Magnesium in PDB 5eb8: Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27

All present enzymatic activity of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27:
3.2.1.8;

The structure of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27, PDB code: 5eb8 was solved by P.Mahanta, A.Bhardwaj, V.S.Reddy, S.Ramakumar, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	88.38 / 2.22
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	55.270, 77.320, 176.750, 90.00, 90.00, 90.00
R / Rfree (%)	16.8 / 21.4

The structure of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Magnesium atom in the Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 (pdb code 5eb8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27, PDB code: 5eb8:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:10.0 occ:1.00 O A:ARG351 2.1 21.4 1.0 OD1 A:ASN292 2.3 23.0 1.0 O A:HOH652 2.3 23.7 1.0 O A:HOH564 2.3 15.6 1.0 OD1 A:ASP354 2.4 26.6 1.0 O A:HOH504 2.4 22.2 1.0 O A:HOH558 2.5 16.3 1.0 C A:ARG351 3.3 20.5 1.0 CG A:ASP354 3.4 23.8 1.0 CG A:ASN292 3.5 21.9 1.0 OD2 A:ASP354 3.7 24.6 1.0 CA A:ARG351 4.2 21.4 1.0 O A:HOH584 4.2 14.8 1.0 N A:ILE352 4.2 19.3 1.0 CB A:ASN292 4.3 21.3 1.0 CA A:ILE352 4.3 18.4 1.0 CA A:ASN292 4.4 20.4 1.0 O A:HOH729 4.4 33.1 1.0 ND2 A:ASN292 4.5 22.7 1.0 O A:ASP354 4.5 21.8 1.0 O A:ASN292 4.5 20.1 1.0 OE2 A:GLU296 4.6 28.2 1.0 CG A:GLU296 4.7 25.6 1.0 CB A:ARG351 4.7 23.1 1.0 C A:ASP354 4.7 22.8 1.0 CB A:ASP354 4.8 23.1 1.0 C A:ASN292 4.9 20.4 1.0 C A:ILE352 4.9 18.2 1.0 CD A:GLU296 5.0 27.4 1.0 N A:ASP354 5.0 21.0 1.0

Magnesium binding site 2 out of 2 in the Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:16.1 occ:1.00 O B:ARG351 2.2 25.7 1.0 OD1 B:ASN292 2.3 25.1 1.0 O B:HOH560 2.4 21.7 1.0 O B:HOH593 2.4 26.0 1.0 OD1 B:ASP354 2.4 25.1 1.0 O B:HOH599 2.5 22.6 1.0 O B:HOH553 2.5 28.6 1.0 C B:ARG351 3.4 24.4 1.0 CG B:ASN292 3.5 25.0 1.0 CG B:ASP354 3.5 25.0 1.0 OD2 B:ASP354 3.8 24.9 1.0 CB B:ASN292 4.2 23.7 1.0 CA B:ARG351 4.2 25.3 1.0 CA B:ASN292 4.3 23.4 1.0 N B:ILE352 4.3 22.8 1.0 OE2 B:GLU296 4.3 31.2 1.0 CA B:ILE352 4.3 22.3 1.0 O B:HOH561 4.5 22.3 1.0 CG B:GLU296 4.5 28.9 1.0 O B:ASN292 4.5 24.6 1.0 ND2 B:ASN292 4.5 26.1 1.0 O B:ASP354 4.6 23.1 1.0 O B:HOH642 4.7 37.9 1.0 C B:ASP354 4.7 24.0 1.0 CB B:ARG351 4.7 26.4 1.0 C B:ASN292 4.8 24.3 1.0 CD B:GLU296 4.8 31.2 1.0 CB B:ASP354 4.8 24.3 1.0 C B:ILE352 5.0 22.0 1.0

P.Mahanta, A.Bhardwaj, V.S.Reddy, S.Ramakumar. Crystal Structure of Aromatic Mutant (F4W) of An Alkali Thermostable GH10 Xylanase From Bacillus Sp. Ng-27 To Be Published.