 23.8 1.0 O3B C:GDP401 3.0 22.4 1.0 F1 C:ALF402 3.1 27.7 1.0 CB C:THR186 3.2 29.2 1.0 AL C:ALF402 3.2 25.7 1.0 CB C:SER53 3.3 27.9 1.0 N C:SER53 3.7 26.9 1.0 O2A C:GDP401 3.7 25.8 1.0 N C:THR186 3.9 30.8 1.0 O3A C:GDP401 4.0 24.9 1.0 CA C:SER53 4.1 27.9 1.0 CA C:THR186 4.1 29.2 1.0 O1B C:GDP401 4.1 22.7 1.0 F2 C:ALF402 4.2 24.3 1.0 CG2 C:THR186 4.2 28.9 1.0 PA C:GDP401 4.3 25.9 1.0 O D:HOH203 4.3 35.7 1.0 O C:HOH517 4.3 26.3 1.0 O C:VAL206 4.4 25.9 1.0 OD1 C:ASP205 4.4 39.6 1.0 O C:VAL184 4.5 35.5 1.0 NH1 C:ARG183 4.5 32.1 1.0 O1A C:GDP401 4.6 25.0 1.0 OD2 C:ASP205 4.6 38.1 1.0 C C:LYS52 4.8 25.4 1.0 CB C:LYS52 4.8 24.0 1.0 NZ C:LYS52 4.8 23.5 1.0 F4 C:ALF402 4.9 24.9 1.0 CE C:LYS52 4.9 23.2 1.0 C C:PRO185 4.9 32.0 1.0 CG C:ASP205 4.9 34.7 1.0

Magnesium binding site 3 out of 3 in the Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Structure of Regulator of G Protein Signaling 8 (RGS8) in Complex with ALF4-Activated Galpha-Q within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg403 b:22.9 occ:1.00 F3 E:ALF402 1.9 26.9 1.0 O E:HOH512 1.9 21.8 1.0 OG1 E:THR186 2.0 24.4 1.0 O2B E:GDP401 2.1 20.2 1.0 O E:HOH504 2.1 28.6 1.0 OG E:SER53 2.2 20.3 1.0 PB E:GDP401 3.0 20.3 1.0 CB E:THR186 3.0 25.5 1.0 O1B E:GDP401 3.1 18.9 1.0 F1 E:ALF402 3.1 27.3 1.0 CB E:SER53 3.1 22.2 1.0 AL E:ALF402 3.3 28.5 1.0 N E:SER53 3.7 24.0 1.0 N E:THR186 3.9 25.5 1.0 CA E:SER53 4.0 23.7 1.0 CG2 E:THR186 4.1 26.0 1.0 O3A E:GDP401 4.1 21.9 1.0 CA E:THR186 4.1 26.1 1.0 O1A E:GDP401 4.2 22.1 1.0 O E:HOH555 4.2 22.0 1.0 O3B E:GDP401 4.2 18.6 1.0 F2 E:ALF402 4.3 27.0 1.0 OD2 E:ASP205 4.3 31.1 1.0 OD1 E:ASP205 4.3 32.4 1.0 O E:VAL206 4.4 25.1 1.0 PA E:GDP401 4.5 23.8 1.0 O E:VAL184 4.5 28.8 1.0 O2A E:GDP401 4.7 23.7 1.0 CB E:LYS52 4.7 23.9 1.0 CG E:ASP205 4.7 30.5 1.0 C E:LYS52 4.8 24.6 1.0 NH1 E:ARG183 4.9 29.1 1.0 F4 E:ALF402 4.9 25.5 1.0 C E:PRO185 4.9 25.8 1.0

V.G.Taylor, P.A.Bommarito, J.J.Tesmer. Structure of the Regulator of G Protein Signaling 8 (RGS8)-G Alpha Q Complex: Molecular Basis For G Alpha Selectivity. J.Biol.Chem. V. 291 5138 2016.
ISSN: ESSN 1083-351X
PubMed: 26755720
DOI: 10.1074/JBC.M115.712075